scholarly journals Computational redesign of endonuclease DNA binding and cleavage specificity

Nature ◽  
2006 ◽  
Vol 441 (7093) ◽  
pp. 656-659 ◽  
Author(s):  
Justin Ashworth ◽  
James J. Havranek ◽  
Carlos M. Duarte ◽  
Django Sussman ◽  
Raymond J. Monnat ◽  
...  
Keyword(s):  
Dna Binding ◽  
Cleavage Specificity ◽  
Dna Binding And Cleavage

Synthesis, Structural, DNA Binding and Cleavage Studies of Cu(II) Complexes Containing Benzothiazole Cored Schiff Bases

2016 ◽  
Vol 26 (6) ◽  
pp. 2151-2163 ◽  
Author(s):  
Somapangu Tejaswi ◽  
Marri Pradeep Kumar ◽  
Aveli Rambabu ◽  
Narendrula Vamsikrishna ◽  
Shivaraj
Keyword(s):  
Dna Binding ◽  
Schiff Bases ◽  
Dna Binding And Cleavage

scholarly journals Cas12a is a dynamic and precise RNA-guided nuclease without off-target activity on λ-DNA

2021 ◽  
Author(s):  
Bijoya Paul ◽  
Loic Chaubet ◽  
Emma Verver ◽  
Guillermo Montoya
Keyword(s):  
Dna Binding ◽  
Genome Editing ◽  
Optical Tweezers ◽  
Target Site ◽  
Target Dna ◽  
Multiple Binding ◽  
Target Sites ◽  
Dna Binding And Cleavage ◽  
Target Activity ◽  
Insight Into

Cas12a is an RNA-guided endonuclease that is emerging as a powerful genome-editing tool. Here we combined optical tweezers with fluorescence to monitor Cas12a binding onto λ-DNA, providing insight into its DNA binding and cleavage mechanisms. At low forces Cas12a binds DNA specifically with two off-target sites, while at higher forces numerous binding events appear driven by the mechanical distortion of the DNA and partial matches to the crRNA. Despite the multiple binding events, cleavage is only observed on the target site at low forces, when the DNA is flexible. Activity assays show that the preferential off-target sites are not cleaved, and the λ-DNA is severed at the target site. This precision is also observed in Cas12a variants where the specific dsDNA and the unspecific ssDNA cleavage are dissociated or nick the target DNA. We propose that Cas12a and its variants are precise endonucleases that efficiently scan the DNA for its target but only cleave the selected site in the λ-DNA.

Tris-chelated complexes of nickel(II) with bipyridine derivatives: DNA binding and cleavage, BSA binding, molecular docking, and cytotoxicity

2019 ◽  
Vol 37 (15) ◽  
pp. 3887-3904 ◽  
Author(s):  
Marzieh Anjomshoa ◽  
Masoud Torkzadeh-Mahani ◽  
Mehdi Sahihi ◽  
Corrado Rizzoli ◽  
Mehdi Ansari ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Dna Binding ◽  
Bsa Binding ◽  
Dna Binding And Cleavage

scholarly journals Flow cytometric analysis of DNA binding and cleavage by cell surface-displayed homing endonucleases

2007 ◽  
Vol 35 (8) ◽  
pp. 2748-2758 ◽  
Author(s):  
Petra Volná ◽  
Jordan Jarjour ◽  
Sarah Baxter ◽  
Steve R. Roffler ◽  
Raymond J. Monnat ◽  
...  
Keyword(s):  
Dna Binding ◽  
Cell Surface ◽  
Flow Cytometric Analysis ◽  
Homing Endonucleases ◽  
Flow Cytometric ◽  
Dna Binding And Cleavage
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