Protective role of phospholipid oxidation products in endotoxin-induced tissue damage

Nature ◽  
2002 ◽  
Vol 419 (6902) ◽  
pp. 77-81 ◽  
Author(s):  
Valery N. Bochkov ◽  
Alexandra Kadl ◽  
Joakim Huber ◽  
Florian Gruber ◽  
Bernd R. Binder ◽  
...  
Keyword(s):  
Tissue Damage ◽  
Protective Role ◽  
Oxidation Products

The Protective Role of Selenium in AFB1-Induced Tissue Damage and Cell Cycle Arrest in Chicken’s Bursa of Fabricius

2018 ◽  
Vol 185 (2) ◽  
pp. 486-496 ◽  
Author(s):  
Ping Hu ◽  
Zhicai Zuo ◽  
Fengyuan Wang ◽  
Xi Peng ◽  
Ke Guan ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Tissue Damage ◽  
Protective Role ◽  
Bursa Of Fabricius ◽  
Cycle Arrest

Anti-inflammatory properties of probiotic bacteria on Salmonella-induced IL-8 synthesis in enterocyte-like Caco-2 cells

2010 ◽  
Vol 1 (2) ◽  
pp. 121-130 ◽  
Author(s):  
J. Malago ◽  
P. Tooten ◽  
J.F. Koninkx
Keyword(s):  
Tissue Damage ◽  
Protective Role ◽  
Bifidobacterium Bifidum ◽  
Probiotic Bacterium ◽  
Hsp70 Expression ◽  
Bacterial Cells ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Inflammatory Reactions ◽  
Mediated Reduction

Invasion of the gut by pathogenic Salmonella leads to production of IL-8 that initiates inflammatory reactions to combat the bacterium. However, its persistent production causes tissue damage and interventions that suppress IL-8 production prevent tissue damage. We hypothesised that probiotics could mediate their benefits via inhibition of IL-8 synthesis. Caco-2 cells were infected with probiotic Bifidobacterium infantis W52, Lactobacillus casei W56, Lactococcus lactis W58, Lactobacillus acidophilus W70, Bifidobacterium bifidum W23, or Lactobacillus salivarius W24 or pathogenic Salmonella enterica serovar Enteritidis 857 at 0, 0.2, 1, 2, 10, 20, 100 or 200 bacterial cells/Caco-2 cell for 1 hour. Cells were also exposed to a combination of one probiotic bacterium (200 bacterial cells/Caco-2 cell) and the graded numbers of Salmonella as either co-incubation (1 hour) or pre-incubation of the probiotic bacterium (1 hour) followed by Salmonella (1 hour). The cells recovered for 2 or 24 hours. IL-8 and Hsp70 were determined by ELISA and Western blot respectively. Both probiotics and Salmonella induced a dose- and time-dependent synthesis of IL-8 but probiotics induced far lower IL-8 levels than Salmonella. The Salmonella-induced IL-8 was significantly suppressed by B. infantis W52, L. casei W56 and L. lactis W58 at low numbers of Salmonella (0.2 to 20 bacterial cells/Caco-2 cell) and within 2 hours of recovery. The observed probiotic-mediated reduction in IL-8 secretion was transient, and lost after a few hours. In addition, these three probiotics induced a significant increase in Hsp70 expression while L. acidophilus W70, B. bifidum W23 and L. salivarius W24 induced a weak Hsp70 expression and could not suppress the Salmonella-induced IL-8 synthesis. We conclude that suppression of Salmonella-induced IL-8 synthesis by Caco-2 cells is exhibited by probiotics that induce expression of Hsp70, suggesting that the protective role of probiotics could be mediated, at least in part, via Hsp70 expression. This suppression is limited to a low number of infecting pathogenic Salmonella.

scholarly journals The subchronic exposure to malathion, an organophosphate pesticide, causes lipid peroxidation, oxidative stress, and tissue damage in rats: the protective role of resveratrol

2018 ◽  
Vol 7 (3) ◽  
pp. 503-512 ◽  
Author(s):  
Erten Akbel ◽  
Damla Arslan-Acaroz ◽  
Hasan Huseyin Demirel ◽  
Ismail Kucukkurt ◽  
Sinan Ince
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Tissue Damage ◽  
Protective Role ◽  
Organophosphate Pesticide ◽  
Subchronic Exposure

The present study was planned to evaluate the protective role of resveratrol (Res) against subchronic malathion exposure in rats over four weeks.

Abstract 139: Loss of Thymosin b4 Exacerbates Renal and Cardiac Damage in Angiotensin-II Hypertension

Hypertension ◽  
2017 ◽  
Vol 70 (suppl_1) ◽  
Author(s):  
Nitin Kumar ◽  
Tang-Dong Liao ◽  
Cesar Romero ◽  
Mani Maheshwari ◽  
Ed Peterson ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Tissue Damage ◽  
Cardiac Fibrosis ◽  
Organ Damage ◽  
Protective Role ◽  
High Expression ◽  
Ang Ii ◽  
Cardiac Damage ◽  
Filtration Barrier

Angiotensin-II (Ang-II)-induced hypertension is associated with tissue damage and fibrosis in the kidney and heart. Thymosin β4 (Tβ4) regulates cell morphology, inflammation and fibrosis in several organs and administration of exogenous Tβ4 is protective in diabetic nephropathy and unilateral ureteral obstruction model. However, role of endogenous Tβ4 in hypertension-induced organ damage is unknown. We hypothesize that, loss of Tβ4 accelerates renal and cardiac fibrosis and damage in Ang-II hypertension. To test our hypothesis, Tβ4 knockout (Tβ4 -/- ) and wild-type (Tβ4 +/+ ) C57BL/6 mice (n=6-10) were infused continuously for six-weeks with either Ang-II (980 ng/kg/min) or vehicle via osmotic minipumps. All the results are presented in table 1. In Ang-II infusion, systolic blood-pressure were not different between both strains (Table 1). Interestingly, urinary albuminuria was significantly higher in Tβ4 -/- mice compared to Tβ4 +/+ mice by Ang-II. High expression of Tβ4 is found in the glomeruli along with high expression of Nephrin, an important protein in the filtration barrier of the kidney. In Ang-II infusion, nephrin protein expression was greatly reduced in mice deficient of Tβ4, suggesting that loss of nephrin is one of the mechanism for elevated urinary albumin in Tβ4 -/- mice. Additionally, renal fibrosis was higher in Tβ4 -/- mice. We also studied cardiac damage and observed that in Ang-II infusion, cardiac hypertrophy and cardiac fibrosis were much higher in Tβ4 -/- mice. These data indicate that loss of endogenous Tβ4 caused significant tissue damage in the kidney and heart in Ang-II hypertension, suggesting renal and cardiac protective role of this peptide.

scholarly journals Matrix metalloproteinase-9-mediated tissue injury overrides the protective effect of matrix metalloproteinase-2 during colitis

2009 ◽  
Vol 296 (2) ◽  
pp. G175-G184 ◽  
Author(s):  
Pallavi Garg ◽  
Matam Vijay-Kumar ◽  
Lixin Wang ◽  
Andrew T. Gewirtz ◽  
Didier Merlin ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Tissue Damage ◽  
Tissue Injury ◽  
Protective Role ◽  
Matrix Metalloproteinase 2 ◽  
Trinitrobenzene Sulfonic Acid ◽  
Myeloperoxidase Activity ◽  
Double Knockout ◽  
Gut Barrier Function

Matrix metalloproteinases (MMP) play an important role in pathogenesis of inflammatory bowel disease (IBD). Two known gelatinases, MMP-2 and MMP-9, are upregulated during IBD. Epithelial-derived MMP-9 is an important mediator of tissue injury in colitis, whereas MMP-2 protects against tissue damage and maintains gut barrier function. It has been suggested that developing strategies to block MMP-9 activity in the gut might be of benefit to IBD. However, given that MMP-2 and MMP-9 are structurally similar, such approaches would also likely inhibit MMP-2. Thus, to gain insight into outcome of inhibiting both MMP-2 and MMP-9, MMP-2−/−/MMP-9−/− double knockout mice (dKO) lacking both MMP-2 and MMP-9 were used in this study. Three models of murine colitis were used: dextran sodium sulfate (DSS), Salmonella typhimurium (S.T.), and trinitrobenzene sulfonic acid (TNBS). Our data demonstrate that MMP-2 and MMP-9 activities were highly upregulated in wild-type (WT) mice treated with DSS, S.T., or TNBS whereas dKO mice were resistant to the development of colitis. WT mice had extensive inflammation and tissue damage compared with dKO mice as suggested by histological assessment and myeloperoxidase activity. In conclusion, these results suggest an overriding role of MMP-9 in mediating tissue injury compared with the protective role of MMP-2 in development of colitis. Thus inhibition of MMP-9 may be beneficial in treatment of colitis even if resulting in inhibition of MMP-2.

scholarly journals Protective role of curcumin against the toxic effects of cyclophosphamide in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ)Bg9

2012 ◽  
Vol 2 (1) ◽  
pp. 2 ◽  
Author(s):  
Yasir Hasan Siddique
Keyword(s):  
Drosophila Melanogaster ◽  
Tissue Damage ◽  
Protective Role ◽  
Toxic Effects ◽  
Hsp70 Expression ◽  
The Third ◽  
Dose Dependent ◽  
Transgenic Drosophila ◽  
Third Instar Larvae

In the present study the effects of curcumin was studied against the toxic effects induced by 0.025 and 0.050 ml/ml of cyclophosphamide (CP) in the third instar larvae of transgenic <em>Drosophila melanogaster (hsp70-lacZ)Bg9</em> using hsp70 expression and dye exclusion test as a parameter. The exposure of the third instar larvae to 0.025ml/ml of CP along with 1, 5 and 10 mg/ml of curcumin results in the dose dependent significant decrease in the <em>hsp70</em> expression and tissue damage for 12, 24 and 48hr of duration. Similar results were obtained with the exposure of third instar larvae to 0.050 ml/ml of CP along with 1, 5 and 10 mg/ml of curcumin. The selected doses of curcumin i.e. 1, 5 and 10 mg/ml were not toxic but reduced significantly the expression of <em>hsp70</em> and tissue damage induced by CP. The results of the present study suggest that the curcumin has a protective role against the toxic effects of CP in the third instar larvae of transgenic <em>Drosophila melanogaster (hsp70-lacZ)Bg9</em>.

Free radical tissue damage: protective role of antioxidant nutrients 1

1987 ◽  
Vol 1 (6) ◽  
pp. 441-445 ◽  
Author(s):  
Lawrence J. Machlin ◽  
Adrianne Bendich
Keyword(s):  
Free Radical ◽  
Tissue Damage ◽  
Protective Role

scholarly journals Protective Role of Sphingomyelin in Eye Lens Cell Membrane Model against Oxidative Stress

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 276
Author(s):  
Mehdi Ravandeh ◽  
Giulia Coliva ◽  
Heike Kahlert ◽  
Amir Azinfar ◽  
Christiane A. Helm ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Oxidative Damage ◽  
Plasma Treatment ◽  
High Resolution Mass Spectrometry ◽  
Protective Role ◽  
Oxidation Products ◽  
Eye Lens ◽  
Antioxidant Systems ◽  
Lens Cell

In the eye lens cell membrane, the lipid composition changes during the aging process: the proportion of sphingomyelins (SM) increases, that of phosphatidylcholines decreases. To investigate the protective role of the SMs in the lens cell membrane against oxidative damage, analytical techniques such as electrochemistry, high-resolution mass spectrometry (HR-MS), and atomic force microscopy (AFM) were applied. Supported lipid bilayers (SLB) were prepared to mimic the lens cell membrane with different fractions of PLPC/SM (PLPC: 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine). The SLBs were treated with cold physical plasma. A protective effect of 30% and 44% in the presence of 25%, and 75% SM in the bilayer was observed, respectively. PLPC and SM oxidation products were determined via HR-MS for SLBs after plasma treatment. The yield of fragments gradually decreased as the SM ratio increased. Topographic images obtained by AFM of PLPC-bilayers showed SLB degradation and pore formation after plasma treatment, no degradation was observed in PLPC/SM bilayers. The results of all techniques confirm the protective role of SM in the membrane against oxidative damage and support the idea that the SM content in lens cell membrane is increased during aging in the absence of effective antioxidant systems to protect the eye from oxidative damage and to prolong lens transparency.

Protective Role of Ethanolic Extract of Vernonia amygdalina Against Potassium Bromate Induced Tissue Damage in Wistar Rats

2011 ◽  
Vol 11 (1) ◽  
pp. 54-57 ◽  
Author(s):  
S.J. Josiah ◽  
S.C.O. Nwangwu ◽  
A.A. Akintola ◽  
U. Usunobun ◽  
F.S. Oyefule ◽  
...  
Keyword(s):  
Tissue Damage ◽  
Wistar Rats ◽  
Ethanolic Extract ◽  
Protective Role ◽  
Potassium Bromate ◽  
Vernonia Amygdalina
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