scholarly journals Cell–cell interaction networks regulate blood stem and progenitor cell fate

2009 ◽  
Vol 5 (1) ◽  
pp. 293 ◽  
Author(s):  
Daniel C Kirouac ◽  
Gerard J Madlambayan ◽  
Mei Yu ◽  
Edward A Sykes ◽  
Caryn Ito ◽  
...  
Keyword(s):  
Cell Fate ◽  
Progenitor Cell ◽  
Cell Interaction ◽  
Interaction Networks ◽  
Cell Cell

scholarly journals On the role of lateral stabilization during early patterning in the pancreas

2013 ◽  
Vol 10 (79) ◽  
pp. 20120766 ◽  
Author(s):  
Walter de Back ◽  
Joseph Xu Zhou ◽  
Lutz Brusch
Keyword(s):  
Progenitor Cells ◽  
Cell Fate ◽  
Lateral Inhibition ◽  
Endocrine Cells ◽  
Cell Interaction ◽  
Simultaneous Occurrence ◽  
Cell Type ◽  
Pancreatic Progenitor ◽  
Cell Cell

The cell fate decision of multi-potent pancreatic progenitor cells between the exocrine and endocrine lineages is regulated by Notch signalling, mediated by cell–cell interactions. However, canonical models of Notch-mediated lateral inhibition cannot explain the scattered spatial distribution of endocrine cells and the cell-type ratio in the developing pancreas. Based on evidence from acinar-to-islet cell transdifferentiation in vitro , we propose that lateral stabilization, i.e. positive feedback between adjacent progenitor cells, acts in parallel with lateral inhibition to regulate pattern formation in the pancreas. A simple mathematical model of transcriptional regulation and cell–cell interaction reveals the existence of multi-stability of spatial patterns whose simultaneous occurrence causes scattering of endocrine cells in the presence of noise. The scattering pattern allows for control of the endocrine-to-exocrine cell-type ratio by modulation of lateral stabilization strength. These theoretical results suggest a previously unrecognized role for lateral stabilization in lineage specification, spatial patterning and cell-type ratio control in organ development.

Specification of ectodermal teloblast lineages in embryos of the oligochaete annelid Tubifex: involvement of novel cell-cell interactions

Development ◽  
2001 ◽  
Vol 128 (7) ◽  
pp. 1211-1219 ◽  
Author(s):  
A. Arai ◽  
A. Nakamoto ◽  
T. Shimizu
Keyword(s):  
Cell Interaction ◽  
Cell Interactions ◽  
Interaction Networks ◽  
Germ Band ◽  
Morphological Pattern ◽  
Cell Divisions ◽  
Cell Ablation ◽  
Oligochaete Annelid ◽  
Inductive Signal ◽  
Cell Cell

In embryos of clitellate annelids (i.e. oligochaetes and leeches), four ectodermal teloblasts (ectoteloblasts N, O, P and Q) are generated on either side through a stereotyped sequence of cell divisions of a proteloblast, NOPQ. The four ectoteloblasts assume distinct fates and produce bandlets of smaller progeny cells, which join together to form an ectodermal germ band. The pattern of the germ band, with respect to the ventrodorsal order of the bandlets, has been highly preserved in clitellate annelids. We show that specification of ectoteloblast lineages in the oligochaete annelid Tubifex involves cell interaction networks distinct from those in leeches. Cell ablation experiments have shown that fates of teloblasts N, P and Q in Tubifex embryos are determined rigidly as early as their birth. In contrast, the O teloblast and its progeny are initially pluripotent and their fate becomes restricted to the O fate through an inductive signal emanating from the P lineage. In the absence of this signal, the O lineage assumes the P fate. These results differ significantly from those obtained in embryos of the leech Helobdella, suggesting the diversity of patterning mechanisms that give rise to germ bands with similar morphological pattern.

scholarly journals New Consensus pattern in Spike CoV-2: potential implications in coagulation process and cell–cell fusion

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Silvia Buonvino ◽  
Sonia Melino
Keyword(s):  
Cell Fate ◽  
Cell Fusion ◽  
Molecular Mechanisms ◽  
Coagulation Factor ◽  
Cell Interaction ◽  
Factor X ◽  
S Protein ◽  
Coagulation Process ◽  
Consensus Pattern ◽  
Cell Cell

AbstractCoagulopathy and syncytial formation are relevant effects of the SARS-CoV-2 infection, but the underlying molecular mechanisms triggering these processes are not fully elucidated. Here, we identified a potential consensus pattern in the Spike S glycoprotein present within the cytoplasmic domain; this consensus pattern was detected in only 79 out of 561,000 proteins (UniProt bank). Interestingly, the pattern was present in both human and bat the coronaviruses S proteins, in many proteins involved in coagulation process, cell–cell interaction, protein aggregation and regulation of cell fate, such as von Willebrand factor, coagulation factor X, fibronectin and Notch, characterized by the presence of the cysteine-rich EGF-like domain. This finding may suggest functional similarities between the matched proteins and the CoV-2 S protein, implying a new possible involvement of the S protein in the molecular mechanism that leads to the coagulopathy and cell fusion in COVID-19 disease.

scholarly journals Mouse notch: expression in hair follicles correlates with cell fate determination.

1993 ◽  
Vol 121 (3) ◽  
pp. 631-641 ◽  
Author(s):  
R Kopan ◽  
H Weintraub
Keyword(s):  
Hair Follicle ◽  
Cell Fate ◽  
Dermal Papilla ◽  
Cell Types ◽  
Cell Interaction ◽  
Hair Follicles ◽  
Skin Development ◽  
High Level ◽  
Cell Cell

Many vertebrate tissues, including skin, are known to develop as a consequence of epithelial-mesenchymal interactions. Much less is known about the role of cell-cell interaction within the epithelial or the mesenchymal compartments in morphogenesis. To investigate cell-cell interactions during skin development, and the potential role of the Notch homolog in this process, we cloned the mouse homolog of Notch (mNotch) and studied its expression pattern, starting as early as mesoderm formation. The novel application of double-labeled in situ hybridization in vertebrates allowed high resolution analysis to follow the fate of mNotch expressing cells directly. In comparison with the distribution of Id mRNA, analysis confirmed that in the hair follicle high levels of mNotch are expressed exclusively in the epithelial compartment. Hair follicle matrix cells start expressing mNotch as different cell types become distinguishable in the developing follicle. mNotch mRNA expression persists throughout the growth phase of the follicle and maintains the same expression profile in the second hair cycle. The cells in the follicle that undergo a phase of high level mNotch expression are in transition from mitotic precursors to several discreet, differentiating cell types. Our observations point out that both in time (during development) and in space (by being removed one cell layer from the dermal papilla) mNotch expression is clearly separated from the inductive interactions. This is a novel finding and suggests that mNotch is important for follicular differentiation and possibly cell fate selection within the follicle.

scholarly journals Modulation of Cell–Cell Interactions in Drosophila Oocyte Development

Cells ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 274
Author(s):  
Matthew Antel ◽  
Mayu Inaba
Keyword(s):  
Cell Communication ◽  
Cell Interaction ◽  
Oocyte Development ◽  
Suitable Model ◽  
Physiological Regulation ◽  
Temporal Regulation ◽  
Cellular Processes ◽  
Cellular Machinery ◽  
Drosophila Ovary ◽  
Cell Cell

The Drosophila ovary offers a suitable model system to study the mechanisms that orchestrate diverse cellular processes. Oogenesis starts from asymmetric stem cell division, proper differentiation and the production of fully patterned oocytes equipped with all the maternal information required for embryogenesis. Spatial and temporal regulation of cell-cell interaction is particularly important to fulfill accurate biological outcomes at each step of oocyte development. Progress has been made in understanding diverse cell physiological regulation of signaling. Here we review the roles of specialized cellular machinery in cell-cell communication in different stages of oogenesis.

Interferon β-1b directly modulates human neural stem/progenitor cell fate

2011 ◽  
Vol 1413 ◽  
pp. 1-8 ◽  
Author(s):  
W. Tristram Arscott ◽  
John Soltys ◽  
Julia Knight ◽  
Yang Mao-Draayer
Keyword(s):  
Cell Fate ◽  
Progenitor Cell ◽  
Interferon Β

scholarly journals SUMOylation Negatively Regulates Angiogenesis by Targeting Endothelial NOTCH Signaling

2017 ◽  
Vol 121 (6) ◽  
pp. 636-649 ◽  
Author(s):  
Xiaolong Zhu ◽  
Sha Ding ◽  
Cong Qiu ◽  
Yanna Shi ◽  
Lin Song ◽  
...  
Keyword(s):  
Notch Signaling ◽  
Cell Fate ◽  
Developmental Disorders ◽  
Interaction Mechanism ◽  
Growth Factor Receptor ◽  
Vascular Diseases ◽  
Cell Interaction ◽  
Protein Signaling

Rationale: The highly conserved NOTCH (neurogenic locus notch homolog protein) signaling pathway functions as a key cell–cell interaction mechanism controlling cell fate and tissue patterning, whereas its dysregulation is implicated in a variety of developmental disorders and cancers. The pivotal role of endothelial NOTCH in regulation of angiogenesis is widely appreciated; however, little is known about what controls its signal transduction. Our previous study indicated the potential role of post-translational SUMO (small ubiquitin-like modifier) modification (SUMOylation) in vascular disorders. Objective: The aim of this study was to investigate the role of SUMOylation in endothelial NOTCH signaling and angiogenesis. Methods and Results: Endothelial SENP1 (sentrin-specific protease 1) deletion, in newly generated endothelial SENP1 (the major protease of the SUMO system)–deficient mice, significantly delayed retinal vascularization by maintaining prolonged NOTCH1 signaling, as confirmed in cultured endothelial cells. An in vitro SUMOylation assay and immunoprecipitation revealed that when SENP1 associated with N1ICD (NOTCH1 intracellular domain), it functions as a deSUMOylase of N1ICD SUMOylation on conserved lysines. Immunoblot and immunoprecipitation analyses and dual-luciferase assays of natural and SUMO-conjugated/nonconjugated NOTCH1 forms demonstrated that SUMO conjugation facilitated NOTCH1 cleavage. This released N1ICD from the membrane and stabilized it for translocation to the nucleus where it functions as a cotranscriptional factor. Functionally, SENP1-mediated NOTCH1 deSUMOylation was required for NOTCH signal activation in response to DLL4 (Delta-like 4) stimulation. This in turn suppressed VEGF (vascular endothelial growth factor) receptor signaling and angiogenesis, as evidenced by immunoblotted signaling molecules and in vitro angiogenesis assays. Conclusions: These results establish reversible NOTCH1 SUMOylation as a regulatory mechanism in coordinating endothelial angiogenic signaling; SENP1 acts as a critical intrinsic mediator of this process. These findings may apply to NOTCH-regulated biological events in nonvascular tissues and provide a novel therapeutic strategy for vascular diseases and tumors.

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