Metalloproteinases in mammary stem cell activity

2013 ◽  
Vol 15 (9) ◽  
pp. 1033-1033
Author(s):  
Nathalie Le Bot
Keyword(s):  
Stem Cell ◽  
Cell Activity ◽  
Mammary Stem Cell ◽  
Stem Cell Activity ◽  
Mammary Stem

CD24 and CD49f expressions of E14.5 mouse mammary anlagen cells define putative distribution of earlier embryonic mammary stem cell activities

2018 ◽  
Vol 96 (5) ◽  
pp. 539-547 ◽  
Author(s):  
Jiazhe Song ◽  
Fangrong Ding ◽  
Song Li ◽  
Wenzhe Li ◽  
Ning Li ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mammary Gland ◽  
Cell Population ◽  
Cell Biology ◽  
Cell Activity ◽  
Mammary Stem Cell ◽  
Flow Cytometry Analysis ◽  
Large Numbers ◽  
Stem Cell Activity ◽  
Mammary Stem

Stem cell biology offers promise for understanding the origins of the mammary gland. However, the distribution of mammary stem cell (MaSC) activities at earlier embryonic stages has not been fully identified. The markers for sorting adult MaSC, CD24, CD29, and CD49f have been applied to analyze fetal MaSCs. Here we explored mammary anlagen MaSCs by investigating the expression of CD24 and CD49f. According to the comparative analysis between adult mammary gland and fetal mammary anlagen, we found that fetal mouse mammary anlagen may possess a high percentage of potential MaSCs. Flow cytometry analysis revealed 2 distinct mammary anlagen populations: Lin–CD24med and Lin–CD24high. Sphere-forming and mammary repopulating assays confirmed that the stem cell activity of E14.5 mouse mammary anlagen was restricted to the Lin–CD24med cell population. Furthermore, CD24med mammary anlagen cells were separated into Lin–CD24medCD49f+ and Lin–CD24medCD49f– populations and identified, respectively. The results proved that the mammary anlagen Lin–CD24medCD49f+ cell population possesses more stem cell activities than the Lin–CD24medCD49f– cell population. However, a limited numbers of stem cells and large numbers of stromal cells were identified in mammary anlagen in the Lin–CD24med cell population.

scholarly journals Dissociation of estrogen receptor expression and in vivo stem cell activity in the mammary gland

2006 ◽  
Vol 176 (1) ◽  
pp. 19-26 ◽  
Author(s):  
Katherine E. Sleeman ◽  
Howard Kendrick ◽  
David Robertson ◽  
Clare M. Isacke ◽  
Alan Ashworth ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Estrogen Receptor ◽  
Stem Cell ◽  
Epithelial Cell ◽  
Cell Activity ◽  
Mammary Stem Cell ◽  
Stem Cell Proliferation ◽  
Stem Cell Activity ◽  
Mammary Stem

The role of estrogen in promoting mammary stem cell proliferation remains controversial. It is unclear if estrogen receptor (ER)–expressing cells have stem/progenitor activity themselves or if they act in a paracrine fashion to stimulate stem cell proliferation. We have used flow cytometry to prospectively isolate mouse mammary ER-expressing epithelial cells and shown, using analysis of gene expression patterns and cell type–specific markers, that they form a distinct luminal epithelial cell subpopulation that expresses not only the ER but also the progesterone and prolactin receptors. Furthermore, we have used an in vivo functional transplantation assay to directly demonstrate that the ER-expressing luminal epithelial subpopulation contains little in vivo stem cell activity. Rather, the mammary stem cell activity is found within the basal mammary epithelial cell population. Therefore, ER-expressing cells of the mammary epithelium are distinct from the mammary stem cell population, and the effects of estrogen on mammary stem cells are likely to be mediated indirectly. These results are important for our understanding of cellular responses to hormonal stimulation in the normal breast and in breast cancer.

Abstract B136: Sox10 expression labels in vitro mammary stem cell activity

2013 ◽  
Author(s):  
Christopher Dravis ◽  
Claire Johns ◽  
Benjamin T. Spike ◽  
Michelle Southard Smith ◽  
Geoffrey M. Wahl
Keyword(s):  
Stem Cell ◽  
Cell Activity ◽  
Mammary Stem Cell ◽  
Stem Cell Activity ◽  
Mammary Stem ◽  
Sox10 Expression

scholarly journals Lgr5 is a marker for fetal mammary stem cells, but is not essential for stem cell activity or tumorigenesis

2017 ◽  
Vol 3 (1) ◽  
Author(s):  
Christy L. Trejo ◽  
Gidsela Luna ◽  
Christopher Dravis ◽  
Benjamin T. Spike ◽  
Geoffrey M. Wahl
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Activity ◽  
Mammary Stem Cells ◽  
Stem Cell Activity ◽  
Mammary Stem
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