Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots

2002 ◽  
Vol 21 (1) ◽  
pp. 41-46 ◽  
Author(s):  
Xingyong Wu ◽  
Hongjian Liu ◽  
Jianquan Liu ◽  
Kari N. Haley ◽  
Joseph A. Treadway ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Semiconductor Quantum Dots ◽  
Cancer Marker ◽  
Cellular Targets

scholarly journals Erratum: Corrigendum: Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots

2003 ◽  
Vol 21 (4) ◽  
pp. 452-452 ◽  
Author(s):  
Xingyong Wu ◽  
Hongjian Liu ◽  
Jianquan Liu ◽  
Kari N. Haley ◽  
Joseph A. Treadway ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Semiconductor Quantum Dots ◽  
Cancer Marker ◽  
Cellular Targets

scholarly journals Multispectral imaging of clinically relevant cellular targets in tonsil and lymphoid tissue using semiconductor quantum dots

2006 ◽  
Vol 19 (9) ◽  
pp. 1181-1191 ◽  
Author(s):  
Thomas J Fountaine ◽  
Stephen M Wincovitch ◽  
David H Geho ◽  
Susan H Garfield ◽  
Stefania Pittaluga
Keyword(s):  
Quantum Dots ◽  
Lymphoid Tissue ◽  
Multispectral Imaging ◽  
Semiconductor Quantum Dots ◽  
Cellular Targets

scholarly journals Evaluation of quantum dot conjugated antibodies for immunofluorescent labelling of cellular targets

2017 ◽  
Vol 8 ◽  
pp. 1238-1249 ◽  
Author(s):  
Jennifer E Francis ◽  
David Mason ◽  
Raphaël Lévy
Keyword(s):  
Quantum Dots ◽  
Fluorescent Dyes ◽  
Fluorescent Proteins ◽  
Semiconductor Quantum Dots ◽  
Secondary Antibody ◽  
Igg Antibodies ◽  
Protein Targets ◽  
Cellular Targets ◽  
Extensive Cell ◽  
Specific Labelling

Semiconductor quantum dots (Qdots) have been utilised as probes in fluorescence microscopy and provide an alternative to fluorescent dyes and fluorescent proteins due to their brightness, photostability, and the possibility to excite different Qdots with a single wavelength. In spite of these attractive properties, their implemenation by biologists has been somewhat limited and only a few Qdot conjugates are commercially available for the labelling of cellular targets. Although many protocols have been reported for the specific labelling of proteins with Qdots, the majority of these relied on Qdot-conjugated antibodies synthesised specifically by the authors (and therefore not widely available), which limits the scope of applications and complicates replication. Here, the specificity of a commercially available, Qdot-conjugated secondary antibody (Qdot-Ab) was tested against several primary IgG antibodies. The antigens were labelled simultaneously with a fluorescent dye coupled to a secondary antibody (Dye-Ab) and the Qdot-Ab. Although, the Dye-Ab labelled all of the intended target proteins, the Qdot-Ab was found bound to only some of the protein targets in the cytosol and could not reach the nucleus, even after extensive cell permeabilisation.

scholarly journals Evaluation of quantum dot conjugated antibodies for immunofluorescent labelling of cellular targets

2016 ◽  
Author(s):  
Jennifer E. Francis ◽  
David Mason ◽  
Raphaël Lévy
Keyword(s):  
Quantum Dots ◽  
Fluorescent Dyes ◽  
Fluorescent Proteins ◽  
Fluorescent Microscopy ◽  
Semiconductor Quantum Dots ◽  
Secondary Antibody ◽  
Protein Targets ◽  
Cellular Targets ◽  
Extensive Cell ◽  
Specific Labelling

AbstractSemiconductor quantum dots (Qdots) have been utilised as probes in fluorescent microscopy and provide an alternative to fluorescent dyes and fluorescent proteins, due to their brightness, photostability, and the possibility to excite different Qdots with a single wavelength. In spite of these attractive properties, their take up by biologists has been somewhat limited and only a few Qdot conjugates are commercially available for the labelling of cellular targets. Although, many protocols have been reported for the specific labelling of proteins with Qdots, the majority of these relied on Qdot-conjugated antibodies synthesised specifically by the authors and therefore not broadly available, which limits the scope of applications and complicates replication. Here, the specificity of a commercially available Qdot conjugated secondary antibody (Qdot-Ab), for different antigens, was tested. Antigens were labelled simultaneously with a fluorescent dye coupled to a secondary antibody (Dye-Ab) and the Qdot-Ab. Although, the Dye-Ab labelled all of the intended target proteins, the Qdot-Ab only bound to some of the protein targets in the cytosol and could not reach the nucleus even after extensive cell permeabilisation.

Semiconductor Quantum Dots for Multicolor Fluorescence Imaging and Spectroscopy of Single Cancer Cells

2003 ◽  
Vol 773 ◽  
Author(s):  
Xiaohu Gao ◽  
Shuming Nie ◽  
Wallace H. Coulter
Keyword(s):  
Quantum Dots ◽  
Cancer Cells ◽  
Fluorescence Imaging ◽  
Organic Dyes ◽  
Fluorescent Proteins ◽  
Single Cells ◽  
Quantitative Imaging ◽  
Semiconductor Quantum Dots ◽  
Single Cancer ◽  
Imaging And Spectroscopy

AbstractLuminescent quantum dots (QDs) are emerging as a new class of biological labels with unique properties and applications that are not available from traditional organic dyes and fluorescent proteins. Here we report new developments in using semiconductor quantum dots for quantitative imaging and spectroscopy of single cancer cells. We show that both live and fixed cells can be labeled with multicolor QDs, and that single cells can be analyzed by fluorescence imaging and wavelength-resolved spectroscopy. These results raise new possibilities in cancer imaging, molecular profiling, and disease staging.

Hot Topic and Challenge of Semiconductor Quantum Dots as Fluorescence Labels*

2010 ◽  
Vol 37 (1) ◽  
pp. 103-110 ◽  
Author(s):  
Chang-Yan LI ◽  
Qian LI ◽  
Hai-Tao LIU ◽  
Jun ZHANG ◽  
DAMIRIN Aletangaole
Keyword(s):  
Quantum Dots ◽  
Semiconductor Quantum Dots
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