scholarly journals Coordinate control of gene expression noise and interchromosomal interactions in a MAP kinase pathway

2010 ◽  
Vol 12 (10) ◽  
pp. 954-962 ◽  
Author(s):  
Emma McCullagh ◽  
Anupama Seshan ◽  
Hana El-Samad ◽  
Hiten D. Madhani
Keyword(s):  
Gene Expression ◽  
Map Kinase ◽  
Control Of Gene Expression ◽  
Gene Expression Noise ◽  
Expression Noise ◽  
Coordinate Control ◽  
Map Kinase Pathway ◽  
Kinase Pathway

scholarly journals Translational control of gene expression noise and its relationship to ageing in yeast

FEBS Journal ◽  
2020 ◽  
Author(s):  
Tailise Carolina de Souza‐Guerreiro ◽  
Xiang Meng ◽  
Estelle Dacheux ◽  
Helena Firczuk ◽  
John McCarthy
Keyword(s):  
Gene Expression ◽  
Translational Control ◽  
Control Of Gene Expression ◽  
Gene Expression Noise ◽  
Expression Noise

Attenuation of Haptoglobin Gene Expression by TGFβ Requires the MAP Kinase Pathway

1999 ◽  
Vol 259 (3) ◽  
pp. 544-549 ◽  
Author(s):  
Shun-Jiang Yu ◽  
François Boudreau ◽  
Antoine Désilets ◽  
Mathieu Houde ◽  
Nathalie Rivard ◽  
...  
Keyword(s):  
Gene Expression ◽  
Map Kinase ◽  
Map Kinase Pathway ◽  
Haptoglobin Gene ◽  
Kinase Pathway

scholarly journals Orthogonal tuning of gene expression noise using CRISPR–Cas

2020 ◽  
Author(s):  
Fan Wu ◽  
Jiyoung Shim ◽  
Ting Gong ◽  
Cheemeng Tan
Keyword(s):  
Gene Expression ◽  
Reporter Gene ◽  
Transcription Initiation ◽  
Mrna Translation ◽  
Genetic Circuit ◽  
Control Of Gene Expression ◽  
Gene Expression Noise ◽  
Guide Rnas ◽  
Expression Noise ◽  
Genetic Components

Abstract The control of gene expression noise is important for improving drug treatment and the performance of synthetic biological systems. Previous work has tuned gene expression noise by changing the rate of transcription initiation, mRNA degradation, and mRNA translation. However, these methods are invasive: they require changes to the target genetic components. Here, we create an orthogonal system based on CRISPR-dCas9 to tune gene expression noise. Specifically, we modulate the gene expression noise of a reporter gene in Escherichia coli by incorporating CRISPR activation and repression (CRISPRar) simultaneously in a single cell. The CRISPRar uses a single dCas9 that recognizes two different single guide RNAs (sgRNA). We build a library of sgRNA variants with different expression activation and repression strengths. We find that expression noise and mean of a reporter gene can be tuned independently by CRISPRar. Our results suggest that the expression noise is tuned by the competition between two sgRNAs that modulate the binding of RNA polymerase to promoters. The CRISPRar may change how we tune expression noise at the genomic level. Our work has broad impacts on the study of gene functions, phenotypical heterogeneity, and genetic circuit control.

Lipopolysaccharide decreases SIGIRR/TIR8 gene expression possibly by suppressing SP1 via TLR4-p38 map kinase pathway

Cytokine ◽  
2009 ◽  
Vol 48 (1-2) ◽  
pp. 56-57
Author(s):  
Keiko Ueno-Shuto ◽  
Tsuyoshi Shuto ◽  
Kosuke Kato ◽  
Hiromichi Sakai ◽  
Tomomi Ono ◽  
...  
Keyword(s):  
Gene Expression ◽  
Map Kinase ◽  
P38 Map Kinase ◽  
Map Kinase Pathway ◽  
Kinase Pathway

scholarly journals Observation and Control of Gene Expression Noise: Barrier Crossing Analogies Between Drug Resistance and Metastasis

2020 ◽  
Vol 11 ◽  
Author(s):  
Michael Tyler Guinn ◽  
Yiming Wan ◽  
Sarah Levovitz ◽  
Dongbo Yang ◽  
Marsha R. Rosner ◽  
...  
Keyword(s):  
Gene Expression ◽  
Drug Resistance ◽  
Barrier Crossing ◽  
Control Of Gene Expression ◽  
Gene Expression Noise ◽  
Expression Noise ◽  
Noise Barrier ◽  
And Control
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