scholarly journals Structure–function characterization reveals new catalytic diversity in the galactose oxidase and glyoxal oxidase family

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
DeLu Yin ◽  
Saioa Urresti ◽  
Mickael Lafond ◽  
Esther M. Johnston ◽  
Fatemeh Derikvand ◽  
...  
Keyword(s):  
Structure Function ◽  
Galactose Oxidase ◽  
Function Characterization ◽  
Glyoxal Oxidase

Structure-function characterization reveals catalytic diversity in the galactose oxidase family

2016 ◽  
Vol 72 (a1) ◽  
pp. s33-s34
Author(s):  
Saioa Urresti ◽  
DeLu T. Yin ◽  
Mickael Lafond ◽  
Esther M. Johnston ◽  
Fatemeh Derikvand ◽  
...  
Keyword(s):  
Structure Function ◽  
Galactose Oxidase ◽  
Function Characterization

scholarly journals Comprehensive structure-function characterization of DNMT3B and DNMT3A reveals distinctive de novo DNA methylation mechanisms

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Linfeng Gao ◽  
Max Emperle ◽  
Yiran Guo ◽  
Sara A. Grimm ◽  
Wendan Ren ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Structure Function ◽  
De Novo ◽  
Function Characterization

scholarly journals Salt-free fractionation of complex isomeric mixtures of glycosaminoglycan oligosaccharides compatible with ESI-MS and microarray analysis

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Hao Liu ◽  
Apoorva Joshi ◽  
Pradeep Chopra ◽  
Lin Liu ◽  
Geert-Jan Boons ◽  
...  
Keyword(s):  
Structure Function ◽  
Structural Complexity ◽  
Reversed Phase ◽  
Separation Method ◽  
Size Exclusion ◽  
Reaction Products ◽  
Exclusion Chromatography ◽  
Function Characterization ◽  
Function Relationship ◽  
Isomeric Mixtures

Abstract Heparin and heparan sulfate (Hp/HS) are linear complex glycosaminoglycans which are involved in diverse biological processes. The structural complexity brings difficulties in separation, making the study of structure-function relationships challenging. Here we present a separation method for Hp/HS oligosaccharide fractionation with cross-compatible solvent and conditions, combining size exclusion chromatography (SEC), ion-pair reversed phase chromatography (IPRP), and hydrophilic interaction chromatography (HILIC) as three orthogonal separation methods that do not require desalting or extensive sample handling. With this method, the final eluent is suitable for structure-function relationship studies, including tandem mass spectrometry and microarray printing. Our data indicate that high resolution is achieved on both IPRP and HILIC for Hp/HS isomers. In addition, the fractions co-eluted in IPRP could be further separated by HILIC, with both separation dimensions capable of resolving some isomeric oligosaccharides. We demonstrate this method using both unpurified reaction products from isomeric synthetic hexasaccharides and an octasaccharide fraction from enoxaparin, identifying isomers resolved by this multi-dimensional separation method. We demonstrate both structural analysis by MS, as well as functional analysis by microarray printing and screening using a prototypical Hp/HS binding protein: basic-fibroblast growth factor (FGF2). Collectively, this method provides a strategy for efficient Hp/HS structure-function characterization.

Structure–function characterization of the recombinant aspartic proteinase A1 from Arabidopsis thaliana

2010 ◽  
Vol 71 (5-6) ◽  
pp. 515-523 ◽  
Author(s):  
Miguel A. Mazorra-Manzano ◽  
Takuji Tanaka ◽  
Derek R. Dee ◽  
Rickey Y. Yada
Keyword(s):  
Arabidopsis Thaliana ◽  
Structure Function ◽  
Aspartic Proteinase ◽  
Function Characterization
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