scholarly journals Small hairpin RNA as a small molecule sensor

2008 ◽  
Vol 4 (1) ◽  
pp. 227 ◽  
Author(s):  
Yaakov Benenson
Keyword(s):  
Small Molecule ◽  
Hairpin Rna ◽  
Small Hairpin Rna

scholarly journals Knockdown of Glutamate-Cysteine Ligase by Small Hairpin RNA Reveals That Both Catalytic and Modulatory Subunits Are Essential for the Survival of Primary Neurons

2005 ◽  
Vol 280 (47) ◽  
pp. 38992-39001 ◽  
Author(s):  
Juan I. Diaz-Hernandez ◽  
Angeles Almeida ◽  
Maria Delgado-Esteban ◽  
Emilio Fernandez ◽  
Juan P. Bolaños
Keyword(s):  
Primary Neurons ◽  
Hairpin Rna ◽  
Glutamate Cysteine Ligase ◽  
Small Hairpin Rna

Silencing of Bc1-2 gene by small hairpin RNA inhibits growth of NB4 cells

2006 ◽  
Vol 18 (4) ◽  
pp. 257-260
Author(s):  
Dong-mei He ◽  
Yuan Zhang ◽  
Ge-xiu Liu
Keyword(s):  
Hairpin Rna ◽  
Nb4 Cells ◽  
Small Hairpin Rna

scholarly journals 5-HT1A receptors of the nucleus tractus solitarii facilitate sympathetic recovery following hypotensive hemorrhage in rats

2015 ◽  
Vol 309 (2) ◽  
pp. H335-H344 ◽  
Author(s):  
Jaime E. Vantrease ◽  
Nichole Dudek ◽  
Lydia L. DonCarlos ◽  
Karie E. Scrogin
Keyword(s):  
Blood Loss ◽  
Sympathetic Nerve ◽  
Sympathetic Nerve Activity ◽  
Tissue Perfusion ◽  
Nucleus Tractus Solitarii ◽  
Nerve Activity ◽  
Hairpin Rna ◽  
Renal Sympathetic Nerve Activity ◽  
Small Hairpin Rna ◽  
Renal Sympathetic

The role of serotonin in the hemodynamic response to blood loss remains controversial. Caudal raphe serotonin neurons are activated during hypotensive hemorrhage, and their destruction attenuates sympathetic increases following blood loss in unanesthetized rats. Caudal raphe neurons provide serotonin-positive projections to the nucleus tractus solitarii (NTS), and disruption of serotonin-positive nerve terminals in the NTS attenuates sympathetic recovery following hemorrhage. Administration of 5-HT1A-receptor agonists following hemorrhage augments sympathetic-mediated increases in venous tone and tissue hypoxia. These findings led us to hypothesize that severe blood loss promotes activation of 5-HT1A receptors in the NTS, which facilitates sympathetic recovery and peripheral tissue perfusion. Here, we developed an adeno-associated viral vector encoding an efficacious small hairpin RNA sequence targeting the rat 5-HT1A receptor. Unanesthetized rats subjected to NTS injection of the anti-rat 5-HT1A small hairpin RNA-encoding vector 4 wk prior showed normal blood pressure recovery, but an attenuated recovery of renal sympathetic nerve activity (−6.4 ± 12.9 vs. 42.6 ± 15.6% baseline, P < 0.05) 50 min after 21% estimated blood volume withdrawal. The same rats developed increased tissue hypoxia after hemorrhage, as indicated by prolonged elevations in lactate (2.77 ± 0.5 vs. 1.34 ± 0.2 mmol/l, 60 min after start of hemorrhage, P < 0.05). 5-HT1A mRNA levels in the commissural NTS were directly correlated with renal sympathetic nerve activity ( P < 0.01) and inversely correlated with lactate ( P < 0.05) 60 min after start of hemorrhage. The data suggest that 5-HT1A receptors in the commissural NTS facilitate tissue perfusion after blood loss likely by increasing sympathetic-mediated venous return.

Suppressing the growth of rectal cancer xenografts derived from patient tumors by an adenovector expressing small hairpin RNA targeting Bcl-XL

2012 ◽  
Vol 14 (12) ◽  
pp. 761-768 ◽  
Author(s):  
Hongbo Zhu ◽  
Wei Zhou ◽  
Jingzi Hu ◽  
Zhongting Huang ◽  
Weifeng Lao ◽  
...  
Keyword(s):  
Rectal Cancer ◽  
Hairpin Rna ◽  
Small Hairpin Rna ◽  
Rna Targeting
Sign in / Sign up

Export Citation Format

Share Document