scholarly journals Tissue-specific mutation accumulation in human adult stem cells during life

Nature ◽  
2016 ◽  
Vol 538 (7624) ◽  
pp. 260-264 ◽  
Author(s):  
Francis Blokzijl ◽  
Joep de Ligt ◽  
Myrthe Jager ◽  
Valentina Sasselli ◽  
Sophie Roerink ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adult Stem Cells ◽  
Mutation Accumulation ◽  
Tissue Specific ◽  
Specific Mutation ◽  
Human Adult

Measuring mutation accumulation in single human adult stem cells by whole-genome sequencing of organoid cultures

2017 ◽  
Vol 13 (1) ◽  
pp. 59-78 ◽  
Author(s):  
Myrthe Jager ◽  
Francis Blokzijl ◽  
Valentina Sasselli ◽  
Sander Boymans ◽  
Roel Janssen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Adult Stem Cells ◽  
Mutation Accumulation ◽  
Whole Genome ◽  
Human Adult ◽  
Organoid Cultures

Nanoscale definition of substrate materials to direct human adult stem cells towards tissue specific populations

2009 ◽  
Vol 21 (3) ◽  
pp. 1021-1029 ◽  
Author(s):  
Judith M. Curran ◽  
Rui Chen ◽  
Robert Stokes ◽  
Eleanor Irvine ◽  
Duncan Graham ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adult Stem Cells ◽  
Tissue Specific ◽  
Human Adult ◽  
Definition Of

scholarly journals Adult tissue-resident stem cells—fact or fiction?

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Deepa Bhartiya
Keyword(s):  
Stem Cells ◽  
Single Cell ◽  
Adult Stem Cells ◽  
Cell Types ◽  
Specific Cell ◽  
Tissue Specific ◽  
Cardiac Tissues ◽  
Adult Tissues ◽  
Resident Stem Cells ◽  
Abundant Cytoplasm

AbstractLife-long tissue homeostasis of adult tissues is supposedly maintained by the resident stem cells. These stem cells are quiescent in nature and rarely divide to self-renew and give rise to tissue-specific “progenitors” (lineage-restricted and tissue-committed) which divide rapidly and differentiate into tissue-specific cell types. However, it has proved difficult to isolate these quiescent stem cells as a physical entity. Recent single-cell RNAseq studies on several adult tissues including ovary, prostate, and cardiac tissues have not been able to detect stem cells. Thus, it has been postulated that adult cells dedifferentiate to stem-like state to ensure regeneration and can be defined as cells capable to replace lost cells through mitosis. This idea challenges basic paradigm of development biology regarding plasticity that a cell enters point of no return once it initiates differentiation. The underlying reason for this dilemma is that we are putting stem cells and somatic cells together while processing for various studies. Stem cells and adult mature cell types are distinct entities; stem cells are quiescent, small in size, and with minimal organelles whereas the mature cells are metabolically active and have multiple organelles lying in abundant cytoplasm. As a result, they do not pellet down together when centrifuged at 100–350g. At this speed, mature cells get collected but stem cells remain buoyant and can be pelleted by centrifuging at 1000g. Thus, inability to detect stem cells in recently published single-cell RNAseq studies is because the stem cells were unknowingly discarded while processing and were never subjected to RNAseq. This needs to be kept in mind before proposing to redefine adult stem cells.

Apple ethanol extract promotes proliferation of human adult stem cells, which involves the regenerative potential of stem cells

2016 ◽  
Vol 36 (9) ◽  
pp. 925-936 ◽  
Author(s):  
Jienny Lee ◽  
Moon Sam Shin ◽  
Mi Ok Kim ◽  
Sunghee Jang ◽  
Sae Woong Oh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adult Stem Cells ◽  
Ethanol Extract ◽  
Human Adult

scholarly journals Human Adult Stem Cells Maintain a Constant Phenotype Profile Irrespective of Their Origin, Basal Media, and Long Term Cultures

2015 ◽  
Vol 2015 ◽  
pp. 1-29 ◽  
Author(s):  
Indumathi Somasundaram ◽  
Rashmi Mishra ◽  
Harikrishnan Radhakrishnan ◽  
Rajkumar Sankaran ◽  
Venkata Naga Srikanth Garikipati ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adult Stem Cells ◽  
Expression Profiles ◽  
Subcutaneous Fat ◽  
Adult Stem Cell ◽  
Phenotypic Marker ◽  
Human Adult ◽  
Cell Based Therapy ◽  
Basal Media

The study aims to identify the phenotypic marker expressions of different human adult stem cells derived from, namely, bone marrow, subcutaneous fat, and omentum fat, cultured in different media, namely, DMEM-Low Glucose, Alpha-MEM, DMEM-F12 and DMEM-KO and under long term culture conditions (>P20). We characterized immunophenotype by using various hematopoietic, mesenchymal, endothelial markers, and cell adhesion molecules in the long term cultures (Passages-P1, P3, P5, P9, P12, P15, and P20.) Interestingly, data revealed similar marker expression profiles irrespective of source, basal media, and extensive culturing. This demonstrates that all adult stem cell sources mentioned in this study share similar phenotypic marker and all media seem appropriate for culturing these sources. However, a disparity was observed in the markers such as CD49d, CD54, CD117, CD29, and CD106, thereby warranting further research on these markers. Besides the aforesaid objective, it is understood from the study that immunophenotyping acts as a valuable tool to identify inherent property of each cell, thereby leading to a valuable cell based therapy.

scholarly journals The Therapeutic Effect of Human Adult Stem Cells Derived from Adipose Tissue in Endotoxemic Rat Model

2013 ◽  
Vol 10 (1) ◽  
pp. 8-18 ◽  
Author(s):  
Soyoung Shin ◽  
Yonggoo Kim ◽  
Sikyoung Jeong ◽  
Sungyoup Hong ◽  
Insoo Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Therapeutic Effect ◽  
Rat Model ◽  
Adult Stem Cells ◽  
Human Adult

scholarly journals The Wnt Signaling Inhibitor Dickkopf-1 Is Required for Reentry into the Cell Cycle of Human Adult Stem Cells from Bone Marrow

2003 ◽  
Vol 278 (30) ◽  
pp. 28067-28078 ◽  
Author(s):  
Carl A. Gregory ◽  
Harpreet Singh ◽  
Anthony S. Perry ◽  
Darwin J. Prockop
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Bone Marrow ◽  
Wnt Signaling ◽  
Adult Stem Cells ◽  
Human Adult ◽  
Dickkopf 1
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