scholarly journals Combined small-molecule inhibition accelerates developmental timing and converts human pluripotent stem cells into nociceptors

2012 ◽  
Vol 30 (7) ◽  
pp. 715-720 ◽  
Author(s):  
Stuart M Chambers ◽  
Yuchen Qi ◽  
Yvonne Mica ◽  
Gabsang Lee ◽  
Xin-Jun Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Small Molecule ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Developmental Timing ◽  
Small Molecule Inhibition

scholarly journals Combined small-molecule inhibition accelerates the derivation of functional cortical neurons from human pluripotent stem cells

2017 ◽  
Vol 35 (2) ◽  
pp. 154-163 ◽  
Author(s):  
Yuchen Qi ◽  
Xin-Jun Zhang ◽  
Nicolas Renier ◽  
Zhuhao Wu ◽  
Talia Atkin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Small Molecule ◽  
Pluripotent Stem Cells ◽  
Cortical Neurons ◽  
Human Pluripotent Stem Cells ◽  
Small Molecule Inhibition

scholarly journals Aryl hydrocarbon receptor inhibition promotes hematolymphoid development from human pluripotent stem cells

Blood ◽  
2017 ◽  
Vol 129 (26) ◽  
pp. 3428-3439 ◽  
Author(s):  
Mathew G. Angelos ◽  
Paige N. Ruh ◽  
Beau R. Webber ◽  
Robert H. Blum ◽  
Caitlin D. Ryan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Differentiation ◽  
Aryl Hydrocarbon Receptor ◽  
Small Molecule ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Aryl Hydrocarbon ◽  
Small Molecule Inhibition ◽  
Receptor Inhibition ◽  
Key Points

Key Points Small-molecule inhibition and CRISPR/Cas9 deletion of AHR promote early hematoendothelial cell differentiation from hESCs. AHR inhibition enhances the differentiation of cNK cells from hESCs whereas AHR hyperactivation supports development of ILC3s.

scholarly journals Small-Molecule-Driven Hepatocyte Differentiation of Human Pluripotent Stem Cells

2015 ◽  
Vol 4 (5) ◽  
pp. 939-952 ◽  
Author(s):  
Richard Siller ◽  
Sebastian Greenhough ◽  
Elena Naumovska ◽  
Gareth J. Sullivan
Keyword(s):  
Stem Cells ◽  
Small Molecule ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Hepatocyte Differentiation

scholarly journals Efficiently generate functional hepatic cells from human pluripotent stem cells by complete small-molecule strategy

2021 ◽  
Author(s):  
Tingcai Pan ◽  
Ning Wang ◽  
Jiaye Zhang ◽  
Fan Yang ◽  
Yan Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Small Molecules ◽  
Small Molecule ◽  
Pluripotent Stem Cells ◽  
Cost Effective ◽  
Human Pluripotent Stem Cells ◽  
Scale Production ◽  
Hepatic Cells ◽  
Cell Based Therapy

Abstract Background: Various methods have been developed to generate hepatic cells from human pluripotent stem cells (hPSCs) that rely on the combined use of multiple expensive growth factors, limiting industrial-scale production and widespread applications. Small molecules offer an attractive alternative to growth factors for producing hepatic cells since they are more economical and relatively stable. Methods: We dissect small-molecule combinations and identify the ideal cocktails to achieve an optimally efficient and cost-effective strategy for hepatic cells differentiation, expansion, and maturation.Results: We demonstrated that small-molecule cocktail CIP efficiently induced definitive endoderm (DE) formation via increased endogenous TGF-β/Nodal signaling. Furthermore, we identified that combining Vitamin C, Dihexa, and Forskolin (VDF) could substitute growth factors to induce hepatic specification. The obtained hepatoblasts (HBs) could subsequently expand and mature into functional hepatocyte-like cells (HLCs) by the established chemical formulas. Thus, we established a stepwise strategy with complete small molecules for efficiently producing scalable HBs and functionally matured HLCs. The small-molecule derived HLCs displayed typical functional characteristics as mature hepatocytes in vitro and repopulating injured liver in vivo. Conclusion: Our current small-molecule based hepatic generation protocol presents an efficient and cost-effective platform for the large-scale production of functional human hepatic cells for cell-based therapy and drug discovery using.

scholarly journals Small Molecule-Assisted, Line-Independent Maintenance of Human Pluripotent Stem Cells in Defined Conditions

PLoS ONE ◽  
2012 ◽  
Vol 7 (7) ◽  
pp. e41958 ◽  
Author(s):  
Stefan Frank ◽  
Miao Zhang ◽  
Hans R. Schöler ◽  
Boris Greber
Keyword(s):  
Stem Cells ◽  
Small Molecule ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells
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