scholarly journals Erratum: Corrigendum: Conformational rearrangements in the transmembrane domain of CNGA1 channels revealed by single-molecule force spectroscopy

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Sourav Maity ◽  
Monica Mazzolini ◽  
Manuel Arcangeletti ◽  
Alejandro Valbuena ◽  
Paolo Fabris ◽  
...  
Keyword(s):  
Single Molecule ◽  
Transmembrane Domain ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy ◽  
Conformational Rearrangements ◽  
Cnga1 Channels

scholarly journals Conformational rearrangements in the transmembrane domain of CNGA1 channels revealed by single-molecule force spectroscopy

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Sourav Maity ◽  
Monica Mazzolini ◽  
Manuel Arcangeletti ◽  
Alejandro Valbuena ◽  
Paolo Fabris ◽  
...  
Keyword(s):  
Single Molecule ◽  
Conformational Changes ◽  
Transmembrane Domain ◽  
Force Spectroscopy ◽  
Dynamic Structure ◽  
Transmembrane Domains ◽  
Structural Studies ◽  
Single Molecule Force Spectroscopy ◽  
Conformational Rearrangements ◽  
Α Helix

Abstract Cyclic nucleotide-gated (CNG) channels are activated by binding of cyclic nucleotides. Although structural studies have identified the channel pore and selectivity filter, conformation changes associated with gating remain poorly understood. Here we combine single-molecule force spectroscopy (SMFS) with mutagenesis, bioinformatics and electrophysiology to study conformational changes associated with gating. By expressing functional channels with SMFS fingerprints in Xenopus laevis oocytes, we were able to investigate gating of CNGA1 in a physiological-like membrane. Force spectra determined that the S4 transmembrane domain is mechanically coupled to S5 in the open state, but S3 in the closed state. We also show there are multiple pathways for the unfolding of the transmembrane domains, probably caused by a different degree of α-helix folding. This approach demonstrates that CNG transmembrane domains have dynamic structure and establishes SMFS as a tool for probing conformational change in ion channels.

scholarly journals Single Molecule Force Spectroscopy of CNGA1 Channels In Situ

2013 ◽  
Vol 104 (2) ◽  
pp. 167a
Author(s):  
Sourav Maity ◽  
Alejandro Valbuena ◽  
Monica Mazzolini ◽  
Vincent Torre
Keyword(s):  
Single Molecule ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy ◽  
Cnga1 Channels

scholarly journals Single Molecule Force Spectroscopy of CNGA1 Channels “In Situ” Reveals Major Conformational Changes upon Gating

2014 ◽  
Vol 106 (2) ◽  
pp. 392a
Author(s):  
Sourav Maity ◽  
Monica Mazzolini ◽  
Paolo Fabris ◽  
Marco Lazzarino ◽  
Alejandro Valbuena ◽  
...  
Keyword(s):  
Single Molecule ◽  
Conformational Changes ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy ◽  
Cnga1 Channels

scholarly journals Single Molecule Force Spectroscopy of Siloxanes

2021 ◽  
Vol 714 (3) ◽  
pp. 032023
Author(s):  
Ling Chen ◽  
Liya Yang ◽  
Chunxia Wang ◽  
Ting Zhu
Keyword(s):  
Single Molecule ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy

Pulling Genetic RNA out of Tobacco Mosaic Virus Using Single-Molecule Force Spectroscopy

2010 ◽  
Vol 132 (32) ◽  
pp. 11036-11038 ◽  
Author(s):  
Ningning Liu ◽  
Bo Peng ◽  
Yuan Lin ◽  
Zhaohui Su ◽  
Zhongwei Niu ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Single Molecule ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy

The Nature of the Force-Induced Conformation Transition of dsDNA Studied by Using Single Molecule Force Spectroscopy

Langmuir ◽  
2010 ◽  
Vol 26 (12) ◽  
pp. 9491-9496 ◽  
Author(s):  
Ningning Liu ◽  
Tianjia Bu ◽  
Yu Song ◽  
Wei Zhang ◽  
Jinjing Li ◽  
...  
Keyword(s):  
Single Molecule ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy ◽  
Conformation Transition
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