scholarly journals Laser Capture Microdissection–Guided Fluorescence In Situ Hybridization and Flow Cytometric Cell Cycle Analysis of Purified Nuclei from Paraffin Sections

2000 ◽  
Vol 13 (6) ◽  
pp. 705-711 ◽  
Author(s):  
Lisa M DiFrancesco ◽  
Sabita K Murthy ◽  
Joanne Luider ◽  
Douglas J Demetrick
Keyword(s):  
Cell Cycle ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Laser Capture Microdissection ◽  
Cell Cycle Analysis ◽  
Paraffin Sections ◽  
Flow Cytometric ◽  
Laser Capture ◽  
Flow Cytometric Cell

Localization of Aa-nat mRNA in the rat retina by fluorescence in situ hybridization and laser capture microdissection

2003 ◽  
Vol 315 (2) ◽  
pp. 197-201 ◽  
Author(s):  
Cuimei Liu ◽  
Chiaki Fukuhara ◽  
James H. Wessel ◽  
P. Michael Iuvone ◽  
Gianluca Tosini
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Laser Capture Microdissection ◽  
Rat Retina ◽  
Laser Capture

scholarly journals Laser capture microdissection of bacterial cells targeted by fluorescence in situ hybridization

BioTechniques ◽  
2005 ◽  
Vol 39 (6) ◽  
pp. 864-868 ◽  
Author(s):  
Kirstine Klitgaard ◽  
Lars Mølbak ◽  
Tim K. Jensen ◽  
Christian Fredrik Lindboe ◽  
Mette Boye
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Laser Capture Microdissection ◽  
Bacterial Cells ◽  
Laser Capture

Multi-parametric flow cytometric cell cycle analysis using TO-PRO-3 iodide (TP3): Detailed protocols

2008 ◽  
Vol 110 (3) ◽  
pp. 232-244 ◽  
Author(s):  
Michele Tavecchio ◽  
Matteo Simone ◽  
Sergio Bernasconi ◽  
Gianluca Tognon ◽  
Giuliano Mazzini ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Analysis ◽  
Flow Cytometric ◽  
Flow Cytometric Cell

HER-2/neu Analysis in Archival Tissue Samples of Human Breast Cancer: Comparison of Immunohistochemistry and Fluorescence In Situ Hybridization

2001 ◽  
Vol 19 (2) ◽  
pp. 354-363 ◽  
Author(s):  
Annette Lebeau ◽  
Daniela Deimling ◽  
Christine Kaltz ◽  
Andrea Sendelhofert ◽  
Anette Iff ◽  
...  
Keyword(s):  
Breast Cancer ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Gene Amplification ◽  
Cancer Tissue ◽  
Breast Cancers ◽  
Paraffin Sections ◽  
Tissue Samples ◽  
Her 2

PURPOSE: The objective of our study was to compare the methods used in the literature to analyze HER-2/neu status on archival breast cancer tissue. Therefore, a series of antibodies was evaluated to assess their immunohistochemical (IHC) sensitivity in correlation to gene amplification determined by fluorescence in situ hybridization (FISH). MATERIALS AND METHODS: HER-2/neu overexpression was studied on paraffin sections of 85 invasive breast cancers using a panel of five monoclonal (9G6, 3B5, CB11, TAB250, GSF-HER2) and two polyclonal antibodies (A8010, A0485) in addition to the HercepTest (DAKO, Glostrup, Denmark). HER-2/neu gene amplification was determined by FISH using a dual-color probe (PathVysion; Vysis, Stuttgart-Fasanenhof, Germany). RESULTS: HER-2/neu overexpression was demonstrated in 26% (9G6, TAB250, GSF-HER2), 27% (3B5, CB11), 33% (A8010) and 42% (A0485, HercepTest) of the tumors. FISH on paraffin sections identified gene amplification in 28% of the tumors. Strongly positive IHC results (3+) were always associated with gene amplification. Among the 16 tumors presented with weakly positive IHC results (2+) using the HercepTest, 12 (75%) lacked gene amplification. CONCLUSION: The comparison of IHC and FISH demonstrated an excellent correlation of high-level HER-2/neu overexpression (3+) with gene amplification; ie, FISH does not provide further information in these tumors. However, weakly positive IHC results (2+) obtained with the HercepTest share only a minor association with gene amplification.

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