scholarly journals F-actin mechanics control spindle centring in the mouse zygote

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Agathe Chaigne ◽  
Clément Campillo ◽  
Raphaël Voituriez ◽  
Nir S. Gov ◽  
Cécile Sykes ◽  
...  
Keyword(s):  
Mouse Zygote

Regulation of fertilization-induced Ca2+spiking in the mouse zygote

Cell Calcium ◽  
2000 ◽  
Vol 28 (1) ◽  
pp. 47-54 ◽  
Author(s):  
M.L. Day ◽  
O.M. McGuinness ◽  
M.J. Berridge ◽  
M.H. Johnson
Keyword(s):  
Mouse Zygote

Sperm entry position provides a surface marker for the first cleavage plane of the mouse zygote

genesis ◽  
2002 ◽  
Vol 32 (3) ◽  
pp. 193-198 ◽  
Author(s):  
Berenika Plusa ◽  
Karolina Piotrowska ◽  
Magdalena Zernicka-Goetz
Keyword(s):  
Cleavage Plane ◽  
Surface Marker ◽  
Sperm Entry ◽  
Mouse Zygote

338. METHYL BINDING DOMAIN PROTEIN (MBD1) IN THE NUCLEUS OF THE MOUSE ZYGOTE

2010 ◽  
Vol 22 (9) ◽  
pp. 138
Author(s):  
Y. Li ◽  
X. L. Jin ◽  
C. O'Neill
Keyword(s):  
Cell Cycle ◽  
Acid Treatment ◽  
Metaphase Chromosomes ◽  
Methylated Dna ◽  
Stage Of Development ◽  
Genome Methylation ◽  
Mouse Zygote ◽  
Domain Protein ◽  
Proxy Measurement ◽  
Current Paradigm

MBD1 is one of five proteins which bind methylated DNA and regulate gene transcription. The binding of these proteins, particularly MBD1, is commonly used as a proxy measurement of global CpG methylation. Since methylation is reported to be highly dynamic during the first cell-cycle, with reported asymmetric global demethylation of the paternal and maternal genomes by the time of syngamy, we were interested to assess the pattern of staining of the MBD1 during this stage of development. A specific antibody to MBD1 was shown by Western analysis to detect in zygotes a protein of predicted mass. Using immunolocalization, however, we found no staining in pronuclei. Brief acid treatment (10min, 4M HCl) followed by immunolabelling revealed strong pronuclear MBD1 staining throughout the maturation of the zygote and on metaphase chromosomes, indicative of epitope masking under normal staining conditions. Upon unmasking by acid treatment zygotes collected fresh from the oviduct did not show consistent differences in MBD1 staining between the maternal or paternal chromosomes or pronuclei, but for those embryos produced by IVF we found more MBD1 staining in the male paternal pronucleus. Brief treatment with trypsin caused a marked loss of MBD1 staining and this treatment increased the extent of staining of 5-methylcytosine. These results show that MBD1 antigen persists on DNA after treatments normally used for the detection of 5-methylcytosine. MBD1 at least partially masks methylcytosine from immunological detection and the results therefore raise the possibility that the reported changes in genome methylation in the zygote are a consequence of the binding of MBD1. If MBD1 binding is truly a proxy for methylation, the persistence of high levels of MBD1 throughout the first cell-cycle questions the current paradigm of global demethylation during zygote maturation.

MEMS Nanoinjector for Injecting Foreign DNA Into Living Cells

2009 ◽  
Author(s):  
Quentin Aten
Keyword(s):  
Genetic Material ◽  
Living Cells ◽  
Harsh Environment ◽  
Electron Micrograph ◽  
Scanning Electron Micrograph ◽  
Mouse Zygote ◽  
Foreign Dna ◽  
Mems Device ◽  
Mouse Egg ◽  
Better Than

The nanoinjector is a MEMS device that has been successfully used to inject foreign genetic material into fertilized mouse egg cells (zygotes). This scanning electron micrograph shows a nanoinjector grasping a 100 μm diameter latex sphere. The sphere is roughly the size of a mouse zygote, and it can withstand the harsh environment in the electron microscope better than a mouse zygote. The nanoinjector’s two constraining mechanisms (at left and top-right) and lance mechanism (bottom right) are fabricated from two planar layers of polysilicon through MEMSCAP’s polysilicon Multi-User MEMS Processes (polyMUMPs)

scholarly journals Hypomethylation of paternal DNA in the late mouse zygote is not essential for development

2008 ◽  
Vol 52 (2-3) ◽  
pp. 295-298 ◽  
Author(s):  
Zbigniew Polanski ◽  
Nami Motosugi ◽  
Chizuko Tsurumi ◽  
Takashi Hiiragi ◽  
Steffen Hoffmann
Keyword(s):  
Mouse Zygote

scholarly journals Determining the first cleavage of the mouse zygote

2003 ◽  
Vol 6 (2) ◽  
pp. 160-163 ◽  
Author(s):  
Magdalena Zernicka-Goetz
Keyword(s):  
Mouse Zygote

scholarly journals DNA barcoding reveals that injected transgenes are predominantly processed by homologous recombination in mouse zygote

2019 ◽  
Author(s):  
Alexander Smirnov ◽  
Anastasia Yunusova ◽  
Alexey Korablev ◽  
Irina Serova ◽  
Veniamin Fishman ◽  
...  
Keyword(s):  
Homologous Recombination ◽  
Dna Molecules ◽  
Dna Breaks ◽  
End Joining ◽  
Exogenous Dna ◽  
Transgenic Organisms ◽  
Mouse Zygote ◽  
Non Homologous End Joining ◽  
Transgenic Embryos ◽  
Double Holliday Junction

AbstractMechanisms that ensure repair of double-stranded DNA breaks play a key role in the integration of foreign DNA into the genome of transgenic organisms. After pronuclear microinjection, exogenous DNA is usually found in the form of concatemer consisting of multiple co-integrated transgene copies. Here we investigated contribution of various DSB repair pathways to the concatemer formation. We injected a pool of linear DNA molecules carrying unique barcodes at both ends into mouse zygotes and obtained 10 transgenic embryos with transgene copy number ranging from 1 to 300 copies. Sequencing of the barcodes allowed us to assign relative positions to the copies in concatemers and to detect recombination events that happened during integration. Cumulative analysis of approximately 1000 integrated copies revealed that more than 80% of copies underwent recombination when their linear ends were processed by SDSA or DSBR. We also observed evidence of double Holliday junction (dHJ) formation and crossing-over during the formation of concatemers. Additionally, sequencing of indels between copies showed that at least 10% of the DNA molecules introduced into the zygote are ligated by non-homologous end joining (NHEJ). Our barcoding approach documents high activity of homologous recombination after exogenous DNA injection in mouse zygote.

The first cleavage of the mouse zygote predicts the blastocyst axis

Nature ◽  
2005 ◽  
Vol 434 (7031) ◽  
pp. 391-395 ◽  
Author(s):  
Berenika Plusa ◽  
Anna-Katerina Hadjantonakis ◽  
Dionne Gray ◽  
Karolina Piotrowska-Nitsche ◽  
Agnieszka Jedrusik ◽  
...  
Keyword(s):  
Mouse Zygote
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