scholarly journals Thyroid Transcription Factor-1 in Normal, Hyperplastic, and Neoplastic Follicular Thyroid Cells Examined by Immunohistochemistry and Nonradioactive In Situ Hybridization

2000 ◽  
Vol 13 (5) ◽  
pp. 570-576 ◽  
Author(s):  
Ryohei Katoh ◽  
Akira Kawaoi ◽  
Eri Miyagi ◽  
Xin Li ◽  
Koichi Suzuki ◽  
...  
Keyword(s):  
Transcription Factor ◽  
In Situ Hybridization ◽  
Thyroid Cells ◽  
Thyroid Transcription Factor 1 ◽  
Thyroid Transcription Factor ◽  
Transcription Factor 1 ◽  
Nonradioactive In Situ Hybridization

Role of Insulin and Serum on Thyrotropin Regulation of Thyroid Transcription Factor-1 and Pax-8 Genes Expression in FRTL-5 Thyroid Cells

Thyroid ◽  
2000 ◽  
Vol 10 (4) ◽  
pp. 295-303 ◽  
Author(s):  
Diego L. Medina ◽  
Koichi Suzuki ◽  
Michele Pietrarelli ◽  
Fumi Okajima ◽  
Leonard D. Kohn ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Thyroid Cells ◽  
Thyroid Transcription Factor 1 ◽  
Thyroid Transcription Factor ◽  
Genes Expression ◽  
Transcription Factor 1

Functional inactivation of thyroid transcription factor-1 in PCCl3 thyroid cells

2012 ◽  
Vol 358 (1) ◽  
pp. 36-45 ◽  
Author(s):  
Christiane Christophe-Hobertus ◽  
Anne Lefort ◽  
Frederick Libert ◽  
Daniel Christophe
Keyword(s):  
Transcription Factor ◽  
Thyroid Cells ◽  
Thyroid Transcription Factor 1 ◽  
Thyroid Transcription Factor ◽  
Functional Inactivation ◽  
Transcription Factor 1

scholarly journals Thyroid Transcription Factor 1 Rescues PAX8/p300 Synergism Impaired by a Natural PAX8 Paired Domain Mutation with Dominant Negative Activity

2005 ◽  
Vol 19 (7) ◽  
pp. 1779-1791 ◽  
Author(s):  
Helmut Grasberger ◽  
Usanee Ringkananont ◽  
Paule LeFrancois ◽  
Marc Abramowicz ◽  
Gilbert Vassart ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Dna Binding ◽  
Dominant Negative ◽  
Dominant Negative Effect ◽  
Wild Type ◽  
Paired Domain ◽  
Thyroid Cells ◽  
Thyroid Transcription Factor 1 ◽  
Thyroid Transcription Factor ◽  
Transcription Factor 1

Abstract Mutations in the paired domain transcription factor PAX8 are a rare cause of congenital hypothyroidism due to thyroid dysgenesis. We identified a novel and unique PAX8 mutation segregating in seven affected members of a three-generations family. The mutation replaces an invariant serine residue within helix 2 of the paired DNA-binding domain for phenylalanine. The mutant protein (PAX8-S48F) does not induce the thyroglobulin promoter in nonthyroid cells, but displays almost half of wild-type PAX8 activity in thyroid cells. PAX8-S48F shows no defect in expression, nuclear targeting, or DNA binding and retains the ability to synergize with thyroid transcription factor 1 (TTF-1, NKX2.1). However, we found that in nonthyroid cells, the acetylation-independent synergism with the general transcriptional adaptor p300 is completely abrogated, suggesting that PAX8-S48F may be unable to efficiently recruit p300. Reconstitution experiments in nonthyroid cells reveal that TTF-1 can partially rescue PAX8-S48F/p300 synergism and thus reproduce the situation in thyroid cells. These functional characteristics result in a dominant negative effect of PAX8-S48F on coexpressed wild-type PAX8 activity, which is not observed in paired domain mutations with DNA binding defect. Our results describe the first dominant negative missense mutation in a paired domain and provide evidence for a crucial role of the p300 coactivator in mediating the functional synergism between PAX8 and TTF-1 in thyroid-specific gene expression.

scholarly journals Identification of Thyroid Transcription Factor-1 in C Cells and Parathyroid Cells

Endocrinology ◽  
1998 ◽  
Vol 139 (6) ◽  
pp. 3014-3017 ◽  
Author(s):  
Koichi Suzuki ◽  
Yoshihiko Kobayashi ◽  
Ryohei Katoh ◽  
Leonard D. Kohn ◽  
Akira Kawaoi
Keyword(s):  
Transcription Factor ◽  
C Cells ◽  
Thyroid Transcription Factor 1 ◽  
Thyroid Transcription Factor ◽  
Transcription Factor 1

Thyroid transcription factor-1 expression in colorectal adenocarcinomas

2011 ◽  
Vol 207 (11) ◽  
pp. 686-690 ◽  
Author(s):  
Matthias Dettmer ◽  
Tae Eun Kim ◽  
Chan Kwon Jung ◽  
Eun Sun Jung ◽  
Kyo Young Lee ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Thyroid Transcription Factor 1 ◽  
Thyroid Transcription Factor ◽  
Transcription Factor 1 ◽  
Colorectal Adenocarcinomas
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