A single class of olfactory neurons mediates behavioural responses to a Drosophila sex pheromone

Nature ◽  
2007 ◽  
Vol 446 (7135) ◽  
pp. 542-546 ◽  
Author(s):  
Amina Kurtovic ◽  
Alexandre Widmer ◽  
Barry J. Dickson
Keyword(s):  
Sex Pheromone ◽  
Single Class ◽  
Olfactory Neurons ◽  
Behavioural Responses

scholarly journals Identification and characterisation of female released sex pheromone components of jute semilooper, Anomis sabulifera Guenee (Lepidoptera: Noctuidae)

2021 ◽  
Vol 42 (2) ◽  
pp. 254-264
Author(s):  
V.R. Babu ◽  
◽  
S. Satpathy ◽  
B.V.S. Reddy ◽  
◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Sex Pheromone ◽  
Wind Tunnel ◽  
Pheromone Gland ◽  
Behavioural Responses ◽  
Gland Extract ◽  
Female Pheromone ◽  
Antennal Response ◽  
Pheromone Components ◽  
Lepidoptera Noctuidae

Aim: Identification and characterization of female released sex pheromone components of jute semilooper, Anomis sabulifera Guenee (Lepidoptera: Noctuidae) from female pheromone gland extracts. Methodology: Electroantennogram (EAG) was carried for studying the antennal response; Gas Chromatography coupled with Electro antenna Detector (GC-EAD) was conducted for studying the antennal response of eluted compounds from female pheromone gland extract; Gas Chromatography and Mas Spectrophotometry (GC-MS) was conducted for characterization or getting complete profile of compounds present in the female pheromone gland extract based on retention times. Wind tunnel assay was conducted for studying the behavioural responses of eluted compounds from the female pheromone gland extract. Results: GC-MS profile of female pheromone gland extract revealed that the GC-EAD active region constituted (6Z,9Z)-heneicosadiene, (3Z,6Z,9Z)-heneicosatriene as active compounds. Preliminary wind tunnel studies for olfactory and behavioural responses showed blend of (6Z,9Z)-heneicosadiene (3 parts) + (3Z,6Z,9Z)-heneicosatriene (1 part) enticed 60% male adults. Interpretation: (6Z,9Z)-heneicosadiene and (3Z,6Z,9Z)-heneicosatriene are likely to be active pheromone components present in female sex pheromone glands. Blending of these two compounds in precise ratio can enhance the effectiveness of pheromone and can be used as effective strategy in jute IPM. Key words: Anomis sabulifera, Jute semilooper, Noctuidae, Sex pheromone, (6Z,9Z)-heneicosadiene, (3Z,6Z,9Z)-heneicosatriene

scholarly journals Targeted disruption of a single sex pheromone receptor gene completely abolishes in vivo pheromone response in the silkmoth

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Takeshi Sakurai ◽  
Hidefumi Mitsuno ◽  
Akihisa Mikami ◽  
Keiro Uchino ◽  
Masashi Tabuchi ◽  
...  
Keyword(s):  
Sex Pheromone ◽  
Sexual Behaviour ◽  
Receptor Gene ◽  
Transcription Activator ◽  
Pheromone Receptor ◽  
Behavioural Responses ◽  
Sensilla Trichodea ◽  
Moth Species ◽  
Species Specific

Abstract Male moths use species-specific sex pheromones to identify and orientate toward conspecific females. Odorant receptors (ORs) for sex pheromone substances have been identified as sex pheromone receptors in various moth species. However, direct in vivo evidence linking the functional role of these ORs with behavioural responses is lacking. In the silkmoth, Bombyx mori, female moths emit two sex pheromone components, bombykol and bombykal, but only bombykol elicits sexual behaviour in male moths. A sex pheromone receptor BmOR1 is specifically tuned to bombykol and is expressed in specialized olfactory receptor neurons (ORNs) in the pheromone sensitive long sensilla trichodea of male silkmoth antennae. Here, we show that disruption of the BmOR1 gene, mediated by transcription activator-like effector nucleases (TALENs), completely removes ORN sensitivity to bombykol and corresponding pheromone-source searching behaviour in male moths. Furthermore, transgenic rescue of BmOR1 restored normal behavioural responses to bombykol. Our results demonstrate that BmOR1 is required for the physiological and behavioural response to bombykol, demonstrating that it is the receptor that mediates sex pheromone responses in male silkmoths. This study provides the first direct evidence that a member of the sex pheromone receptor family in moth species mediates conspecific sex pheromone information for sexual behaviour.

scholarly journals An Insecticide Further Enhances Experience-Dependent Increased Behavioural Responses to Sex Pheromone in a Pest Insect

PLoS ONE ◽  
2016 ◽  
Vol 11 (11) ◽  
pp. e0167469 ◽  
Author(s):  
Antoine Abrieux ◽  
Amel Mhamdi ◽  
Kaouther K. Rabhi ◽  
Julie Egon ◽  
Stéphane Debernard ◽  
...  
Keyword(s):  
Sex Pheromone ◽  
Pest Insect ◽  
Behavioural Responses

scholarly journals Most primary olfactory neurons have individually neutral effects on behavior

2021 ◽  
Author(s):  
Tayfun Tumkaya ◽  
Safwan Burhanudin ◽  
Asghar Khalilnezhad ◽  
James Stewart ◽  
Hyungwon Choi ◽  
...  
Keyword(s):  
Sensory Neurons ◽  
Olfactory Receptors ◽  
Olfactory Receptor Neuron ◽  
Behavioral Effects ◽  
Olfactory Sensory Neurons ◽  
Single Class ◽  
Olfactory Neurons ◽  
Complex Interactions ◽  
Olfactory Systems ◽  
Behavioral Influences

Animals use olfactory receptors to navigate mates, food, and danger. However, for complex olfactory systems, it is unknown what proportion of primary olfactory sensory neurons can individually drive avoidance or attraction. Similarly, the rules that govern behavioral responses to receptor combinations are unclear. We used optogenetic analysis in Drosophila to map the behavior elicited by olfactory-receptor neuron (ORN) classes: just one-fifth of ORN-types drove either avoidance or attraction. Although wind and hunger are closely linked to olfaction, neither had much effect on single-class responses. Several pooling rules have been invoked to explain how ORN types combine their behavioral influences; we activated two-way combinations and compared patterns of single- and double-ORN responses: these comparisons were inconsistent with simple pooling. We conclude that the majority of primary olfactory sensory neurons have neutral behavioral effects individually, but participate in broad, odor-elicited ensembles with potent behavioral effects arising from complex interactions.

scholarly journals Sex Pheromone of the Introduced Pine Sawfly, Diprion similis, Revisited to Define a Useful Monitoring Lure: Deviating Chiral Composition and Behavioural Responses Compared to Earlier Reports

Insects ◽  
2021 ◽  
Vol 12 (10) ◽  
pp. 886
Author(s):  
Olle Anderbrant ◽  
D. Barry Lyons ◽  
Joakim Bång ◽  
Erik Hedenström ◽  
Hans-Erik Högberg
Keyword(s):  
Sex Pheromone ◽  
Synergistic Effects ◽  
Field Tests ◽  
Pheromone Component ◽  
Main Compound ◽  
Behavioural Responses ◽  
Pine Sawfly ◽  
Monitoring Programs ◽  
Diprion Similis ◽  
Full Scan

Extracts of Diprion similis females contained about 15 ng of the sex pheromone precursor 3,7-dimethylpentadecan-2-ol per female. After derivatisation with (S)-2-acetoxypropanoyl chloride, we found that the major stereoisomer in the extract was (2S,3R,7R)-3,7-dimethylpentadecan-2-ol. Small amounts of other stereoisomers of 3,7-dimethylpentadecan-2-ol were also identified in the extract, namely 1% of (2R,3S,7S), 0.3% (2R,3R,7R) and 0.4% of (2R,3R,7S). An unknown fifth substance showed a very similar spectrum to 3,7-dimethylpentadecan-2-ol, both in SIM and full scan mode. None of the earlier suggested behavioural synergistic isomers ((2S,3S,7S), (2S,3S,7R) and (2S,3R,7S)) were detected in the extracts. In field tests in Ontario, Canada, the earlier identified main pheromone component, viz. the propanoate of (2S,3R,7R)-3,7-dimethylpentadecan-2-ol, was tested alone and in combination with other stereoisomers, earlier reported to be synergistic. No synergistic effects were detected and the threo four-isomer blend was as attractive as the pure main compound. Thus, one of the few examples of a diprionid sawfly using more than one substance in its sex pheromone could not be confirmed. The results also suggest that monitoring programs can use the more easily synthesized threo-blend without losing efficiency. Furthermore, the study suggests that other diprionid pheromones may benefit from a reinvestigation, to clarify possible synergistic effects of stereoisomers.

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