scholarly journals The amphioxus genome and the evolution of the chordate karyotype

Nature ◽  
2008 ◽  
Vol 453 (7198) ◽  
pp. 1064-1071 ◽  
Author(s):  
Nicholas H. Putnam ◽  
Thomas Butts ◽  
David E. K. Ferrier ◽  
Rebecca F. Furlong ◽  
Uffe Hellsten ◽  
...  
Keyword(s):  
Amphioxus Genome

scholarly journals Application of CRISPR/Cas9 Nuclease in Amphioxus Genome Editing

Genes ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 1311
Author(s):  
Liuru Su ◽  
Chenggang Shi ◽  
Xin Huang ◽  
Yiquan Wang ◽  
Guang Li
Keyword(s):  
Animal Model ◽  
Thermal Treatment ◽  
Genome Editing ◽  
Phylogenetic Position ◽  
Transcription Activator ◽  
Cell Stage ◽  
System A ◽  
Cas9 Nuclease ◽  
Cas9 Protein ◽  
Amphioxus Genome

The cephalochordate amphioxus is a promising animal model for studying the origin of vertebrates due to its key phylogenetic position among chordates. Although transcription activator-like effector nucleases (TALENs) have been adopted in amphioxus genome editing, its labor-intensive construction of TALEN proteins limits its usage in many laboratories. Here we reported an application of the CRISPR/Cas9 system, a more amenable genome editing method, in this group of animals. Our data showed that while co-injection of Cas9 mRNAs and sgRNAs into amphioxus unfertilized eggs caused no detectable mutations at targeted loci, injections of Cas9 mRNAs and sgRNAs at the two-cell stage, or of Cas9 protein and sgRNAs before fertilization, can execute efficient disruptions of targeted genes. Among the nine tested sgRNAs (targeting five genes) co-injected with Cas9 protein, seven introduced mutations with efficiency ranging from 18.4% to 90% and four caused specific phenotypes in the injected embryos. We also demonstrated that monomerization of sgRNAs via thermal treatment or modifying the sgRNA structure could increase mutation efficacies. Our study will not only promote application of genome editing method in amphioxus research, but also provide valuable experiences for other organisms in which the CRISPR/Cas9 system has not been successfully applied.

scholarly journals The amphioxus genome provides unique insight into the evolution of immunity

2012 ◽  
Vol 11 (2) ◽  
pp. 167-176 ◽  
Author(s):  
L. J. Dishaw ◽  
R. N. Haire ◽  
G. W. Litman
Keyword(s):  
Amphioxus Genome ◽  
Evolution Of Immunity ◽  
Insight Into

scholarly journals Insights from the amphioxus genome on the origin of vertebrate neural crest

2008 ◽  
Vol 18 (7) ◽  
pp. 1127-1132 ◽  
Author(s):  
J.-K. Yu ◽  
D. Meulemans ◽  
S. J. McKeown ◽  
M. Bronner-Fraser
Keyword(s):  
Neural Crest ◽  
Amphioxus Genome

A proposal to sequence the amphioxus genome submitted to the joint genome institute of the US department of energy

2003 ◽  
Vol 300B (1) ◽  
pp. 5-22 ◽  
Author(s):  
Jeremy J. Gibson-Brown ◽  
Kazutoyo Osoegawa ◽  
John D. McPherson ◽  
Robert H. Waterston ◽  
Pieter J. de Jong ◽  
...  
Keyword(s):  
Department Of Energy ◽  
Joint Genome Institute ◽  
The Us ◽  
Amphioxus Genome

scholarly journals The amphioxus genome illuminates vertebrate origins and cephalochordate biology

2008 ◽  
Vol 18 (7) ◽  
pp. 1100-1111 ◽  
Author(s):  
L. Z. Holland ◽  
R. Albalat ◽  
K. Azumi ◽  
E. Benito-Gutierrez ◽  
M. J. Blow ◽  
...  
Keyword(s):  
Amphioxus Genome

scholarly journals How much does the amphioxus genome represent the ancestor of chordates?

2012 ◽  
Vol 11 (2) ◽  
pp. 89-95 ◽  
Author(s):  
A. Louis ◽  
H. Roest Crollius ◽  
M. Robinson-Rechavi
Keyword(s):  
Amphioxus Genome

The amphioxus genome enlightens the evolution of the thyroid hormone signaling pathway

2008 ◽  
Vol 218 (11-12) ◽  
pp. 667-680 ◽  
Author(s):  
Mathilde Paris ◽  
Frédéric Brunet ◽  
Gabriel V. Markov ◽  
Michael Schubert ◽  
Vincent Laudet
Keyword(s):  
Thyroid Hormone ◽  
Signaling Pathway ◽  
Hormone Signaling ◽  
Amphioxus Genome
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