scholarly journals The E3 ubiquitin ligase and RNA-binding protein ZNF598 orchestrates ribosome quality control of premature polyadenylated mRNAs

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Aitor Garzia ◽  
Seyed Mehdi Jafarnejad ◽  
Cindy Meyer ◽  
Clément Chapat ◽  
Tasos Gogakos ◽  
...  
Keyword(s):  
Quality Control ◽  
Ubiquitin Ligase ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
E3 Ubiquitin Ligase

scholarly journals The RNA-binding protein HuR is a novel target of Pirh2 E3 ubiquitin ligase

2021 ◽  
Vol 12 (6) ◽  
Author(s):  
Alexandra Daks ◽  
Alexey Petukhov ◽  
Olga Fedorova ◽  
Oleg Shuvalov ◽  
Alena Kizenko ◽  
...  
Keyword(s):  
Heat Shock ◽  
Ubiquitin Ligase ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
E3 Ubiquitin Ligase ◽  
Ring Finger ◽  
P53 Family ◽  
Associated Proteins ◽  
Novel Target

AbstractThe RING-finger protein Pirh2 is a p53 family-specific E3 ubiquitin ligase. Pirh2 also ubiquitinates several other important cellular factors and is involved in carcinogenesis. However, its functional role in other cellular processes is poorly understood. To address this question, we performed a proteomic search for novel interacting partners of Pirh2. Using the GST-pulldown approach combined with LC-MS/MS, we revealed 225 proteins that interacted with Pirh2. We found that, according to the GO description, a large group of Pirh2-associated proteins belonged to the RNA metabolism group. Importantly, one of the identified proteins from that group was an RNA-binding protein ELAVL1 (HuR), which is involved in the regulation of splicing and protein stability of several oncogenic proteins. We demonstrated that Pirh2 ubiquitinated the HuR protein facilitating its proteasome-mediated degradation in cells. Importantly, the Pirh2-mediated degradation of HuR occurred in response to heat shock, thereby affecting the survival rate of HeLa cells under elevated temperature. Functionally, Pirh2-mediated degradation of HuR augmented the level of c-Myc expression, whose RNA level is otherwise attenuated by HuR. Taken together, our data indicate that HuR is a new target of Pirh2 and this functional interaction contributes to the heat-shock response of cancer cells affecting their survival.

scholarly journals Molecular Mechanisms of the Interaction between the RNA-Binding Protein Nab2 and the Nuclear Basket Protein Mlp1 in mRNA Quality Control

2019 ◽  
Vol 116 (3) ◽  
pp. 203a
Author(s):  
Mohammad Soheilypour ◽  
Mohaddeseh Peyro ◽  
Hengameh Shams ◽  
Stephanie Rider ◽  
Ali R. Kaazempur-Mofrad ◽  
...  
Keyword(s):  
Quality Control ◽  
Molecular Mechanisms ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein

235: The RNA-Binding Protein IMP3: A Novel Molecular Marker Predicts Progression of Superficial (TA and T1) Urothelial Carcinomas of Bladder

2007 ◽  
Vol 177 (4S) ◽  
pp. 78-79
Author(s):  
Lioudmila Sitnikova ◽  
Gary Mendese ◽  
Qin Lui ◽  
Bruce A. Woda ◽  
Di Lu ◽  
...  
Keyword(s):  
Molecular Marker ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Urothelial Carcinomas
Sign in / Sign up

Export Citation Format

Share Document