scholarly journals Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Patrick Guye ◽  
Mohammad R. Ebrahimkhani ◽  
Nathan Kipniss ◽  
Jeremy J. Velazquez ◽  
Eldi Schoenfeld ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Self Organization

scholarly journals Self-Organization of Polarized Cerebellar Tissue in 3D Culture of Human Pluripotent Stem Cells

Cell Reports ◽  
2015 ◽  
Vol 10 (4) ◽  
pp. 537-550 ◽  
Author(s):  
Keiko Muguruma ◽  
Ayaka Nishiyama ◽  
Hideshi Kawakami ◽  
Kouichi Hashimoto ◽  
Yoshiki Sasai
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
3D Culture ◽  
Human Pluripotent Stem Cells ◽  
Self Organization ◽  
Cerebellar Tissue

scholarly journals Optogenetic control of Wnt signaling for modeling early embryogenic patterning with human pluripotent stem cells

2019 ◽  
Author(s):  
Nicole A. Repina ◽  
Xiaoping Bao ◽  
Joshua A. Zimmermann ◽  
David A. Joy ◽  
Ravi S. Kane ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wnt Signaling ◽  
Pluripotent Stem Cells ◽  
High Efficiency ◽  
Dynamic Range ◽  
Embryonic Stem ◽  
Human Pluripotent Stem Cells ◽  
Self Organization ◽  
Human Escs ◽  
Mesenchymal Transition

ABSTRACTThe processes of cell proliferation, differentiation, migration, and self-organization during early embryonic development are governed by dynamic, spatially and temporally varying morphogen signals. Analogous tissue patterns emerge spontaneously in embryonic stem cell (ESC) models for gastrulation, but mechanistic insight into this self-organization is limited by a lack of molecular methods to precisely control morphogen signal dynamics. Here we combine optogenetic stimulation and single-cell imaging approaches to study self-organization of human pluripotent stem cells. Precise control of morphogen signal dynamics, achieved through activation of canonical Wnt/β-catenin signaling over a broad high dynamic range (>500-fold) using an optoWnt optogenetic system, drove broad transcriptional changes and mesendoderm differentiation of human ESCs at high efficiency (>95% cells). Furthermore, activating Wnt signaling in subpopulations of ESCs in 2D and 3D cultures induced cell self-organization and morphogenesis reminiscent of human gastrulation, including changes in cell migration and epithelial to mesenchymal transition. Our findings thus reveal an instructive role for Wnt in directing cell patterning in this ESC model for gastrulation.

Differentiation of human pluripotent stem cells into airway epithelial cells

Pneumologie ◽  
2015 ◽  
Vol 69 (07) ◽  
Author(s):  
S Ulrich ◽  
S Weinreich ◽  
R Haller ◽  
S Menke ◽  
R Olmer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Epithelial Cells ◽  
Pluripotent Stem Cells ◽  
Airway Epithelial Cells ◽  
Human Pluripotent Stem Cells ◽  
Airway Epithelial

326-LB: BCL-xL/BCL2L1 Is a Critical Anti-Apoptotic Protein that Suppresses BAK to Promote Pancreatic Specification from Human Pluripotent Stem Cells

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 326-LB
Author(s):  
LARRY SAI WENG LOO ◽  
ADRIAN TEO ◽  
SOUMITA GHOSH ◽  
ANDREAS ALVIN PURNOMO SOETEDJO ◽  
LINH NGUYEN ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Apoptotic Protein
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