scholarly journals Protein overexpression and gene amplification of HER-2 and EGFR in colorectal cancers: an immunohistochemical and fluorescent in situ hybridization study

2004 ◽  
Vol 17 (8) ◽  
pp. 895-904 ◽  
Author(s):  
Akishi Ooi ◽  
Takuo Takehana ◽  
Xiaoling Li ◽  
Shioto Suzuki ◽  
Kazuyoshi Kunitomo ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Gene Amplification ◽  
Fluorescent In Situ Hybridization ◽  
Colorectal Cancers ◽  
Hybridization Study ◽  
Protein Overexpression ◽  
Her 2

scholarly journals Low Level ofHer-2Locus Amplification by Fluorescent In Situ Hybridization Does Not Correlate with Her-2 Protein Overexpression by Immunohistochemistry in Barrett's Esophagus

2010 ◽  
Vol 2010 ◽  
pp. 1-8 ◽  
Author(s):  
Agnieszka M. Rygiel ◽  
Francesca Milano ◽  
Fiebo J. ten Kate ◽  
Jacques J. G. H. M. Bergman ◽  
Kausillia K. Krishnadath
Keyword(s):  
In Situ Hybridization ◽  
Barrett’S Esophagus ◽  
Barrett's Esophagus ◽  
Fluorescent In Situ Hybridization ◽  
Chromosome 17 ◽  
Protein Overexpression ◽  
Therapeutic Implications ◽  
Low Level ◽  
Her 2

An accurate evaluation of theHer-2status has important prognostic and therapeutic implications in many carcinomas. The aim of the study was to correlateHer-2locus (17q11.2) amplification and chromosome 17 gains as assessed by fluorescent in situ hybridization (FISH) with Her-2 protein overexpression by immunohistochemistry (IHC) in patients with Barrett's esophagus (BE) and esophageal adenocarcinoma (EAC). We analyzed 34 patients withHer-2amplification and/or chromosome 17gains using FISH on brush cytology specimens. Seven patients (21%) showed highHer-2locus amplification (Her-2: Cep17≥5 : 1), 5 (15%) showed lowHer-2locus amplification (Her-2: Cep17≥2<5 : 1), and 22 (65%) displayed gains of chromosome 17 only. Further, we confirmedHer-2amplification on corresponding biopsies that were taken at the same occasion as the cytologybrushings. Then, we compared the FISH results with IHC data obtained from the corresponding biopsies and showed that low level of Her-2 amplification does not correlate with Her-2 protein overexpression (score+3/+2;P=1), in contrast to the high amplification level (P=.001). Thus, in our population of BE and EAC patients, low level ofHer-2amplification does not result in detectable level of Her-2 protein as assessed by IHC.

Fluorescent in situ Hybridization Detection of HER-2/neu Gene Amplification in Rhabdomyosarcoma

Pathobiology ◽  
1998 ◽  
Vol 66 (2) ◽  
pp. 59-63 ◽  
Author(s):  
Hon Fong L. Mark ◽  
Stephen Brown ◽  
Ci-Lin Sun ◽  
Mangala Samy ◽  
Alaa Afify
Keyword(s):  
In Situ Hybridization ◽  
Gene Amplification ◽  
Fluorescent In Situ Hybridization ◽  
Her 2 ◽  
Hybridization Detection

scholarly journals HER-2/neu gene amplification in stages I-IV breast cancer detected by fluorescent in situ hybridization

1999 ◽  
Vol 1 (3) ◽  
pp. 98-103 ◽  
Author(s):  
Hon Fong L Mark ◽  
Bassam Aswad ◽  
Nader Bassily ◽  
William Taylor ◽  
Stephen Brown ◽  
...  
Keyword(s):  
Breast Cancer ◽  
In Situ Hybridization ◽  
Gene Amplification ◽  
Fluorescent In Situ Hybridization ◽  
Her 2
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