scholarly journals Human Metapneumovirus Infection Induces Significant Changes in Small Noncoding RNA Expression in Airway Epithelial Cells

2014 ◽  
Vol 3 ◽  
pp. e163 ◽  
Author(s):  
Junfang Deng ◽  
Ryan N Ptashkin ◽  
Qingrong Wang ◽  
Guangliang Liu ◽  
Guanping Zhang ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Noncoding Rna ◽  
Airway Epithelial Cells ◽  
Human Metapneumovirus ◽  
Rna Expression ◽  
Airway Epithelial ◽  
Small Noncoding Rna

scholarly journals Role of retinoic acid inducible gene-I in human metapneumovirus-induced cellular signalling

2008 ◽  
Vol 89 (8) ◽  
pp. 1978-1986 ◽  
Author(s):  
S. Liao ◽  
X. Bao ◽  
T. Liu ◽  
S. Lai ◽  
K. Li ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Epithelial Cells ◽  
Gene Transcription ◽  
Airway Epithelial Cells ◽  
Human Metapneumovirus ◽  
Airway Epithelial ◽  
Cellular Signalling ◽  
Hmpv Infection ◽  
Inducible Gene

Human metapneumovirus (HMPV) is a recently discovered pathogen that causes a significant proportion of respiratory infections in young infants, the elderly and immunocompromised patients. Very little is known regarding the cellular signalling elicited by this virus in airway epithelial cells, the target of HMPV infection. In this study, we investigated the role of the RNA helicases retinoic acid inducible gene-I (RIG-I) and melanoma differentiation-associated gene-5 (MDA-5) as the main pattern recognition receptors (PRRs) involved in viral detection and subsequent expression of proinflammatory and antiviral genes. HMPV infection readily induced RIG-I and MDA-5 gene and protein expression in A549 cells, a type II-like alveolar epithelial cell line. Expression of dominant-negative (DN) RIG-I or downregulation of RIG-I gene expression using small interfering RNA (siRNA) significantly decreased HMPV-induced beta interferon (IFN-β), interleukin (IL)-8 and RANTES gene transcription, by inhibiting viral-induced activation of nuclear factor (NF)-κB and interferon regulatory factor (IRF), leading to enhanced viral replication. On the other hand, MDA-5 did not seem to play a significant role in HMPV-induced cellular responses. Mitochondrial antiviral signalling protein (MAVS), an adaptor protein linking both RIG-I and MDA-5 to downstream activation of IRF-3 and NF-κB, was also necessary for HMPV-induced cellular signalling. Expression of a DN MAVS significantly reduced IFN-β and chemokine gene transcription, by inhibiting NF-κB- and IRF-dependent gene transcription, in response to HMPV infection. Our results show that HMPV activates the RIG-I–MAVS signalling pathway in airway epithelial cells, leading to the expression of important proinflammatory and antiviral molecules involved in the innate immune response to viruses.

scholarly journals Human Metapneumovirus Impairs Apoptosis of Nasal Epithelial Cells in Asthma via HSP70

2016 ◽  
Vol 9 (1) ◽  
pp. 52-64 ◽  
Author(s):  
Engin Baturcam ◽  
Natale Snape ◽  
Tiong Han Yeo ◽  
Johanna Schagen ◽  
Emma Thomas ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Viral Infections ◽  
Airway Epithelial Cells ◽  
Human Metapneumovirus ◽  
Airway Epithelial ◽  
Caspase Activation ◽  
Nasal Epithelial Cells ◽  
Hmpv Infection ◽  
Syncytial Virus

Asthmatics are highly susceptible to respiratory viral infections, possibly due to impaired innate immunity. However, the exact mechanisms of susceptibility are likely to differ amongst viruses. Therefore, we infected primary nasal epithelial cells (NECs) from adults with mild-to-moderate asthma, with respiratory syncytial virus (RSV) or human metapneumovirus (hMPV) in vitro and investigated the antiviral response. NECs from these asthmatics supported elevated hMPV but not RSV infection, compared to non-asthmatic controls. This correlated with reduced apoptosis and reduced activation of caspase-9 and caspase-3/7 in response to hMPV, but not RSV. The expression of heat shock protein 70 (HSP70), a known inhibitor of caspase activation and subsequent apoptosis, was amplified in response to hMPV infection. Chemical inhibition of HSP70 function restored caspase activation and reduced hMPV infection in NECs from asthmatic subjects. There was no impairment in the production of IFN by NECs from asthmatics in response to either hMPV or RSV, demonstrating that increased infection of asthmatic airway cells by hMPV is IFN-independent. This study demonstrates, for the first time, a mechanism for elevated hMPV infection in airway epithelial cells from adult asthmatics and identifies HSP70 as a potential target for antiviral and asthma therapies.

scholarly journals Identification of human metapneumovirus-induced gene networks in airway epithelial cells by microarray analysis

Virology ◽  
2008 ◽  
Vol 374 (1) ◽  
pp. 114-127 ◽  
Author(s):  
X. Bao ◽  
M. Sinha ◽  
T. Liu ◽  
C. Hong ◽  
B.A. Luxon ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Microarray Analysis ◽  
Gene Networks ◽  
Airway Epithelial Cells ◽  
Human Metapneumovirus ◽  
Airway Epithelial

scholarly journals Human metapneumovirus infection of airway epithelial cells is associated with changes in core metabolic pathways

Virology ◽  
2019 ◽  
Vol 531 ◽  
pp. 183-191 ◽  
Author(s):  
Yanhua Zhao ◽  
Harendra Singh Chahar ◽  
Narayana Komaravelli ◽  
Anar Dossumekova ◽  
Antonella Casola
Keyword(s):  
Epithelial Cells ◽  
Metabolic Pathways ◽  
Airway Epithelial Cells ◽  
Human Metapneumovirus ◽  
Airway Epithelial

Pro-inflammatory mediators increase levels of the noncoding RNA GAS5 in airway smooth muscle and epithelial cells

2015 ◽  
Vol 93 (3) ◽  
pp. 203-206 ◽  
Author(s):  
Christine R. Keenan ◽  
Michael J. Schuliga ◽  
Alastair G. Stewart
Keyword(s):  
Smooth Muscle ◽  
Epithelial Cells ◽  
Airway Smooth Muscle ◽  
Inflammatory Mediators ◽  
Noncoding Rna ◽  
Cell Types ◽  
Airway Epithelial Cells ◽  
Airway Epithelial ◽  
Structural Cell ◽  
Glucocorticoid Sensitivity

The long noncoding RNA (lncRNA) GAS5 has been found to act as a decoy for the glucocorticoid receptor (GR), thus implicating GAS5 as a potential regulator of glucocorticoid sensitivity and resistance. Airway smooth muscle (ASM) cells and airway epithelial cells (AEC) play an important role in the pathogenesis and persistence of asthma and other chronic airways diseases. These airway structural cell types are also important cellular targets of the anti-inflammatory actions of glucocorticoids. In this study, we sought to examine the relevance of GAS5 to glucocorticoid sensitivity and resistance in ASM and AEC. We provide the first evidence that pro-inflammatory mediators up-regulate GAS5 levels in both airway epithelial and smooth muscle cells, and that decreasing GAS5 levels can enhance glucocorticoid action in AEC.

scholarly journals Human Metapneumovirus Glycoprotein G Disrupts Mitochondrial Signaling in Airway Epithelial Cells

PLoS ONE ◽  
2013 ◽  
Vol 8 (4) ◽  
pp. e62568 ◽  
Author(s):  
Xiaoyong Bao ◽  
Deepthi Kolli ◽  
Junping Ren ◽  
Tianshuang Liu ◽  
Roberto P. Garofalo ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Airway Epithelial Cells ◽  
Human Metapneumovirus ◽  
Airway Epithelial ◽  
Glycoprotein G ◽  
Mitochondrial Signaling

scholarly journals Attenuated lncRNA NKILA Enhances the Secretory Function of Airway Epithelial Cells Stimulated by Mycoplasma pneumoniae via NF-κB

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Fengxia Zhang ◽  
Jiamin Zhang ◽  
Feng Liu ◽  
Yao Zhou ◽  
Yun Guo ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Mycoplasma Pneumoniae ◽  
Noncoding Rna ◽  
Nuclear Translocation ◽  
A549 Cells ◽  
Airway Epithelial Cells ◽  
Regulatory Function ◽  
Airway Epithelial ◽  
Secretory Function ◽  
Tnf Α

The secretory function of airway epithelial cells is important in the pathogenesis of Mycoplasma pneumoniae pneumonia (MPP). To investigate the regulatory function of NKILA (nuclear factor-κB (NF-κB) interacting long noncoding RNA (lncRNA)) in MPP, we first detected NKILA as well as the concentration of interleukin 8 (IL-8) and tumor necrosis factor-α (TNF-α) in bronchoalveolar lavage fluid of children with MPP. Then, NKILA was knocked down in epithelial cells to investigate its effect on their secretory function. The results suggested that NKILA was downregulated in children with MPP, while IL-8 and TNF-α levels increased. Knockdown of NKILA in vitro promoted the inflammatory effects of Mycoplasma pneumoniae (MP) in epithelial A549 and BEAS-2B cells. Knockdown of NKILA promoted inhibitor of κBα (IκBα) phosphorylation and degradation, and NF-κB p65 nuclear translocation. Furthermore, RNA immunoprecipitation showed that NKILA could physically bind to IκBα in MP-treated A549 cells. Collectively, our data demonstrated that attenuation of NKILA enhances the effects of MP-stimulated secretory functions of epithelial cells via regulation of NF-κB signaling.

Differentiation of human pluripotent stem cells into airway epithelial cells

Pneumologie ◽  
2015 ◽  
Vol 69 (07) ◽  
Author(s):  
S Ulrich ◽  
S Weinreich ◽  
R Haller ◽  
S Menke ◽  
R Olmer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Epithelial Cells ◽  
Pluripotent Stem Cells ◽  
Airway Epithelial Cells ◽  
Human Pluripotent Stem Cells ◽  
Airway Epithelial
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