An improved cyan fluorescent protein variant useful for FRET

2004 ◽  
Vol 22 (4) ◽  
pp. 445-449 ◽  
Author(s):  
Mark A Rizzo ◽  
Gerald H Springer ◽  
Butch Granada ◽  
David W Piston
Keyword(s):  
Fluorescent Protein ◽  
Cyan Fluorescent Protein ◽  
Protein Variant

scholarly journals A Photostable Green Fluorescent Protein Variant for Analysis of Protein Localization in Candida albicans

2009 ◽  
Vol 9 (1) ◽  
pp. 224-226 ◽  
Author(s):  
Chengda Zhang ◽  
James B. Konopka
Keyword(s):  
Candida Albicans ◽  
Green Fluorescent Protein ◽  
Codon Usage ◽  
Signal Intensity ◽  
Fluorescent Protein ◽  
Protein Localization ◽  
Protein Variant ◽  
Green Fluorescent ◽  
Green Fluorescent Protein Variant

ABSTRACT Fusions to the green fluorescent protein (GFP) are an effective way to monitor protein localization. However, altered codon usage in Candida species has delayed implementation of new variants. Examination of three new GFP variants in Candida albicans showed that one has higher signal intensity and increased resistance to photobleaching.

Ultrafast Proton Shuttling in Psammocora Cyan Fluorescent Protein

2013 ◽  
Vol 117 (38) ◽  
pp. 11134-11143 ◽  
Author(s):  
John T. M. Kennis ◽  
Ivo H. M. van Stokkum ◽  
Dayna S. Peterson ◽  
Anjali Pandit ◽  
Rebekka M. Wachter
Keyword(s):  
Fluorescent Protein ◽  
Cyan Fluorescent Protein ◽  
Proton Shuttling

scholarly journals Specific expression of an oxytocin-enhanced cyan fluorescent protein fusion transgene in the rat hypothalamus and posterior pituitary

2009 ◽  
Vol 204 (3) ◽  
pp. 275-285 ◽  
Author(s):  
Akiko Katoh ◽  
Hiroaki Fujihara ◽  
Toyoaki Ohbuchi ◽  
Tatsushi Onaka ◽  
W Scott Young ◽  
...  
Keyword(s):  
Fluorescent Protein ◽  
Fusion Gene ◽  
Plasma Osmolality ◽  
Cyan Fluorescent Protein ◽  
Posterior Pituitary ◽  
Protein Fusion ◽  
Transgenic Rats ◽  
Salt Loading ◽  
Wide Range ◽  
Enhanced Cyan Fluorescent Protein

We have generated rats bearing an oxytocin (OXT)-enhanced cyan fluorescent protein (eCFP) fusion transgene designed from a murine construct previously shown to be faithfully expressed in transgenic mice. In situ hybridisation histochemistry revealed that the Oxt–eCfp fusion gene was expressed in the supraoptic nucleus (SON) and the paraventricular nucleus (PVN) in these rats. The fluorescence emanating from eCFP was observed only in the SON, the PVN, the internal layer of the median eminence and the posterior pituitary (PP). In in vitro preparations, freshly dissociated cells from the SON and axon terminals showed clear eCFP fluorescence. Immunohistochemistry for OXT and arginine vasopressin (AVP) revealed that the eCFP fluorescence co-localises with OXT immunofluorescence, but not with AVP immunofluorescence in the SON and the PVN. Although the expression levels of the Oxt–eCfp fusion gene in the SON and the PVN showed a wide range of variations in transgenic rats, eCFP fluorescence was markedly increased in the SON and the PVN, but decreased in the PP after chronic salt loading. The expression of the Oxt gene was significantly increased in the SON and the PVN after chronic salt loading in both non-transgenic and transgenic rats. Compared with wild-type animals, euhydrated and salt-loaded male and female transgenic rats showed no significant differences in plasma osmolality, sodium concentration and OXT and AVP levels, suggesting that the fusion gene expression did not disturb any physiological processes. These results suggest that our new transgenic rats are a valuable new tool to identify OXT-producing neurones and their terminals.

Cyan Fluorescent Protein Carries a Constitutive Mutation That Prevents Its Dimerization

Biochemistry ◽  
2011 ◽  
Vol 50 (4) ◽  
pp. 437-439 ◽  
Author(s):  
Agathe Espagne ◽  
Marie Erard ◽  
Karine Madiona ◽  
Valérie Derrien ◽  
Gabriella Jonasson ◽  
...  
Keyword(s):  
Fluorescent Protein ◽  
Cyan Fluorescent Protein ◽  
Constitutive Mutation

Monitoring Protein Interactions in the Living Cell Through the Fluorescence Decays of the Cyan Fluorescent Protein

ChemPhysChem ◽  
2006 ◽  
Vol 7 (7) ◽  
pp. 1442-1454 ◽  
Author(s):  
Regis Grailhe ◽  
Fabienne Merola ◽  
Jacqueline Ridard ◽  
Stephen Couvignou ◽  
Chantal Le Poupon ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Living Cell ◽  
Fluorescent Protein ◽  
Cyan Fluorescent Protein
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