scholarly journals Mohawk promotes the maintenance and regeneration of the outer annulus fibrosus of intervertebral discs

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Ryo Nakamichi ◽  
Yoshiaki Ito ◽  
Masafumi Inui ◽  
Naoko Onizuka ◽  
Tomohiro Kayama ◽  
...  
Keyword(s):  
Annulus Fibrosus ◽  
Intervertebral Discs ◽  
Outer Annulus ◽  
Outer Annulus Fibrosus

scholarly journals Generation of an in vitro model of the outer annulus fibrosus‐cartilage interface

JOR Spine ◽  
2020 ◽  
Vol 3 (2) ◽  
Author(s):  
Jasmine E. Chong ◽  
J. Paul Santerre ◽  
Rita A. Kandel
Keyword(s):  
Annulus Fibrosus ◽  
In Vitro Model ◽  
Outer Annulus ◽  
Vitro Model ◽  
Outer Annulus Fibrosus

Effects of Swelling Conditions on the Compressive Properties of Nucleus Pulposus From Bovine Intervertebral Discs

2004 ◽  
Author(s):  
David T. Korda ◽  
Delphine Perie ◽  
James C. Iatridis
Keyword(s):  
Intervertebral Disc ◽  
Water Content ◽  
Nucleus Pulposus ◽  
Annulus Fibrosus ◽  
Compressive Loading ◽  
Collagen Matrix ◽  
High Water ◽  
Intervertebral Discs ◽  
High Water Content ◽  
Outer Annulus Fibrosus

The intervertebral disc provides flexibility and load support for the spine. It consists of two main regions; the outer annulus fibrosus which is a highly organized collagen matrix and the inner nucleus pulposus which (in a healthy disc) is a proteoglycan rich gelatinous material. The predominant mode of loading on the intervertebral disc is axial compression, which generates hydrostatic pressures within the disc. The high water content of the nucleus plays a major role in supporting these loads. With age and degeneration, the water content of the nucleus changes, and is believed to significantly impact its ability to bear load. The purpose of this study therefore, was to define the effects of swelling conditions (which affect disc hydration) on the material properties of the disc under compressive loading.

In situ intercellular mechanics of the bovine outer annulus fibrosus subjected to biaxial strains

2004 ◽  
Vol 37 (2) ◽  
pp. 223-231 ◽  
Author(s):  
Sabina B. Bruehlmann ◽  
Paul A. Hulme ◽  
Neil A. Duncan
Keyword(s):  
Annulus Fibrosus ◽  
Outer Annulus ◽  
Outer Annulus Fibrosus

Dynamic Hydrostatic Pressurization Differentially Regulates Extracellular Matrix Elaboration by Bovine Inner and Outer Annulus Fibrosus Cells Seeded on 3-D Polymer Scaffolds

2007 ◽  
Author(s):  
Anna T. Reza ◽  
Steven B. Nicoll
Keyword(s):  
Extracellular Matrix ◽  
Mechanical Loading ◽  
Annulus Fibrosus ◽  
Type Ii Collagen ◽  
Culture Conditions ◽  
Phenotypic Properties ◽  
Outer Annulus ◽  
The Impact ◽  
Outer Annulus Fibrosus

Current surgical treatments for intervertebral disc (IVD) degeneration result in decreased mobility of the spine [1]. A tissue engineering approach may provide an alternative that restores both IVD structure and function. The IVD is comprised of three distinct regions: the outer annulus fibrosus (OA), inner annulus fibrosus (IA), and the nucleus pulposus (NP). Each of the cell populations within these regions possess unique phenotypic properties that are greatly influenced by environmental factors, such as the surrounding 3-D extracellular matrix (ECM) and mechanical loading (i.e., hydrostatic pressurization) [2]. As such, both the 3-D scaffold and in vitro culture conditions may have marked effects on the development of tissue-engineered IVD constructs. Although the influence of mechanical loading on IVD cells and explants has been investigated, no prior studies have examined the impact of hydrostatic pressurization on OA and IA cells in 3-D culture. Therefore, the objective of this study was to determine the effects of dynamic hydrostatic pressurization on OA and IA cells seeded on 3-D fibrous poly(glycolic acid)-poly(L-lactic acid) (PGA-PLLA) scaffolds. We hypothesized that the application of hydrostatic pressure would promote increased production of type II collagen and chondroitin sulfate proteoglycan in both OA- and IA-seeded constructs.

scholarly journals Transcriptional profiling distinguishes inner and outer annulus fibrosus from nucleus pulposus in the bovine intervertebral disc

2017 ◽  
Vol 26 (8) ◽  
pp. 2053-2062 ◽  
Author(s):  
Guus G. H. van den Akker ◽  
Marije I. Koenders ◽  
Fons A. J. van de Loo ◽  
Peter L. E. M. van Lent ◽  
Esmeralda Blaney Davidson ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Nucleus Pulposus ◽  
Annulus Fibrosus ◽  
Transcriptional Profiling ◽  
Outer Annulus ◽  
Outer Annulus Fibrosus

Micro-Scale Strain Transfer in Fiber-Reinforced Native Tissue Is Distinct From Cell-Seeded Aligned Nanofibrous Scaffolds

2012 ◽  
Author(s):  
Woojin M. Han ◽  
Su-Jin Heo ◽  
Tristan P. Driscoll ◽  
Robert L. Mauck ◽  
Dawn M. Elliott
Keyword(s):  
Extracellular Matrix ◽  
Cell Viability ◽  
Annulus Fibrosus ◽  
Tissue Level ◽  
Strain Fields ◽  
Strain Transfer ◽  
Micro Scale ◽  
Nanofibrous Scaffolds ◽  
Outer Annulus ◽  
Outer Annulus Fibrosus

Mechanical signals influence cell viability, differentiation, proliferation, and extracellular matrix (ECM) production in load-bearing tissues. However, the current understanding of how macroscopic tissue level strain is transferred to cells is confounded by the highly variable strain fields that arise within the ECM of these tissues. In tendon and outer annulus fibrosus (AF), microscale strains in the ECM can be significantly lower than the applied strains.1,2,3 In meniscus, both strain amplification and attenuation were observed at the microscale level.4

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