scholarly journals Genome sequence of foxtail millet (Setaria italica) provides insights into grass evolution and biofuel potential

2012 ◽  
Vol 30 (6) ◽  
pp. 549-554 ◽  
Author(s):  
Gengyun Zhang ◽  
Xin Liu ◽  
Zhiwu Quan ◽  
Shifeng Cheng ◽  
Xun Xu ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Foxtail Millet ◽  
Setaria Italica ◽  
Grass Evolution

scholarly journals De Novo Genome Assembly of a Foxtail Millet Cultivar Huagu11 Uncovered the Genetic Difference to the Cultivar Yugu1, and the Genetic Mechanism of Imazethapyr Tolerance

2021 ◽  
Author(s):  
Jie Wang ◽  
Shiming Li ◽  
Lei Lan ◽  
Mushan Xie ◽  
Shu Cheng ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Genome Sequence ◽  
Reference Genome ◽  
Foxtail Millet ◽  
Setaria Italica ◽  
Genome Comparison ◽  
Sequencing Technology ◽  
High Quality ◽  
Next Generation Sequencing Technology ◽  
Generation Sequencing

Abstract Background: Setaria italica is the second-most widely planted species of millets in the world and an important model grain crop for the research of C4 photosynthesis and abiotic stress tolerance. Through three genomes assembly and annotation efforts, all genomes were based on next generation sequencing technology, which limited the genome continuity. Results: Here we report a high-quality whole-genome of new cultivar Huagu11, using single-molecule real-time sequencing and High-throughput chromosome conformation capture (Hi-C) mapping technologies. The total assembly size of the Huagu11 genome was 408.37 Mb with a scaffold N50 size of 45.89 Mb. Compared with the other three reported millet genomes based on the next generation sequencing technology, the Huagu11 genome had the highest genomic continuity. Intraspecies comparison showed about 94.97% and 94.66% of the Yugu1 and Huagu11 genomes, respectively, were able to be aligned as one-to-one blocks with four chromosome inversion. The Huagu11 genome contained approximately 19.43 Mb Presence/absence Variation (PAV) with 627 protein-coding transcripts, while Yugu1 genomes had 20.53 Mb PAV sequences encoding 737 proteins. Overall, 969,596 Single-nucleotide polymorphism (SNPs) and 156,282 insertion-deletion (InDels) were identified between these two genomes. The genome comparison between Huagu11 and Yugu1 should reflect the genetic identity and variation between the cultivars of foxtail millet to a certain extent. The Ser-626-Aln substitution in acetohydroxy acid synthase (AHAS) was found to be relative to the imazethapyr tolerance in Huagu11. Conclusions: A new improved high-quality reference genome sequence of Setaria italica was assembled, and intraspecies genome comparison determined the genetic identity and variation between the cultivars of foxtail millet. Based on the genome sequence, it was found that the Ser-626-Aln substitution in AHAS was responsible for the imazethapyr tolerance in Huagu11. The new improved reference genome of Setaria italica will promote the genic and genomic studies of this species and be beneficial for cultivar improvement.

Target Gene Identification and sgRNA Design for Waterlogging Tolerance in Foxtail Millet via CRISPR-based Transcriptional Activation

2021 ◽  
Vol 01 ◽  
Author(s):  
Siti Nor Akmar Abdullah ◽  
Sean Mayes ◽  
Mahdi Moradpour
Keyword(s):  
Genome Sequence ◽  
Transcriptional Activation ◽  
Target Gene ◽  
Gene Activation ◽  
Foxtail Millet ◽  
Setaria Italica ◽  
Waterlogging Tolerance ◽  
Activation Domains ◽  
Functional Studies ◽  
Cereal Species

Background: CRISPR activation (CRISPRa) uses non-functional Cas9 endonuclease (dCas9) but retains the genome targeting ability through its single guide RNAs (sgRNAs). CRISPRa is widely utilized as a gene activation system exploiting its ability to recruit various transcriptional activation domains (TADs) to enhance the expression of the target gene(s). Drought tolerant and resource-efficient crops like millets can mitigate the effects of climate change and strengthen food security. Objective: This study aimed to use the Setaria italica (foxtail millet) genome sequence to identify a target gene and the subsequent generation of sgRNAs for use in CRISPRa for conferring waterlogging tolerance that will benefit the future expansion of its cultivation area. Methods and Results: Leveraging existing RNA-seq data and information on functional studies in model plants and from other cereal species, maize and barley, have enabled the identification of candidate ERFVII from the foxtail millet genome sequence in the attempt to engineer waterlogging tolerance. The study provides a step-by-step example for using publicly accessible databases and bioinformatics tools from NCBI and Phytozome to identify and characterize the ortholog from Setaria italica. Softberry was used for promoter annotation to obtain the transcription start site (TSS). Subsequently, CRISP-P 2.0 design tools were employed to generate and select a few efficient sgRNAs for CRISPRa that minimize potentially deleterious off-target binding. Conclusion: The study is a helpful example of how to advance in genomics research, including the revolutionizing CRISPR technology in Setaria italica, which can be adopted in other plant species by utilizing the available genome sequence.

scholarly journals De novo genome assembly of a foxtail millet cultivar Huagu11 uncovered the genetic difference to the cultivar Yugu1, and the genetic mechanism of imazethapyr tolerance

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Jie Wang ◽  
Shiming Li ◽  
Lei Lan ◽  
Mushan Xie ◽  
Shu Cheng ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Genome Sequence ◽  
Reference Genome ◽  
Foxtail Millet ◽  
Setaria Italica ◽  
Genome Comparison ◽  
Sequencing Technology ◽  
High Quality ◽  
Next Generation Sequencing Technology ◽  
Generation Sequencing

Abstract Background Setaria italica is the second-most widely planted species of millets in the world and an important model grain crop for the research of C4 photosynthesis and abiotic stress tolerance. Through three genomes assembly and annotation efforts, all genomes were based on next generation sequencing technology, which limited the genome continuity. Results Here we report a high-quality whole-genome of new cultivar Huagu11, using single-molecule real-time sequencing and High-throughput chromosome conformation capture (Hi-C) mapping technologies. The total assembly size of the Huagu11 genome was 408.37 Mb with a scaffold N50 size of 45.89 Mb. Compared with the other three reported millet genomes based on the next generation sequencing technology, the Huagu11 genome had the highest genomic continuity. Intraspecies comparison showed about 94.97 and 94.66% of the Yugu1 and Huagu11 genomes, respectively, were able to be aligned as one-to-one blocks with four chromosome inversion. The Huagu11 genome contained approximately 19.43 Mb Presence/absence Variation (PAV) with 627 protein-coding transcripts, while Yugu1 genomes had 20.53 Mb PAV sequences encoding 737 proteins. Overall, 969,596 Single-nucleotide polymorphism (SNPs) and 156,282 insertion-deletion (InDels) were identified between these two genomes. The genome comparison between Huagu11 and Yugu1 should reflect the genetic identity and variation between the cultivars of foxtail millet to a certain extent. The Ser-626-Aln substitution in acetohydroxy acid synthase (AHAS) was found to be relative to the imazethapyr tolerance in Huagu11. Conclusions A new improved high-quality reference genome sequence of Setaria italica was assembled, and intraspecies genome comparison determined the genetic identity and variation between the cultivars of foxtail millet. Based on the genome sequence, it was inferred that the Ser-626-Aln substitution in AHAS was responsible for the imazethapyr tolerance in Huagu11. The new improved reference genome of Setaria italica will promote the genic and genomic studies of this species and be beneficial for cultivar improvement.

Variation and Correlation Analysis of Hay Forage Quality Traits of Foxtail Millet [Setaria italica (L.) Beauv.]

2013 ◽  
Vol 38 (5) ◽  
pp. 800-807
Author(s):  
Hui ZHI ◽  
Zhen-Gang NIU ◽  
Guan-Qing JIA ◽  
Yang CHAI ◽  
Wei LI ◽  
...  
Keyword(s):  
Correlation Analysis ◽  
Foxtail Millet ◽  
Forage Quality ◽  
Setaria Italica ◽  
Quality Traits

Genetic Studies with Foxtail Millet, Setaria Italica (L.) Beauv. 1

1940 ◽  
Vol 32 (6) ◽  
pp. 426-438 ◽  
Author(s):  
H. W. Li ◽  
J. C. Meng ◽  
C. H. Li
Keyword(s):  
Foxtail Millet ◽  
Setaria Italica ◽  
Genetic Studies

scholarly journals Establishing in planta haploid inducer line by edited SiMTL in foxtail millet ( Setaria italica )

2021 ◽  
Author(s):  
Zixiang Cheng ◽  
Yao Sun ◽  
Suhua Yang ◽  
Hui Zhi ◽  
Tao Yin ◽  
...  
Keyword(s):  
Foxtail Millet ◽  
Setaria Italica ◽  
In Planta ◽  
Haploid Inducer

scholarly journals Plant-based milk Developed from Soy (Glycine max) Milk and Foxtail Millet (Setaria italica)

2021 ◽  
Vol 807 (2) ◽  
pp. 022063
Author(s):  
Dzul Fadly ◽  
Wiwi Ulfairah Sutarno ◽  
Yuges Saputri Muttalib ◽  
Masturi Muhajir ◽  
Fatimah Fitriani Mujahidah
Keyword(s):  
Glycine Max ◽  
Foxtail Millet ◽  
Setaria Italica

scholarly journals Phytolith Analysis for Differentiating between Foxtail Millet (Setaria italica) and Green Foxtail (Setaria viridis)

PLoS ONE ◽  
2011 ◽  
Vol 6 (5) ◽  
pp. e19726 ◽  
Author(s):  
Jianping Zhang ◽  
Houyuan Lu ◽  
Naiqin Wu ◽  
Xiaoyan Yang ◽  
Xianmin Diao
Keyword(s):  
Foxtail Millet ◽  
Setaria Italica ◽  
Setaria Viridis ◽  
Phytolith Analysis ◽  
Green Foxtail
Sign in / Sign up

Export Citation Format

Share Document