Large-scale production of lentiviral vector in a closed system hollow fiber bioreactor

Cellular agriculture is defined as the production of agricultural products from cell cultures rather than from whole plants or animals. With growing interest in cellular agriculture as a means to address the public health, environmental, and animal welfare challenges of animal agriculture, the concept of producing seafood from fish cell- and tissue-cultures is emerging as a means to address similar challenges with industrial aquaculture systems and marine capture. Cell-based seafood - as opposed to animal-based seafood - can combine developments in biomedical engineering with modern aquaculture techniques. Biomedical engineering developments such as closed-system bioreactor production of land animal cells create a basis for large scale production of marine animal cells. Aquaculture techniques such as genetic modification and closed system aquaculture have achieved marked gains in production that can pave the way for innovations in cell-based seafood production. Here, we present the current state of innovation relevant to the development of cell-based seafood across multiple species as well as specific opportunities and challenges that exist for advancing this science. The authors find that the physiological properties of fish cell- and tissue- culture may be uniquely suited to cultivation in vitro. These physiological properties, including hypoxia tolerance, high buffering capacity, and low-temperature growth conditions, make marine cell culture an attractive opportunity for scale production of cell-based seafood; perhaps even more so than mammalian and avian cell cultures for cell-based meats. This, coupled with the unique capabilities of crustacean tissue-friendly scaffolding such as chitosan, a common seafood waste product and mushroom derivative, presents great promise for cell-based seafood production via bioreactor cultivation. To become fully realized, cell-based seafood research will require more understanding of fish muscle culture and cultivation; more investigation into serum-free media formulations optimized for fish cell culture; and bioreactor designs tuned to the needs of fish cells for large scale production.

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Large Scale Production of Vesicles by Hollow Fiber Extrusion: A Novel Method for Generating Polymersome Encapsulated Hemoglobin Dispersions

Langmuir ◽

10.1021/la9036343 ◽

2010 ◽

Vol 26 (7) ◽

pp. 5279-5285 ◽

Cited By ~ 26

Author(s):

Shahid Rameez ◽

Ibrahim Bamba ◽

Andre F. Palmer

Keyword(s):

Hollow Fiber ◽

Large Scale ◽

Scale Production ◽

Large Scale Production ◽

Novel Method

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A low-cost automated device incorporating a hollow fiber filtration cartridge for large-scale production of ghosts from human erythrocytes

Journal of Biochemical and Biophysical Methods ◽

10.1016/0165-022x(90)90005-w ◽

1990 ◽

Vol 21 (4) ◽

pp. 299-309 ◽

Cited By ~ 13

Author(s):

Soumaya El Ouggouti ◽

Alain Truskolaski ◽

Annette Bussel ◽

Dodier Dhermy ◽

Olivier Bertrand

Keyword(s):

Hollow Fiber ◽

Large Scale ◽

Low Cost ◽

Human Erythrocytes ◽

Scale Production ◽

Large Scale Production

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Evaluation of hollow fiber culture for large-scale production of mouse embryonic stem cell-derived hematopoietic stem cells

Cytotechnology ◽

10.1007/s10616-018-0210-z ◽

2018 ◽

Vol 70 (3) ◽

pp. 975-982 ◽

Cited By ~ 1

Author(s):

Yu Nakano ◽

Shinya Iwanaga ◽

Hiroshi Mizumoto ◽

Toshihisa Kajiwara

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Embryonic Stem Cell ◽

Hollow Fiber ◽

Large Scale ◽

Embryonic Stem ◽

Mouse Embryonic Stem Cell ◽

Scale Production ◽

Hematopoietic Stem ◽

Large Scale Production

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Generation of a packaging cell line for prolonged large-scale production of high-titer HIV-1-based lentiviral vector

The Journal of Gene Medicine ◽

10.1002/jgm.726 ◽

2005 ◽

Vol 7 (6) ◽

pp. 818-834 ◽

Cited By ~ 54

Author(s):

Yajin Ni ◽

Susan Sun ◽

Ibe Oparaocha ◽

Laurent Humeau ◽

Brian Davis ◽

...

Keyword(s):

Cell Line ◽

Large Scale ◽

Lentiviral Vector ◽

High Titer ◽

Scale Production ◽

Packaging Cell Line ◽

Packaging Cell ◽

Large Scale Production ◽

Hiv 1

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Efficient large-scale production and concentration of HIV-1-based lentiviral vectors for use in vivo

Physiological Genomics ◽

10.1152/physiolgenomics.00135.2002 ◽

2003 ◽

Vol 12 (3) ◽

pp. 221-228 ◽

Cited By ~ 120

Author(s):

Jason E. Coleman ◽

Matthew J. Huentelman ◽

Sergey Kasparov ◽

Beverly L. Metcalfe ◽

Julian F. R. Paton ◽

...

Keyword(s):

Animal Studies ◽

Large Scale ◽

Lentiviral Vector ◽

High Titer ◽

Lentiviral Vectors ◽

Efficient Production ◽

Scale Production ◽

Peripheral Tissues ◽

Large Scale Production

The aim of this study was to develop an efficient method for packaging and concentrating lentiviral vectors that consistently yields high-titer virus on a scale suitable for in vivo applications. Transient cotransfection of 293T packaging cells with DNA plasmids encoding lentiviral vector components was optimized using SuperFect, an activated dendrimer-based transfection reagent. The use of SuperFect allowed reproducible and efficient production of high-titer lentiviral vector at concentrations greater than 1 × 107transducing units per ml (TU/ml) and required less than one-third of the total amount of DNA used in traditional calcium phosphate transfection methods. Viral titers were further increased using a novel concentration protocol that yielded an average final titer of 1.4 × 1010TU/ml. Lentiviruses produced using these methods exhibited efficient transduction of central nervous system and peripheral tissues in vivo. The method is reproducible and can be scaled up to facilitate the use of these vectors in animal studies.

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