scholarly journals The feasibility of incorporating Vpx into lentiviral gene therapy vectors

2016 ◽  
Vol 3 ◽  
pp. 16066 ◽  
Author(s):  
Samantha A McAllery ◽  
Chantelle L Ahlenstiel ◽  
Kazuo Suzuki ◽  
Geoff P Symonds ◽  
Anthony D Kelleher ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Gene Therapy Vectors ◽  
Lentiviral Gene Therapy

scholarly journals The CXCR4-Tropic Human Immunodeficiency Virus Envelope Promotes More-Efficient Gene Delivery to Resting CD4+ T Cells than the Vesicular Stomatitis Virus Glycoprotein G Envelope

2009 ◽  
Vol 83 (16) ◽  
pp. 8153-8162 ◽  
Author(s):  
Luis M. Agosto ◽  
Jianqing J. Yu ◽  
Megan K. Liszewski ◽  
Clifford Baytop ◽  
Nikolay Korokhov ◽  
...  
Keyword(s):  
Gene Therapy ◽  
T Cells ◽  
Vesicular Stomatitis Virus ◽  
Resting Cells ◽  
Vesicular Stomatitis Virus Glycoprotein ◽  
Glycoprotein G ◽  
Immunodeficiency Virus ◽  
Gene Therapy Vectors ◽  
Vesicular Stomatitis ◽  
Lentiviral Gene Therapy

ABSTRACT Current gene transfer protocols for resting CD4+ T cells include an activation step to enhance transduction efficiency. This step is performed because it is thought that resting cells are resistant to transduction by lentiviral-based gene therapy vectors. However, activating resting cells prior to transduction alters their physiology, with foreseeable and unforeseeable negative consequences. Thus, it would be desirable to transduce resting CD4+ T cells without activation. We recently demonstrated, contrary to the prevailing belief, that wild-type human immunodeficiency virus (HIV) integrates into resting CD4+ T cells. Based on that finding, we investigated whether a commonly used, vesicular stomatitis virus glycoprotein G (VSV-G)-pseudotyped lentiviral gene therapy vector could also integrate into resting CD4+ T cells. To investigate this, we inoculated resting CD4+ T cells with lentiviral particles that were pseudotyped with VSV-G or CXCR4-tropic HIV Env and assayed binding, fusion, reverse transcription, and integration. We found that the VSV-G-pseudotyped lentiviral vector failed to fuse to resting CD4+ T cells while HIV Env-pseudotyped lentiviral vectors fused, reverse transcribed, and integrated in resting cells. Our findings suggest that HIV Env could be used effectively for the delivery of therapeutic genes to resting CD4+ T cells and suggest that fusion may be the critical step restricting transduction of resting CD4+ T cells by lentiviral gene therapy vectors.

Downstream processing of oncoretroviral and lentiviral gene therapy vectors

2006 ◽  
Vol 24 (3) ◽  
pp. 321-337 ◽  
Author(s):  
María de las Mercedes Segura ◽  
Amine Kamen ◽  
Alain Garnier
Keyword(s):  
Gene Therapy ◽  
Downstream Processing ◽  
Gene Therapy Vectors ◽  
Lentiviral Gene Therapy

scholarly journals Quantitation of Trace Levels of DNA Released from Disrupted Adeno-Associated Virus Gene Therapy Vectors

2021 ◽  
Author(s):  
Jared S. Bee ◽  
Kristin O'Berry ◽  
Yu (Zoe) Zhang ◽  
Megan Kuhn Phillippi ◽  
Akanksha Kaushal ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Adeno Associated Virus ◽  
Virus Gene ◽  
Gene Therapy Vectors

A novel purification strategy for retrovirus gene therapy vectors using heparin affinity chromatography

2005 ◽  
Vol 90 (4) ◽  
pp. 391-404 ◽  
Author(s):  
Mar�a de las Mercedes Segura ◽  
Amine Kamen ◽  
Pierre Trudel ◽  
Alain Garnier
Keyword(s):  
Gene Therapy ◽  
Affinity Chromatography ◽  
Gene Therapy Vectors ◽  
Heparin Affinity

scholarly journals Recognition of Virus Infection and Innate Host Responses to Viral Gene Therapy Vectors

2010 ◽  
Vol 18 (8) ◽  
pp. 1422-1429 ◽  
Author(s):  
Dmitry M Shayakhmetov ◽  
Nelson C Di Paolo ◽  
Karen L Mossman
Keyword(s):  
Gene Therapy ◽  
Virus Infection ◽  
Host Responses ◽  
Viral Gene ◽  
Viral Gene Therapy ◽  
Gene Therapy Vectors
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