scholarly journals Lipid droplet-associated PAT-proteins show frequent and differential expression in neoplastic steatogenesis

2010 ◽  
Vol 23 (3) ◽  
pp. 480-492 ◽  
Author(s):  
Beate K Straub ◽  
Esther Herpel ◽  
Stephan Singer ◽  
Ralf Zimbelmann ◽  
Kai Breuhahn ◽  
...  
Keyword(s):  
Differential Expression ◽  
Lipid Droplet ◽  
Pat Proteins

Lipid droplet-associated PAT-proteins show frequent and differential expression in neoplastic steatogenesis

2010 ◽  
Vol 48 (01) ◽  
Author(s):  
BK Straub ◽  
E Herpel ◽  
S Singer ◽  
LM Pawella ◽  
R Zimbelman ◽  
...  
Keyword(s):  
Differential Expression ◽  
Lipid Droplet ◽  
Pat Proteins

scholarly journals Effects of EPA and DHA on lipid droplet accumulation and mRNA abundance of PAT proteins in caprine monocytes

2013 ◽  
Vol 94 (2) ◽  
pp. 246-251 ◽  
Author(s):  
Cristina Lecchi ◽  
Guido Invernizzi ◽  
Alessandro Agazzi ◽  
Silvia Modina ◽  
Paola Sartorelli ◽  
...  
Keyword(s):  
Lipid Droplet ◽  
Mrna Abundance ◽  
Epa And Dha ◽  
Pat Proteins

scholarly journals New Method for Quantitation of Lipid Droplet Volume From Light Microscopic Images With an Application to Determination of PAT Protein Density on the Droplet Surface

2018 ◽  
Vol 66 (6) ◽  
pp. 447-465 ◽  
Author(s):  
Selma Y. Dejgaard ◽  
John F. Presley
Keyword(s):  
Lipid Droplet ◽  
Fluorescent Protein ◽  
Automated Analysis ◽  
Droplet Surface ◽  
New Method ◽  
Droplet Volume ◽  
Major Function ◽  
Pat Proteins ◽  
Protein Density

Determination of lipid droplet (LD) volume has depended on direct measurement of the diameter of individual LDs, which is not possible when LDs are small or closely apposed. To overcome this problem, we describe a new method in which a volume–fluorescence relationship is determined from automated analysis of calibration samples containing well-resolved LDs. This relationship is then used to estimate total cellular droplet volume in experimental samples, where the LDs need not be individually resolved, or to determine the volumes of individual LDs. We describe quantitatively the effects of various factors, including image noise, LD crowding, and variation in LD composition on the accuracy of this method. We then demonstrate this method by utilizing it to address a scientifically interesting question, to determine the density of green fluorescent protein (GFP)–tagged Perilipin-Adipocyte-Tail (PAT) proteins on the LD surface. We find that PAT proteins cover only a minority of the LD surface, consistent with models in which they primarily serve as scaffolds for binding of regulatory proteins and enzymes, but inconsistent with models in which their major function is to sterically block access to the droplet surface.

Differential expression of ASS1 in serous and non-serous ovarian carcinomas

2014 ◽  
pp. n/a-n/a ◽  
Author(s):  
Dong-Joo Cheon ◽  
Ann E. Walts ◽  
Jessica A. Beach ◽  
Jenny Lester ◽  
John S. Bomalaski ◽  
...  
Keyword(s):  
Differential Expression ◽  
Ovarian Carcinomas
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