scholarly journals In vivo imaging of Treg cells providing immune privilege to the haematopoietic stem-cell niche

Nature ◽  
2011 ◽  
Vol 474 (7350) ◽  
pp. 216-219 ◽  
Author(s):  
Joji Fujisaki ◽  
Juwell Wu ◽  
Alicia L. Carlson ◽  
Lev Silberstein ◽  
Prabhakar Putheti ◽  
...  
Keyword(s):  
Stem Cell ◽  
In Vivo Imaging ◽  
Stem Cell Niche ◽  
Treg Cells ◽  
Immune Privilege ◽  
Haematopoietic Stem Cell ◽  
Cell Niche

scholarly journals The bone metastasis niche in breast cancer: potential overlap with the haematopoietic stem cell niche in vivo

2019 ◽  
Vol 17 ◽  
pp. 100244 ◽  
Author(s):  
Gloria Allocca ◽  
Russell Hughes ◽  
Ning Wang ◽  
Hannah K Brown ◽  
Penelope D Ottewell ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cell ◽  
Bone Metastasis ◽  
Stem Cell Niche ◽  
Haematopoietic Stem Cell ◽  
Cell Niche

Quantification of stem cell / niche interactions by coupling in vivo imaging and in silico simulation

2013 ◽  
Vol 41 (8) ◽  
pp. S31
Author(s):  
Ingo Roeder ◽  
Axel Krinner ◽  
Nico Scherf ◽  
Marc Scott ◽  
Narges Rashidi ◽  
...  
Keyword(s):  
Stem Cell ◽  
In Vivo Imaging ◽  
In Silico ◽  
Stem Cell Niche ◽  
In Silico Simulation ◽  
Cell Niche

scholarly journals Human aplastic anaemia-derived mesenchymal stromal cells form functional haematopoietic stem cell niche in vivo

2016 ◽  
Vol 179 (4) ◽  
pp. 669-673 ◽  
Author(s):  
Ilaria M. Michelozzi ◽  
Alice Pievani ◽  
Fabio Pagni ◽  
Laura Antolini ◽  
Marta Verna ◽  
...  
Keyword(s):  
Stem Cell ◽  
Aplastic Anaemia ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Stem Cell Niche ◽  
Haematopoietic Stem Cell ◽  
Cell Niche

MESENCHYMAL TNFR1 EXPRESSION PRESERVES THE COLONIC EPITHELIAL STEM CELL NICHE

2021 ◽  
Vol 27 (Supplement_1) ◽  
pp. S7-S8
Author(s):  
Safina Gadeock ◽  
Cambrian Liu ◽  
Brent Polk
Keyword(s):  
Stem Cell ◽  
Stem Cell Niche ◽  
Mesenchymal Cells ◽  
Epithelial Stem Cells ◽  
Epithelial Stem Cell ◽  
Long Term Treatment ◽  
Lgr5 Expression ◽  
Cell Niche

Abstract Tumor necrosis factor (TNF) is a highly expressed cytokine in inflammatory bowel disease (IBD). Although TNF can induce colonic epithelial dysfunction and apoptosis, recent studies suggest that TNF signalling promotes epithelial wound repair and stem cell function. Here we investigated the role of TNF receptor 1 (TNFR1) in mediating TNF’s effects on colonic epithelial stem cells, integral to mucosal healing in colitis. We demonstrate that Tnfr1-/- mice exhibit loss in Lgr5 expression (-52%, p<0.02; N=6) compared to wildtype (WT) controls. However, the opposite result was found in vitro, wherein murine Tnfr1-/- colonoids demonstrated a significant increase in Lgr5 expression (66%, p<0.007; N=6) compared to WT colonoids. Similarly, human colonoids treated with an anti-TNFR1 antibody also demonstrated an increase in Lgr5 expression, relative to IgG controls. To resolve the contradiction in the in vivo versus in vitro environment, we hypothesized that mesenchymal TNFR1 expression regulates the epithelial stem cell niche. To determine the relationships between these cell types, we co-cultured WT or Tnfr1-/- colonoids with WT or Tnfr1-/- colonic myofibroblasts (CMFs). We found that epithelial Lgr5 expression was significantly higher (by 52%, p<0.05; N=3) when co-cultured with WT compared to TNFR1-/- myofibroblasts. The loss of TNFR1 expression in vivo increases the number of αSMA+ mesenchymal cells by nearly 56% (N=6) but considerably reduces the pericryptal PDGFRα+ cells, suggesting modifications in mesenchymal populations that contribute to the epithelial stem cell niche. Functionally, primary Tnfr1-/--CMFs displayed PI3k (p<0.001; N=3) and MAPK (p<0.01; N=3)-dependent increases in migration, proliferation, and differentiation, but RNA profiling demonstrated by diminished levels of stem cell niche factors, Rspo3 (-80%, p<0.0001; N=6) and Wnt2b (-63%, p<0.008; N=6) compared to WT-CMFs. Supplementation with 50ng recombinant Rspo3 for 5 d to Lgr5-GFP organoids co-cultured with TNFR1-/--CMFs restored Lgr5 expression to wildtype levels. Therefore, TNFR1-mediated TNF signalling in mesenchymal cells promotes their ability to support an epithelial stem cell niche. These results should motivate future studies of the stem cell niche in the context of long-term treatment with anti-TNF therapies.

scholarly journals Lymphatic vessels interact dynamically with the hair follicle stem cell niche during skin regeneration in vivo

2019 ◽  
Vol 38 (19) ◽  
Author(s):  
Daniel Peña‐Jimenez ◽  
Silvia Fontenete ◽  
Diego Megias ◽  
Coral Fustero‐Torre ◽  
Osvaldo Graña‐Castro ◽  
...  
Keyword(s):  
Stem Cell ◽  
Hair Follicle ◽  
Stem Cell Niche ◽  
Lymphatic Vessels ◽  
Skin Regeneration ◽  
Hair Follicle Stem Cell ◽  
Cell Niche
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