scholarly journals Hypoxia Preconditioned Mesenchymal Stem Cells Improve Vascular and Skeletal Muscle Fiber Regeneration After Ischemia Through a Wnt4-dependent Pathway

2010 ◽  
Vol 18 (8) ◽  
pp. 1545-1552 ◽  
Author(s):  
Lionel Leroux ◽  
Betty Descamps ◽  
Nancy F Tojais ◽  
Benjamin Séguy ◽  
Pierre Oses ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Muscle Fiber ◽  
Skeletal Muscle Fiber ◽  
Dependent Pathway ◽  
Muscle Fiber Regeneration

scholarly journals Histological aspects of skeletal muscle fibers splitting of C57BL/6NCrl mice

2020 ◽  
pp. 291-296 ◽  
Author(s):  
P. Makovický ◽  
P. Makovický
Keyword(s):  
Skeletal Muscle ◽  
Muscle Fiber ◽  
Skeletal Muscles ◽  
Muscle Fibers ◽  
Rectus Femoris ◽  
Skeletal Muscle Fiber ◽  
Fiber Splitting ◽  
Muscular Fiber ◽  
Skeletal Muscle Fibers ◽  
Muscle Fiber Regeneration

The objective of the current study is to present data on the splitting of skeletal muscle fibers in C57BL/6NCrl mice. Skeletal muscles (m. rectus femoris (m. quadriceps femoris)) from 500 (250 ♀ and 250 ♂) C57BL/6NCrl mice in the 16th week of life were sampled during autopsy and afterwards standardly histologically processed. Results show spontaneous skeletal muscle fiber splitting which is followed by skeletal muscle fiber regeneration. One solitary skeletal muscle fiber is split, or is in contact with few localized splitting skeletal muscle fibers. Part of the split skeletal muscular fiber is phagocytosed, but the remaining skeletal muscular fiber splits are merged into one regenerating skeletal muscle fiber. Nuclei move from the periphery to the regenerating skeletal muscle fiber center during this process. No differences were observed between female and male mice and the morphometry results document <1 % skeletal muscle fiber splitting. If skeletal muscular fibers splitting occurs 5 %> of all skeletal muscular fibers, it is suggested to describe and calculate this in the final histopathological report.

scholarly journals Skeletal muscle fiber regeneration following heterotopic autotransplantation in cats

Life Sciences ◽  
1976 ◽  
Vol 19 (2) ◽  
pp. 289-296 ◽  
Author(s):  
J.A. Faulkner ◽  
L.C. Maxwell ◽  
S.A. Mufti ◽  
B.M. Carlson
Keyword(s):  
Skeletal Muscle ◽  
Muscle Fiber ◽  
Skeletal Muscle Fiber ◽  
Muscle Fiber Regeneration

How to sample the entire length of a single muscle fiber quick-frozen after electrical point stimulation for high resolution EM

1992 ◽  
Vol 50 (1) ◽  
pp. 776-777
Author(s):  
Joachim R. Sommer ◽  
Teresa High ◽  
Betty Scherer ◽  
Isaiah Taylor ◽  
Rashid Nassar
Keyword(s):  
Skeletal Muscle ◽  
High Resolution ◽  
Action Potential ◽  
Muscle Fiber ◽  
Freeze Fracture ◽  
Freeze Substitution ◽  
Electrical Field Stimulation ◽  
Skeletal Muscle Fiber ◽  
Freeze Dried ◽  
Quick Freezing

We have developed a model that allows the quick-freezing at known time intervals following electrical field stimulation of a single, intact frog skeletal muscle fiber isolated by sharp dissection. The preparation is used for studying high resolution morphology by freeze-substitution and freeze-fracture and for electron probe x-ray microanlysis of sudden calcium displacement from intracellular stores in freeze-dried cryosections, all in the same fiber. We now show the feasibility and instrumentation of new methodology for stimulating a single, intact skeletal muscle fiber at a point resulting in the propagation of an action potential, followed by quick-freezing with sub-millisecond temporal resolution after electrical stimulation, followed by multiple sampling of the frozen muscle fiber for freeze-substitution, freeze-fracture (not shown) and cryosectionmg. This model, at once serving as its own control and obviating consideration of variances between different fibers, frogs etc., is useful to investigate structural and topochemical alterations occurring in the wake of an action potential.

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