scholarly journals Laser capture microscopy coupled with Smart-seq2 for precise spatial transcriptomic profiling

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Susanne Nichterwitz ◽  
Geng Chen ◽  
Julio Aguila Benitez ◽  
Marlene Yilmaz ◽  
Helena Storvall ◽  
...  
Keyword(s):  
Transcriptomic Profiling ◽  
Laser Capture Microscopy ◽  
Laser Capture

scholarly journals The Use of Laser Capture Microscopy in Proteomics Research – A Review

2004 ◽  
Vol 20 (3) ◽  
pp. 155-160 ◽  
Author(s):  
George M. Kunz ◽  
Daniel W. Chan
Keyword(s):  
Laser Capture Microscopy ◽  
Proteomics Research ◽  
Laser Capture

Cambridge Healthtech Institute’s 3rd Annual Laser Capture Microscopy: Cutting Edge Applications

2005 ◽  
Vol 5 (4) ◽  
pp. 487-491 ◽  
Author(s):  
Vasker Bhattacherjee ◽  
Robert Greene ◽  
M Michele Pisano
Keyword(s):  
Cutting Edge ◽  
Laser Capture Microscopy ◽  
Laser Capture

Laser capture microscopy as a tool for the assessment of lineage-specific chimaerism from archived blood and bone marrow films

2007 ◽  
Vol 136 (4) ◽  
pp. 677-678 ◽  
Author(s):  
Julie Richards ◽  
G. Yvonne Morgan ◽  
Laurence Pearce ◽  
Piers E. M. Patten ◽  
Ghulam J. Mufti ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Laser Capture Microscopy ◽  
Laser Capture

scholarly journals Human Neutrophil Peptides 1-3 – Early Markers in Development of Colorectal Adenomas and Carcinomas

2008 ◽  
Vol 25 (2) ◽  
pp. 123-129 ◽  
Author(s):  
Henning Mothes ◽  
Christian Melle ◽  
Günther Ernst ◽  
Roland Kaufmann ◽  
Ferdinand von Eggeling ◽  
...  
Keyword(s):  
Human Neutrophil ◽  
Tumour Growth ◽  
Tumour Response ◽  
Colorectal Adenomas ◽  
Cancer Stage ◽  
Tumour Stage ◽  
Laser Capture Microscopy ◽  
Early Markers ◽  
Laser Capture

Expression of Human Neutrophil Peptides (HNP) 1–3 was recently found to be associated with development of colorectal cancer. Raised defensin-expression in tumours is believed to stem from increased infiltration of neutrophils into tumour environment.To further specify the role of α-defensins in tumourigenesis and progression, HNP1–3 were analyzed in colorectal adenomas and carcinomas of 87 patients and quantified in relation to cancer stage and grading. Using the ProteinChip arrays, HNP1–3 were found upregulated in both colorectal adenomas and carcinomas. By combining the array with Laser capture microscopy we were able to confirm that HNP1–3 are expressed by tumour cells but not by neutrophils or other tumour invading cells. These findings suggest that α-defensins are more likely to contribute to tumour growth than they are to mount an effective host anti-tumour response. However, the amount of HNP-expression was not found to be related to tumour stage, grading, and serological tumour markers.

scholarly journals Time Course Gene Expression Using Laser Capture Microscopy-Extracted Bile Ducts, but Not Hepatic Parenchyma, Reveals Acute Alpha-Naphthylisothiocyanate Toxicity

2010 ◽  
Vol 38 (5) ◽  
pp. 715-729 ◽  
Author(s):  
John Michael Cullen ◽  
James Greg Falls ◽  
Harlan Roger Brown ◽  
Lawrence Wonsik Yoon ◽  
Neal Foster Cariello ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bile Ducts ◽  
Time Course ◽  
Hepatic Parenchyma ◽  
Laser Capture Microscopy ◽  
Laser Capture

Layer-specific differences of gene expression in extraocular muscles identified by laser-capture microscopy

2004 ◽  
Vol 20 (1) ◽  
pp. 55-65 ◽  
Author(s):  
Murat T. Budak ◽  
Sasha Bogdanovich ◽  
Martin H. J. Wiesen ◽  
Olga Lozynska ◽  
Tejvir S. Khurana ◽  
...  
Keyword(s):  
Metabolic Pathways ◽  
Extraocular Muscles ◽  
Molecular Evidence ◽  
Structural Elements ◽  
Evolutionary Mechanisms ◽  
Functional Roles ◽  
Laser Capture Microscopy ◽  
Vascular Elements ◽  
Genes Encoding ◽  
Laser Capture

In mammals, separate muscles are typically specialized as a whole to provide distinct functional roles leading to well-recognized adaptations. This is exemplified in the lower limb by the slow, fatigue-resistant soleus, which provides a postural role vs. the fast, fatiguable tibialis anterior (TA), which provides rapid movements. A unique characteristic of extraocular muscles (EOMs) is their compartmentalization into two distinct layers, the orbital layer (OL) and global layer (GL), presumably to subserve diverse functions within the same muscle. However, molecular evidence of this diversity has been limited. We used laser-capture microscopy coupled with microarray-based expression profiling to identify molecular differences between the OL and GL of rat EOMs. We found that 210 genes were differentially regulated between these layers at a twofold expression cutoff. Differences in genes related to metabolic pathways and related to structural elements of muscle and nerve formed the largest functional clusters. Layer-specific differential expression was validated at both mRNA and protein level for MYH3, MYH6, and ACTN3. The expected layer-specific differences among genes encoding vascular elements were not evident by profiling; morphometric analysis demonstrated that the differences exist, but at a magnitude below the cutoff level established by our statistical methods. Comparison of these results with previous results comparing whole EOMs and TA suggest evolutionary mechanisms may play a role in achieving functional distinctions between OL and GL.

scholarly journals Low-Coverage Whole Genome Sequencing Using Laser Capture Microscopy with Combined Digital Droplet PCR: An Effective Tool to Study Copy Number and Kras Mutations in Early Lung Adenocarcinoma Development

2021 ◽  
Vol 22 (21) ◽  
pp. 12034
Author(s):  
Elizabeth A. Mickler ◽  
Huaxin Zhou ◽  
Tzu L. Phang ◽  
Mark W. Geraci ◽  
Robert S. Stearman ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Genome Sequencing ◽  
Copy Number ◽  
Whole Genome ◽  
Kras Mutations ◽  
Digital Droplet Pcr ◽  
Laser Capture Microscopy ◽  
Droplet Pcr ◽  
Low Coverage ◽  
Laser Capture

Defining detailed genomic characterization of early tumor progression is critical to identifying key regulators and pathways in carcinogenesis as potentially druggable targets. In human lung cancer, work to characterize early cancer development has mainly focused on squamous cancer, as the earliest lesions are more proximal in the airways and often accessible by repeated bronchoscopy. Adenocarcinomas are typically located distally in the lung, limiting accessibility for biopsy of pre-malignant and early stages. Mouse lung cancer models recapitulate many human genomic features and provide a model for tumorigenesis with pre-malignant atypical adenomatous hyperplasia and in situ adenocarcinomas often developing contemporaneously within the same animal. Here, we combined tissue characterization and collection by laser capture microscopy (LCM) with digital droplet PCR (ddPCR) and low-coverage whole genome sequencing (LC-WGS). ddPCR can be used to identify specific missense mutations in Kras (Kirsten rat sarcoma viral oncogene homolog, here focused on Kras Q61) and estimate the percentage of mutation predominance. LC-WGS is a cost-effective method to infer localized copy number alterations (CNAs) across the genome using low-input DNA. Combining these methods, the histological stage of lung cancer can be correlated with appearance of Kras mutations and CNAs. The utility of this approach is adaptable to other mouse models of human cancer.

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