scholarly journals Metabolic differentiation in the embryonic retina

2012 ◽  
Vol 14 (8) ◽  
pp. 859-864 ◽  
Author(s):  
Michalis Agathocleous ◽  
Nicola K. Love ◽  
Owen Randlett ◽  
Julia J. Harris ◽  
Jinyue Liu ◽  
...  
Keyword(s):  
Embryonic Retina

scholarly journals Zebrafish Radar: A new member of the TGF-β superfamily defines dorsal regions of the neural plate and the embryonic retina

1995 ◽  
Vol 49 (3) ◽  
pp. 223-234 ◽  
Author(s):  
Monique Rissi ◽  
Jochen Wittbrodt ◽  
Emmanuèle Délot ◽  
Martin Naegeli ◽  
Frédéric M. Rosa
Keyword(s):  
Neural Plate ◽  
Embryonic Retina

scholarly journals The microRNA miR-18a Links Proliferation and Inflammation During Photoreceptor Regeneration in the Injured Zebrafish Retina

2021 ◽  
Author(s):  
Evin Magner ◽  
Pamela Sandoval-Sanchez ◽  
Peter F Hitchcock ◽  
Scott M Taylor
Keyword(s):  
Muller Glia ◽  
Müller Glia ◽  
Wild Type ◽  
Post Injury ◽  
Photoreceptor Layer ◽  
Brdu Labeling ◽  
Key Aspects ◽  
The Brain ◽  
Embryonic Retina

Abstract In mammals, photoreceptor loss causes permanent blindness, but in zebrafish (Danio rerio), Müller glia function as intrinsic stem cells, producing progenitor cells that regenerate photoreceptors and restore vision. MicroRNAs (miRNAs) critically regulate neurogenesis in the brain and retina, but the roles of miRNAs in injury-induced neuronal regeneration are largely unknown. The miRNA miR-18a regulates photoreceptor differentiation in the embryonic retina. The purpose of the current study was to determine the function of miR-18a during injury-induced photoreceptor regeneration. RT-qPCR, in-situ hybridization (ISH) and immunohistochemistry (IHC) showed that miR-18a expression increases throughout the retina by 1-day post-injury (dpi) and continues to increase through 5 dpi. Bromodeoxyuridine (BrdU) labeling showed that at 7 and 10 dpi, when regenerated photoreceptors are normally differentiating, there are more proliferating Müller glia-derived progenitors in homozygous miR-18a mutant (miR-18ami5012) retinas compared with wild type (WT), indicating that miR-18a negatively regulates injury-induced proliferation. At 7 and 10 dpi, miR-18ami5012 retinas have fewer mature photoreceptors than WT, but there is no difference at 14 dpi, revealing that photoreceptor regeneration is delayed. BrdU labeling showed that the excess progenitors in miR-18ami5012 retinas migrate to other retinal layers besides the photoreceptor layer. Inflammation is critical for photoreceptor regeneration and RT-qPCR showed that, in the absence of miR-18a, inflammation is prolonged. Suppressing inflammation with dexamethasone rescues the miR-18ami5012 phenotype. Together, these data show that during injury-induced photoreceptor regeneration, miR-18a regulates proliferation and photoreceptor regeneration by regulating key aspects of the inflammatory response during photoreceptor regeneration in zebrafish.

scholarly journals Lamin B1 and lamin B2 are long-lived proteins with distinct functions in retinal development

2016 ◽  
Vol 27 (12) ◽  
pp. 1928-1937 ◽  
Author(s):  
David Razafsky ◽  
Candace Ward ◽  
Chloe Potter ◽  
Wanqiu Zhu ◽  
Yunlu Xue ◽  
...  
Keyword(s):  
Nuclear Lamina ◽  
Building Blocks ◽  
Postnatal Life ◽  
Cone Photoreceptors ◽  
Obvious Effect ◽  
Lamin B1 ◽  
Embryonic Neurogenesis ◽  
And Function ◽  
Embryonic Retina ◽  
Rod And Cone Photoreceptors

Lamin B1 and lamin B2 are essential building blocks of the nuclear lamina, a filamentous meshwork lining the nucleoplasmic side of the inner nuclear membrane. Deficiencies in lamin B1 and lamin B2 impair neurodevelopment, but distinct functions for the two proteins in the development and homeostasis of the CNS have been elusive. Here we show that embryonic depletion of lamin B1 in retinal progenitors and postmitotic neurons affects nuclear integrity, leads to the collapse of the laminB2 meshwork, impairs neuronal survival, and markedly reduces the cellularity of adult retinas. In stark contrast, a deficiency of lamin B2 in the embryonic retina has no obvious effect on lamin B1 localization or nuclear integrity in embryonic retinas, suggesting that lamin B1, but not lamin B2, is strictly required for nucleokinesis during embryonic neurogenesis. However, the absence of lamin B2 prevents proper lamination of adult retinal neurons, impairs synaptogenesis, and reduces cone photoreceptor survival. We also show that lamin B1 and lamin B2 are extremely long-lived proteins in rod and cone photoreceptors. OF interest, a complete absence of both proteins during postnatal life has little or no effect on the survival and function of cone photoreceptors.

Gene Expression Profiling of Zebrafish Embryonic Retina

Zebrafish ◽  
2005 ◽  
Vol 2 (4) ◽  
pp. 269-283 ◽  
Author(s):  
Yuk Fai Leung ◽  
John E. Dowling
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Embryonic Retina

Induction of glutamine synthetase in embryonic retina

1970 ◽  
Vol 61 (2-3) ◽  
pp. 342-346 ◽  
Author(s):  
T. Alescio ◽  
M. Moscona ◽  
A.A. Moscona
Keyword(s):  
Glutamine Synthetase ◽  
Embryonic Retina

Transplantation of human embryonic retina to adult rat retina

1990 ◽  
Vol 2 (1) ◽  
pp. 9-22 ◽  
Author(s):  
R. Aramant ◽  
M. Seiler ◽  
B. Ehinger ◽  
A. Bergström ◽  
B. Gustavii ◽  
...  
Keyword(s):  
Rat Retina ◽  
Adult Rat ◽  
Embryonic Retina

Age-Dependent Differences in Cognin Regeneration on Embryonic Retina Cells

1980 ◽  
Vol 3 (2) ◽  
pp. 66-74 ◽  
Author(s):  
Y.B. Ben-Shaul ◽  
R.E. Hausman ◽  
A.A. Moscona
Keyword(s):  
Age Dependent ◽  
Embryonic Retina
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