scholarly journals Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips

2010 ◽  
Vol 28 (12) ◽  
pp. 1295-1299 ◽  
Author(s):  
Sriram Kosuri ◽  
Nikolai Eroshenko ◽  
Emily M LeProust ◽  
Michael Super ◽  
Jeffrey Way ◽  
...  
Keyword(s):  
Gene Synthesis ◽  
High Fidelity ◽  
Selective Amplification ◽  
Dna Pools

scholarly journals DropSynth 2.0: high-fidelity multiplexed gene synthesis in emulsions

2020 ◽  
Vol 48 (16) ◽  
pp. e95-e95 ◽  
Author(s):  
Angus M Sidore ◽  
Calin Plesa ◽  
Joyce A Samson ◽  
Nathan B Lubock ◽  
Sriram Kosuri
Keyword(s):  
Error Correction ◽  
Low Cost ◽  
Gene Synthesis ◽  
High Fidelity ◽  
Functional Testing ◽  
Gene Length ◽  
Genetic Elements ◽  
Gene Libraries

Abstract Multiplexed assays allow functional testing of large synthetic libraries of genetic elements, but are limited by the designability, length, fidelity and scale of the input DNA. Here, we improve DropSynth, a low-cost, multiplexed method that builds gene libraries by compartmentalizing and assembling microarray-derived oligonucleotides in vortexed emulsions. By optimizing enzyme choice, adding enzymatic error correction and increasing scale, we show that DropSynth can build thousands of gene-length fragments at >20% fidelity.

scholarly journals DropSynth 2.0: high-fidelity multiplexed gene synthesis in emulsions

2019 ◽  
Author(s):  
Angus M. Sidore ◽  
Calin Plesa ◽  
Joyce A. Samson ◽  
Sriram Kosuri
Keyword(s):  
Error Correction ◽  
Low Cost ◽  
Gene Synthesis ◽  
High Fidelity ◽  
Functional Testing ◽  
Gene Length ◽  
Genetic Elements ◽  
Gene Libraries

AbstractMultiplexed assays allow functional testing of large synthetic libraries of genetic elements, but are limited by the designability, length, fidelity and scale of the input DNA. Here we improve DropSynth, a low-cost, multiplexed method which builds gene libraries by compartmentalizing and assembling microarray-derived oligos in vortexed emulsions. By optimizing enzyme choice, adding enzymatic error correction, and increasing scale, we show that DropSynth can build thousands of gene-length fragments at >20% fidelity.

scholarly journals High-fidelity gene synthesis by retrieval of sequence-verified DNA identified using high-throughput pyrosequencing

2010 ◽  
Vol 28 (12) ◽  
pp. 1291-1294 ◽  
Author(s):  
Mark Matzas ◽  
Peer F Stähler ◽  
Nathalie Kefer ◽  
Nicole Siebelt ◽  
Valesca Boisguérin ◽  
...  
Keyword(s):  
High Throughput ◽  
Gene Synthesis ◽  
High Fidelity

Using the Theory of Planned Behavior to Predict Faking in Selection Exercises Varying in Fidelity

2018 ◽  
Vol 17 (3) ◽  
pp. 155-160 ◽  
Author(s):  
Daniel Dürr ◽  
Ute-Christine Klehe
Keyword(s):  
Theory Of Planned Behavior ◽  
Planned Behavior ◽  
Predictive Validity ◽  
Group Discussion ◽  
Role Play ◽  
Selection Procedure ◽  
High Fidelity ◽  
Selection Research ◽  
Play Group

Abstract. Faking has been a concern in selection research for many years. Many studies have examined faking in questionnaires while far less is known about faking in selection exercises with higher fidelity. This study applies the theory of planned behavior (TPB; Ajzen, 1991 ) to low- (interviews) and high-fidelity (role play, group discussion) exercises, testing whether the TPB predicts reported faking behavior. Data from a mock selection procedure suggests that candidates do report to fake in low- and high-fidelity exercises. Additionally, the TPB showed good predictive validity for faking in a low-fidelity exercise, yet not for faking in high-fidelity exercises.

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