Structure of the inositol 1,4,5-trisphosphate receptor binding core in complex with its ligand

Nature ◽  
2002 ◽  
Vol 420 (6916) ◽  
pp. 696-700 ◽  
Author(s):  
Ivan Bosanac ◽  
Jean-René Alattia ◽  
Tapas K. Mal ◽  
Jenny Chan ◽  
Susanna Talarico ◽  
...  
Keyword(s):  
Receptor Binding ◽  
Inositol 1,4,5 Trisphosphate ◽  
Binding Core ◽  
Trisphosphate Receptor

scholarly journals Tespa1 is a novel inositol 1,4,5-trisphosphate receptor binding protein in T and B lymphocytes

FEBS Open Bio ◽  
2012 ◽  
Vol 2 (1) ◽  
pp. 255-259 ◽  
Author(s):  
Hiroshi Matsuzaki ◽  
Takahiro Fujimoto ◽  
Takeharu Ota ◽  
Masahiro Ogawa ◽  
Toshiyuki Tsunoda ◽  
...  
Keyword(s):  
B Lymphocytes ◽  
Receptor Binding ◽  
Binding Protein ◽  
T And B Lymphocytes ◽  
Inositol 1,4,5 Trisphosphate ◽  
Trisphosphate Receptor ◽  
Receptor Binding Protein

Maturation of inositol 1,4,5-trisphosphate receptor binding in rabbit tracheal smooth muscle

1992 ◽  
Vol 263 (4) ◽  
pp. L501-L505 ◽  
Author(s):  
C. M. Schramm ◽  
S. T. Chuang ◽  
M. M. Grunstein
Keyword(s):  
Smooth Muscle ◽  
Receptor Binding ◽  
Tracheal Smooth Muscle ◽  
Binding Characteristics ◽  
Adult Tissues ◽  
Inositol 1,4,5 Trisphosphate ◽  
Dependent Inhibition ◽  
Dose Dependent Inhibition ◽  
Hill Coefficients ◽  
Trisphosphate Receptor

The mechanisms underlying maturational changes in agonist-mediated airway contractility remain to be identified. Since the signal transduction process coupled to airway contraction involves the Ca(2+)-mobilizing action of the second messenger, inositol 1,4,5-trisphosphate [Ins(1,4,5)P3], the present study examined 1) whether Ins(1,4,5)P3 binding to its intracellular receptor varies with age in rabbit tracheal smooth muscle (TSM), and 2) whether Ca2+ exerts a modulatory effect on Ins(1,4,5)P3 receptor binding that is age dependent. [3H]Ins(1,4,5)P3 binding was assayed in crude TSM membrane preparations isolated from 2-week-old and adult rabbits. Monophasic Scatchard plots were obtained, reflecting a single binding site, with Hill coefficients of 0.988–0.996. The mean +/- SE values for receptor density (Bmax) and binding affinity [i.e., the dissociation constant (Kd)] were similar in the adult and immature tissues, wherein Bmax = 211 +/- 6 238 +/- 60 fmol/mg protein, respectively; and Kd = 14.1 +/- 0.2 and 11.6 +/- 1.2 nM, respectively. Addition of Ca2+ (10(-8)-10(-3) M) significantly modulated Ins(1,4,5)P3 binding, with opposing maturational effects. In adult tissues, Ca2+ produced dose-dependent inhibition of Ins(1,4,5)P3 binding to 59.5% control, whereas Ins(1,4,5)P3 binding in response to Ca2+ was significantly enhanced in the 2-week-old tissues to 183% control. Collectively, these observations demonstrate that the inherent basal binding characteristics of the Ins(1,4,5)P3 receptor are similar in maturing TSM, but that Ca2+ exerts opposite modulatory actions on Ins(1,4,5)P3 receptor binding in immature and adult tissues.(ABSTRACT TRUNCATED AT 250 WORDS)

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