scholarly journals Systematic Analysis of Long Noncoding RNAs in the Senescence-accelerated Mouse Prone 8 Brain Using RNA Sequencing

2016 ◽  
Vol 5 ◽  
pp. e343 ◽  
Author(s):  
Shuai Zhang ◽  
Chunxia Qin ◽  
Guoqiong Cao ◽  
Wenfeng Xin ◽  
Chengqiang Feng ◽  
...  
Keyword(s):  
Rna Sequencing ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas ◽  
Systematic Analysis ◽  
Senescence Accelerated Mouse

RNA sequencing identifies two novel liver-specific long noncoding RNAs with potential diagnostic value in hepatocellular carcinoma

2021 ◽  
Author(s):  
Chao Tan ◽  
Xi Zeng ◽  
Meile Mo ◽  
Xiaoyun Ma ◽  
Qiuli Liang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Rna Sequencing ◽  
Reverse Transcription ◽  
Expression Profiles ◽  
Noncoding Rnas ◽  
Area Under The Curve ◽  
Long Noncoding Rnas ◽  
Normal Tissues ◽  
Quantitative Reverse Transcription Pcr ◽  
Reverse Transcription Pcr

Aim: To explore the expression profiles of long noncoding RNAs (lncRNAs) and identify novel lncRNAs as biomarkers for early diagnosis and therapy of hepatocellular carcinoma (HCC). Materials & methods: Expression profiles of lncRNAs and mRNAs in five paired HCC and adjacent normal tissues were obtained by RNA sequencing. Eight lncRNAs, including two novel liver-specific lncRNAs (NONHSAT059247.2 and NONHSAT013897.2), were validated in another 74 pairs of HCC and adjacent normal tissues by quantitative reverse transcription PCR. Results: The results of quantitative reverse transcription PCR showed that NONHSAT252133.1, NONHSAT112116.2 and NONHSAT242657.1 were significantly upregulated in HCC tissues, whereas NONHSAT169790.1, NONHSAT059247.2 and NONHSAT013897.2 were significantly downregulated. Two liver-specific lncRNAs demonstrated excellent diagnostic performance: NONHSAT059247.2 (area under the curve = 0.941, 95% CI: 0.902–0.979, p < 0.0001), NONHSAT013897.2 (area under the curve = 0.944, 95% CI: 0.906–0.983, p < 0.0001). Conclusion: The liver-specific lncRNAs NONHSAT059247.2 and NONHSAT013897.2, may provide new biomarkers for the future study on diagnosis, therapy, and mechanisms of HCC.

scholarly journals Genome‑wide identification of long noncoding RNAs in CCl4‑induced liver fibrosis via RNA sequencing

2018 ◽  
Author(s):  
Zhenghua Gong ◽  
Jialin Tang ◽  
Tianxin Xiang ◽  
Jiayu Lin ◽  
Chaowen Deng ◽  
...  
Keyword(s):  
Liver Fibrosis ◽  
Rna Sequencing ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas ◽  
Genome Wide

scholarly journals RNA sequencing analysis of altered expression of long noncoding RNAs associated with Schistosoma japonicum infection in the murine liver and spleen

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Tianqi Xia ◽  
Bikash Ranjan Giri ◽  
Jingyi Liu ◽  
Pengfei Du ◽  
Xue Li ◽  
...  
Keyword(s):  
Rna Sequencing ◽  
Schistosoma Japonicum ◽  
Target Genes ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas ◽  
Differentially Expressed ◽  
Protein Coding ◽  
Altered Expression ◽  
Protein Coding Genes

Abstract Background Schistosomiasis is a chronic, debilitating infectious disease caused by members of the genus Schistosoma. Previous findings have suggested a relationship between infection with Schistosoma spp. and alterations in the liver and spleen of infected animals. Recent reports have shown the regulatory role of noncoding RNAs, such as long noncoding RNAs (lncRNAs), in different biological processes. However, little is known about the role of lncRNAs in the mouse liver and spleen during Schistosoma japonicum infection. Methods In this study, we identified and investigated lncRNAs using standard RNA sequencing (RNA-Seq). The biological functions of the altered expression of lncRNAs and their target genes were predicted using bioinformatics. Ten dysregulated lncRNAs were selected randomly and validated in reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR) experiments. Results Our study identified 29,845 and 33,788 lncRNAs from the liver and spleen, respectively, of which 212 were novel lncRNAs. We observed that 759 and 789 of the lncRNAs were differentially expressed in the respective organs. The RT-qPCR results correlated well with the sequencing data. In the liver, 657 differentially expressed lncRNAs were predicted to target 2548 protein-coding genes, whereas in the spleen 660 differentially expressed lncRNAs were predicted to target 2673 protein-coding genes. Moreover, functional annotation showed that the target genes of the differentially expressed lncRNAs were associated with cellular processes, metabolic processes, and binding, and were significantly enriched in metabolic pathways, the cell cycle, ubiquitin-mediated proteolysis, and pathways in cancer. Conclusions Our study showed that numerous lncRNAs were differentially expressed in S. japonicum-infected liver and spleen compared to control liver and spleen; this suggested that lncRNAs may be involved in pathogenesis in the liver and spleen during S. japonicum infection.

scholarly journals Analysis of expression profiles of long noncoding RNAs and mRNAs in brains of mice infected by rabies virus by RNA sequencing

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Pingsen Zhao ◽  
Sudong Liu ◽  
Zhixiong Zhong ◽  
Tianqi Jiang ◽  
Ruiqiang Weng ◽  
...  
Keyword(s):  
Rna Sequencing ◽  
Rabies Virus ◽  
Expression Profiles ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas
Sign in / Sign up

Export Citation Format

Share Document