scholarly journals Tetrapod limb and sarcopterygian fin regeneration share a core genetic programme

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Acacio F. Nogueira ◽  
Carinne M. Costa ◽  
Jamily Lorena ◽  
Rodrigo N. Moreira ◽  
Gabriela N. Frota-Lima ◽  
...  
Keyword(s):  
Fin Regeneration ◽  
Genetic Programme

scholarly journals Limited dedifferentiation provides replacement tissue during zebrafish fin regeneration

2012 ◽  
Vol 365 (2) ◽  
pp. 339-349 ◽  
Author(s):  
Scott Stewart ◽  
Kryn Stankunas
Keyword(s):  
Fin Regeneration

scholarly journals Aryl Hydrocarbon Receptor Activation Impairs Extracellular Matrix Remodeling during Zebra Fish fin Regeneration

2006 ◽  
Vol 95 (1) ◽  
pp. 215-226 ◽  
Author(s):  
Eric A. Andreasen ◽  
Lijoy K. Mathew ◽  
Christiane V. Löhr ◽  
Rachelle Hasson ◽  
Robert L. Tanguay
Keyword(s):  
Extracellular Matrix ◽  
Aryl Hydrocarbon Receptor ◽  
Receptor Activation ◽  
Extracellular Matrix Remodeling ◽  
Matrix Remodeling ◽  
Zebra Fish ◽  
Fin Regeneration ◽  
Aryl Hydrocarbon

A proliferation gradient between proximal and msxb-expressing distal blastema directs zebrafish fin regeneration

Development ◽  
2002 ◽  
Vol 129 (11) ◽  
pp. 2607-2617 ◽  
Author(s):  
Alex Nechiporuk ◽  
Mark T. Keating
Keyword(s):  
High Rate ◽  
Proliferating Cells ◽  
Homogeneous Population ◽  
Fin Regeneration ◽  
New Model ◽  
Blastema Formation ◽  
Phosphohistone H3 ◽  
Slow Cycling ◽  
Early Regeneration ◽  
Regeneration Blastema

Previous studies of zebrafish fin regeneration led to the notion that the regeneration blastema is a homogeneous population of proliferating cells. Here, we show that the blastema consists of two components with markedly distinct proliferation properties. During early blastema formation, proliferating cells are evenly distributed. At the onset of regenerative outgrowth, however, blastemal cells are partitioned into two domains. Proximal blastemal cells proliferate at a high rate, shifting from a median G2 of more than 6 hours to approximately 1 hour. By contrast, the most distal blastemal cells do not proliferate. There is a gradient of proliferation between these extremes. Using bromodeoxyuridine incorporation and anti-phosphohistone H3 labeling, we find a 50-fold difference in proliferation across the gradient that extends approximately 50 μm, or ten cell diameters. We show that during early regeneration, proliferating blastemal cells express msxb, a homeodomain transcriptional repressor. While msxb is widely expressed among proliferating cells during blastema formation, its expression becomes restricted to a small number of non-proliferating, distal blastemal cells during regenerative outgrowth. Bromodeoxyuridine pulse-chase experiments show that distal and proximal blastemal cells are formed from proliferating, msxb-positive blastemal cells, not from preexisting slow-cycling cells. These data support the idea that blastema formation results from dedifferentiation of intraray mesenchymal cells. Based on these findings, we propose a new model of zebrafish fin regeneration in which the function of non-proliferating, msxb-expressing, distal blastemal cells is to specify the boundary of proliferation and provide direction for regenerative outgrowth.

scholarly journals Two Different Transgenes to Study Gene Silencing and Re-Expression During Zebrafish Caudal Fin and Retinal Regeneration

2006 ◽  
Vol 6 ◽  
pp. 65-81 ◽  
Author(s):  
Ryan Thummel ◽  
Christopher T. Burket ◽  
David R. Hyde
Keyword(s):  
Tissue Regeneration ◽  
Transgene Expression ◽  
Egfp Expression ◽  
Transgenic Zebrafish ◽  
Retinal Regeneration ◽  
Caudal Fin ◽  
Fin Regeneration ◽  
Differentiated Cells ◽  
Neuronal Progenitors ◽  
Wound Epithelium

We used the 500-bpXenopusef1-α promoter and the 2-kb zebrafish histone2A.F/Zpromoter to generate several independent transgenic zebrafish lines expressing EGFP. While both promoters drive ubiquitous EGFP expression in early zebrafish development, they are systematically silenced in several adult tissues, including the retina and caudal fin. However, EGFP expression is temporarily renewed in the adult during either caudal fin or retinal regeneration. In the Tg(H2A.F/Z:EGFP)ntline, EGFP is moderately expressed in both the wound epithelium and blastema of the regenerating caudal fin. In the Tg(ef1-α:EGFP)ntline, EGFP expression is reinitiated and restricted to the blastema of the regenerating caudal fin and colabels with BrdU, PCNA, andmsxc-positive cells. Thus, these two ubiquitous promoters drive EGFP transgene expression in different cell populations during caudal fin regeneration. We further analyzed the ability of theef1-α:EGFPtransgene to label nonterminally differentiated cells during adult tissue regeneration. First, we demonstrated that the transgene is highly methylated in adult zebrafish caudal fin tissue, but not during fin regeneration, implicating methylation as a potential means of transgene silencing in this line. Next, we determined that theef1-α:EGFPtransgene is also re-expressed during adult retinal regeneration. Specifically, theef1-α:EGFPtransgene colabels with PCNA in the Müglia, a specialized cell that is the source of neuronal progenitors during zebrafish retinal regeneration. Thus, we concluded that Tg(ef1-α:EGFP)nt line visually marks nonterminally differentiated cells in multiple adult regeneration environments and may prove to be a useful marker in tissue regeneration studies in zebrafish.

Histochemically defined cell states during tail fin regeneration in teleost fishes

1996 ◽  
Vol 60 (3) ◽  
pp. 139-149 ◽  
Author(s):  
M. Marí-Beffa ◽  
J.A. Santamaría ◽  
P. Fernández-Llebrez ◽  
J. Becerra
Keyword(s):  
Teleost Fishes ◽  
Fin Regeneration

scholarly journals Ray–Interray Interactions during Fin Regeneration of Danio rerio

2002 ◽  
Vol 252 (2) ◽  
pp. 214-224 ◽  
Author(s):  
C. Murciano ◽  
T.D. Fernández ◽  
I. Durán ◽  
D. Maseda ◽  
J. Ruiz-Sánchez ◽  
...  
Keyword(s):  
Danio Rerio ◽  
Fin Regeneration

Vitamin C supplementation improves growth performance and caudal fin regeneration in zebrafish ( Danio rerio )

2021 ◽  
Author(s):  
Pedro Luiz Pucci Figueiredo Carvalho ◽  
Pedro Henrique Ventura Almeida ◽  
William dos Santos Xavier ◽  
Igor Simões Tiagua Vicente ◽  
Matheus Gardim Guimarães ◽  
...  
Keyword(s):  
Vitamin C ◽  
Growth Performance ◽  
Danio Rerio ◽  
Caudal Fin ◽  
Fin Regeneration ◽  
Vitamin C Supplementation
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