Fusion proteins comprising a Fusarium-specific antibody linked to antifungal peptides protect plants against a fungal pathogen

2004 ◽  
Vol 22 (6) ◽  
pp. 732-738 ◽  
Author(s):  
Dieter Peschen ◽  
He-Ping Li ◽  
Rainer Fischer ◽  
Fritz Kreuzaler ◽  
Yu-Cai Liao
Keyword(s):  
Specific Antibody ◽  
Fungal Pathogen ◽  
Fusion Proteins ◽  
Antifungal Peptides

scholarly journals Mannosylated Mucin-Type Immunoglobulin Fusion Proteins Enhance Antigen-Specific Antibody and T Lymphocyte Responses

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e46959 ◽  
Author(s):  
Gustaf Ahlén ◽  
Lena Strindelius ◽  
Tomas Johansson ◽  
Anki Nilsson ◽  
Nathalie Chatzissavidou ◽  
...  
Keyword(s):  
T Lymphocyte ◽  
Specific Antibody ◽  
Fusion Proteins ◽  
Lymphocyte Responses

scholarly journals TNFRSF receptor-specific antibody fusion proteins with targeting controlled FcγR-independent agonistic activity

2019 ◽  
Vol 10 (3) ◽  
Author(s):  
Juliane Medler ◽  
Johannes Nelke ◽  
Daniela Weisenberger ◽  
Tim Steinfatt ◽  
Moritz Rothaug ◽  
...  
Keyword(s):  
Specific Antibody ◽  
Fusion Proteins ◽  
Agonistic Activity

scholarly journals CD40- and CD95-specific antibody single chain-Baff fusion proteins display BaffR-, TACI- and BCMA-restricted agonism

mAbs ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 1807721
Author(s):  
Johannes Nelke ◽  
Juliane Medler ◽  
Daniela Weisenberger ◽  
Andreas Beilhack ◽  
Harald Wajant
Keyword(s):  
Specific Antibody ◽  
Fusion Proteins ◽  
Single Chain

scholarly journals Antifungal potency and modes of action of a novel olive tree defensin against closely related ascomycete fungal pathogens

2019 ◽  
Author(s):  
Hui Li ◽  
Siva L. S. Velivelli ◽  
Dilip M. Shah
Keyword(s):  
Antifungal Activity ◽  
Fungal Pathogen ◽  
Fungal Pathogens ◽  
Olive Tree ◽  
In Planta ◽  
Core Motif ◽  
Antifungal Peptides ◽  
Cultivated Species ◽  
High Concentrations

AbstractAntimicrobial peptides play a pivotal role in the innate immunity of plants. Defensins are cysteine-rich antifungal peptides with multiple mechanisms of action (MOA). A novel Oleaceae-specific defensin gene family was discovered in the genome sequences of the wild and cultivated species of a perennial olive tree, Olea europaea. Antifungal properties of an olive tree defensin OefDef1.1 were investigated against a necrotrophic ascomycete fungal pathogen Botrytis cinerea in vitro and in planta. OefDef1.1 displayed potent antifungal activity against this pathogen by rapidly permeabilizing the plasma membrane of the conidial and germling cells. Interestingly, it was translocated to the cytoplasm and induced reactive oxygen species in the germlings, but not in the conidia. In medium containing high concentrations of Na1+, antifungal activity of OefDef1.1 against B. cinerea was significantly reduced. In contrast, OefDef1.1_V1 variant in which the γ-core motif of OefDef1.1 was replaced by that of a Medicago truncatula defensin MtDef4 displayed Na1+-tolerant antifungal activity and was more potent in reducing the virulence of B. cinerea in planta. OefDef1.1 also exhibited potent antifungal activity against three hemibiotrophic ascomycete pathogens Fusarium graminearum, F. oxysporum and F. virguliforme. Significant differences were observed among the four pathogens in their responses to OefDef1.1 in growth medium with or without the high concentrations of Na1+. The varied responses of closely related ascomycete pathogens to this defensin have implications for engineering disease resistance in plants.

scholarly journals CD40- and 41BB-specific antibody fusion proteins with PDL1 blockade-restricted agonism

Theranostics ◽  
2022 ◽  
Vol 12 (4) ◽  
pp. 1486-1499
Author(s):  
Juliane Medler ◽  
Kirstin Kucka ◽  
Vinicio Melo ◽  
Tengyu Zhang ◽  
Stefan von Rotenhan ◽  
...  
Keyword(s):  
Specific Antibody ◽  
Fusion Proteins

scholarly journals Released ATP Is an Extracellular Cytotoxic Mediator in Salivary Histatin 5-Induced Killing of Candida albicans

2000 ◽  
Vol 68 (12) ◽  
pp. 6848-6856 ◽  
Author(s):  
Svetlana E. Koshlukova ◽  
Marcelo W. B. Araujo ◽  
Didi Baev ◽  
Mira Edgerton
Keyword(s):  
Cell Death ◽  
Candida Albicans ◽  
Fungal Pathogen ◽  
Atp Release ◽  
Therapeutic Agents ◽  
Extracellular Atp ◽  
Histatin 5 ◽  
Antifungal Peptides ◽  
Atp Analogues ◽  
Atp Efflux

ABSTRACT Salivary histatins (Hsts) are antifungal peptides with promise as therapeutic agents against candidiasis. Hst 5 kills the fungal pathogenCandida albicans via a mechanism that involves release of cellular ATP in the absence of cytolysis. Here we demonstrate that released ATP has a further role in Hst 5 killing. Incubation of the cells with ATP analogues induced cell death, and addition of the ATP scavenger apyrase to remove extracellular ATP released during Hst 5 treatment resulted in a reduction in cell killing. Experiments using anaerobically grown C. albicans with decreased susceptibility to Hst 5 confirmed that depletion of cellular ATP as a result of ATP efflux was not sufficient to cause cell death. In contrast to Hst-susceptible aerobic cultures, anaerobically grown cells were not killed by exogenously applied ATP. These findings established that Hst binding, subsequent entry into the cells, and ATP release precede the signal for cytotoxicity, which is mediated by extracellular ATP. In a higher-eukaryote paradigm, released ATP acts as a cytotoxic mediator by binding to membrane nucleotide P2X receptors. Based on a pharmacological profile and detection of a C. albicans60-kDa membrane protein immunoreactive with antibody to P2X7 receptor, we propose that released ATP in response to Hst 5 activates candidal P2X7-like receptors to cause cell death.

Studies on the Fungal Pathogen of Dutch Elm Disease

1981 ◽  
Vol 39 ◽  
pp. 400-401
Author(s):  
H.M. Mazzone ◽  
G. Wray ◽  
R. Zerillo
Keyword(s):  
Fungal Pathogen ◽  
Fungal Growth ◽  
Polymyxin B ◽  
The United States ◽  
Dutch Elm Disease ◽  
Effective Control ◽  
Sabouraud Agar ◽  
Total Inhibition ◽  
Zones Of Inhibition ◽  
Control Plate

The fungal pathogen of the Dutch elm disease (DED), Ceratocystis ulmi (Buisman) C. Moreau, has eluded effective control since its introduction in the United States more than sixty years ago. Our studies on DED include establishing biological control agents against C. ulmi. In this report we describe the inhibitory action of the antibiotic polymyxin B on the causal agent of DED.In screening a number of antibiotics against C. ulmi, we observed that filter paper discs containing 300 units (U) of polymyxin B (Difco Laboratories) per disc, produced zones of inhibition to the fungus grown on potato dextrose agar or Sabouraud agar plates (100mm x 15mm), Fig. 1a. Total inhibition of fungal growth on a plate occurred when agar overlays containing fungus and antibiotic (polymyxin B sulfate, ICN Pharmaceuticals, Inc.) were poured on the underlying agar growth medium. The agar overlays consisted of the following: 4.5 ml of 0.7% agar, 0.5 ml of fungus (control plate); 4.0 ml of 0.7% agar, 0.5 ml of fungus, 0.5 ml of polymyxin B sulfate (77,700 U). Fig. 1, b and c, compares a control plate and polymyxin plate after seven days.

A Sensitive On-Grid Immunoelectron Microscopic Procedure for Diagnosis of Rotavirus Infection

1980 ◽  
Vol 38 ◽  
pp. 506-507
Author(s):  
M. F. Miller ◽  
A. R. Rubenstein
Keyword(s):  
Electron Microscopy ◽  
Specific Antibody ◽  
Immune Complexes ◽  
Acute Gastroenteritis ◽  
Plant Viruses ◽  
Aggregation Process ◽  
Microscopic Technique ◽  
Fecal Material ◽  
Present Communication ◽  
Number Of Particles

Studies of rotavirus particles in humans, monkeys and various non-primates with acute gastroenteritis have involved detection of virus in fecal material by electron microscopy. The EM techniques most commonly employed have been the conventional negative staining (Fig. 1) and immune aggregation (Fig. 2) procedures. Both methods are somewhat insensitive and can most reliably be applied to samples containing large quantities of virus either naturaLly or as a result of concentration by ultracentrifugation. The formation of immune complexes by specific antibody in the immune aggregation procedures confirms the rotavirus diagnosis, but the number of particles per given microscope field is effectively reduced by the aggregation process. In the present communication, we describe use of an on-grid immunoelectron microscopic technique in which rotavirus particles are mounted onto microscope grids that were pre-coated with specific antibody. The technique is a modification of a method originalLy introduced by Derrick (1) for studies of plant viruses.

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