scholarly journals Allosteric modulation of peroxisomal membrane protein recognition by farnesylation of the peroxisomal import receptor PEX19

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Leonidas Emmanouilidis ◽  
Ulrike Schütz ◽  
Konstantinos Tripsianes ◽  
Tobias Madl ◽  
Juliane Radke ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Allosteric Modulation ◽  
Protein Recognition ◽  
Peroxisomal Membrane ◽  
Peroxisomal Membrane Protein ◽  
Import Receptor

Farnesylation of Pex19p is essential for peroxisomal membrane protein recognition

2007 ◽  
Vol 2007 (Spring) ◽  
Author(s):  
Robert Rucktäschel ◽  
Sven Thoms ◽  
Kirill Alexandrov ◽  
André Halbach ◽  
Markos Pechlivanis ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Protein Recognition ◽  
Peroxisomal Membrane ◽  
Peroxisomal Membrane Protein

scholarly journals Hydrophobic Regions Adjacent to Transmembrane Domains 1 and 5 Are Important for the Targeting of the 70-kDa Peroxisomal Membrane Protein

2007 ◽  
Vol 282 (46) ◽  
pp. 33831-33844 ◽  
Author(s):  
Yoshinori Kashiwayama ◽  
Kota Asahina ◽  
Masashi Morita ◽  
Tsuneo Imanaka
Keyword(s):  
Amino Acid ◽  
Membrane Protein ◽  
Protein Targeting ◽  
Fluorescent Protein ◽  
Chinese Hamster Ovary Cells ◽  
Intracellular Localization ◽  
Chinese Hamster ◽  
Transmembrane Domains ◽  
Peroxisomal Membrane ◽  
Peroxisomal Membrane Protein

The 70-kDa peroxisomal membrane protein (PMP70) is a major component of peroxisomal membranes. Human PMP70 consists of 659 amino acid residues and has six putative transmembrane domains (TMDs). PMP70 is synthesized on cytoplasmic ribosomes and targeted posttranslationally to peroxisomes by an unidentified peroxisomal membrane protein targeting signal (mPTS). In this study, to examine the mPTS within PMP70 precisely, we expressed various COOH-terminally or NH2-terminally deleted constructs of PMP70 fused with green fluorescent protein (GFP) in Chinese hamster ovary cells and determined their intracellular localization by immunofluorescence. In the COOH-terminally truncated PMP70, PMP70(AA.1-144)-GFP, including TMD1 and TMD2 of PMP70, was still localized to peroxisomes. However, by further removal of TMD2, PMP70(AA.1-124)-GFP lost the targeting ability, and PMP70(TMD2)-GFP did not target to peroxisomes by itself. The substitution of TMD2 in PMP70(AA.1-144)-GFP for TMD4 or TMD6 did not affect the peroxisomal localization, suggesting that PMP70(AA.1-124) contains the mPTS and an additional TMD is required for the insertion into the peroxisomal membrane. In the NH2-terminal 124-amino acid region, PMP70 possesses hydrophobic segments in the region adjacent to TMD1. By the disruption of these hydrophobic motifs by the mutation of L21Q/L22Q/L23Q or I70N/L71Q, PMP70(AA.1-144)-GFP lost targeting efficiency. The NH2-terminally truncated PMP70, GFP-PMP70(AA.263-375), including TMD5 and TMD6, exhibited the peroxisomal localization. PMP70(AA.263-375) also possesses hydrophobic residues (Ile307/Leu308) in the region adjacent to TMD5, which were important for targeting. These results suggest that PMP70 possesses two distinct targeting signals, and hydrophobic regions adjacent to the first TMD of each region are important for targeting.

scholarly journals Structural basis for docking of peroxisomal membrane protein carrier Pex19p onto its receptor Pex3p

2010 ◽  
Vol 29 (24) ◽  
pp. 4083-4093 ◽  
Author(s):  
Yasuhiko Sato ◽  
Hiroyuki Shibata ◽  
Toru Nakatsu ◽  
Hiroaki Nakano ◽  
Yoshinori Kashiwayama ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Structural Basis ◽  
Peroxisomal Membrane ◽  
Protein Carrier ◽  
Peroxisomal Membrane Protein

scholarly journals Proteasome-dependent protein quality control of the peroxisomal membrane protein Pxa1p

2020 ◽  
Vol 1862 (9) ◽  
pp. 183342
Author(s):  
S. Devarajan ◽  
M. Meurer ◽  
C.W.T. van Roermund ◽  
X. Chen ◽  
E.H. Hettema ◽  
...  
Keyword(s):  
Quality Control ◽  
Membrane Protein ◽  
Protein Quality ◽  
Protein Quality Control ◽  
Peroxisomal Membrane ◽  
Peroxisomal Membrane Protein ◽  
Dependent Protein
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