scholarly journals Particle focusing by 3D inertial microfluidics

2017 ◽  
Vol 3 (1) ◽  
Author(s):  
Petra Paiè ◽  
Francesca Bragheri ◽  
Dino Di Carlo ◽  
Roberto Osellame
Keyword(s):  
Inertial Microfluidics ◽  
Particle Focusing

scholarly journals High throughput viscoelastic particle focusing and separation in spiral microchannels

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tharagan Kumar ◽  
Harisha Ramachandraiah ◽  
Sharath Narayana Iyengar ◽  
Indradumna Banerjee ◽  
Gustaf Mårtensson ◽  
...  
Keyword(s):  
High Resolution ◽  
High Throughput ◽  
Separation Efficiency ◽  
Point Of Care ◽  
Outer Wall ◽  
Reynolds Numbers ◽  
Inertial Microfluidics ◽  
Flow Rates ◽  
Particle Focusing ◽  
Sample Flow Rate

AbstractPassive particle manipulation using inertial and elasto-inertial microfluidics have received substantial interest in recent years and have found various applications in high throughput particle sorting and separation. For separation applications, elasto-inertial microfluidics has thus far been applied at substantial lower flow rates as compared to inertial microfluidics. In this work, we explore viscoelastic particle focusing and separation in spiral channels at two orders of magnitude higher Reynolds numbers than previously reported. We show that the balance between dominant inertial lift force, dean drag force and elastic force enables stable 3D particle focusing at dynamically high Reynolds numbers. Using a two-turn spiral, we show that particles, initially pinched towards the inner wall using an elasticity enhancer, PEO (polyethylene oxide), as sheath migrate towards the outer wall strictly based on size and can be effectively separated with high precision. As a proof of principle for high resolution particle separation, 15 µm particles were effectively separated from 10 µm particles. A separation efficiency of 98% for the 10 µm and 97% for the 15 µm particles was achieved. Furthermore, we demonstrate sheath-less, high throughput, separation using a novel integrated two-spiral device and achieved a separation efficiency of 89% for the 10 µm and 99% for the 15 µm particles at a sample flow rate of 1 mL/min—a throughput previously only reported for inertial microfluidics. We anticipate the ability to precisely control particles in 3D at extremely high flow rates will open up several applications, including the development of ultra-high throughput microflow cytometers and high-resolution separation of rare cells for point of care diagnostics.

scholarly journals High Throughput Viscoelastic Particle Focusing and Separation in Spiral Microchannels

2021 ◽  
Author(s):  
Tharagan Kumar ◽  
Harisha Ramachandraiah ◽  
Sharath Narayana Iyengar ◽  
Indradumna Banerjee ◽  
Gustaf Mårtensson ◽  
...  
Keyword(s):  
High Resolution ◽  
High Throughput ◽  
Separation Efficiency ◽  
Point Of Care ◽  
Outer Wall ◽  
Reynolds Numbers ◽  
Inertial Microfluidics ◽  
Flow Rates ◽  
Particle Focusing ◽  
Sample Flow Rate

Abstract Passive particle manipulation using inertial and elasto-inertial microfluidics have received substantial interest in recent years and have found various applications in high throughput particle sorting and separation. For separation applications, elasto-inertial microfluidics has thus far been applied at substantial lower flow rates as compared to inertial microfluidics. In this work, we explore viscoelastic particle focusing and separation in spiral channels at two orders of magnitude higher Reynolds numbers than previously reported. We show that the balance between dominant inertial lift force, dean drag force and elastic force enables stable 3D particle focusing at dynamically high Reynolds numbers. Using a two-turn spiral, we show that particles, initially pinched towards the inner wall using an elasticity enhancer, PEO (polyethylene oxide), as sheath migrate towards the outer wall strictly based on size and can be effectively separated with high precision. As a proof of principle for high resolution particle separation, 15 µm particles were effectively separated from 10 µm particles. A separation efficiency of 98% for the 10 µm and 97% for the 15µm particles was achieved. Furthermore, we demonstrate sheath-less, high throughput, separation using a novel integrated two-spiral device and achieved a separation efficiency of 89% for the 10 µm and 99% for the 15µm particles at a sample flow rate of 1 mL/ml – a throughput previously only reported for inertial microfluidics. We anticipate the ability to precisely control particles in 3D at extremely high flow rates will open up several applications, including the development of ultra-high throughput microflow cytometers and high-resolution separation of rare cells for point of care diagnostics.

scholarly journals Analogue tuning of particle focusing in elasto-inertial flow

Meccanica ◽  
2021 ◽  
Author(s):  
I. Banerjee ◽  
M. E. Rosti ◽  
T. Kumar ◽  
L. Brandt ◽  
A. Russom
Keyword(s):  
Numerical Simulations ◽  
Cross Section ◽  
Immersed Boundary Method ◽  
Circular Cross Section ◽  
Finite Size ◽  
Reynolds Numbers ◽  
Immersed Boundary ◽  
Particle Focusing ◽  
Inertial Flow ◽  
Particle Behaviour

AbstractWe report a unique tuneable analogue trend in particle focusing in the laminar and weak viscoelastic regime of elasto-inertial flows. We observe experimentally that particles in circular cross-section microchannels can be tuned to any focusing bandwidths that lie between the “Segre-Silberberg annulus” and the centre of a circular microcapillary. We use direct numerical simulations to investigate this phenomenon and to understand how minute amounts of elasticity affect the focussing of particles at increasing flow rates. An Immersed Boundary Method is used to account for the presence of the particles and a FENE-P model is used to simulate the presence of polymers in a Non-Newtonian fluid. The numerical simulations study the dynamics and stability of finite size particles and are further used to analyse the particle behaviour at Reynolds numbers higher than what is allowed by the experimental setup. In particular, we are able to report the entire migration trajectories of the particles as they reach their final focussing positions and extend our predictions to other geometries such as the square cross section. We believe complex effects originate due to a combination of inertia and elasticity in the weakly viscoelastic regime, where neither inertia nor elasticity are able to mask each other’s effect completely, leading to a number of intermediate focusing positions. The present study provides a fundamental new understanding of particle focusing in weakly elastic and strongly inertial flows, whose findings can be exploited for potentially multiple microfluidics-based biological sorting applications.

Inertial focusing and zeta potential measurements of single-nanoparticles using octet-nanochannels

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Shohei Kishimoto ◽  
Makusu Tsutsui ◽  
Kazumichi Yokota ◽  
Masateru Taniguchi
Keyword(s):  
Zeta Potential ◽  
Inertial Effects ◽  
Inertial Focusing ◽  
Particle Focusing ◽  
Single Nanoparticle

Electrokinetics in octet nanochannels was demonstrated to enable particle focusing via inertial effects to accurate single-nanoparticle zeta-potential measurements.

Microfluidic device for sheathless particle focusing and separation using a viscoelastic fluid

2015 ◽  
Vol 1406 ◽  
pp. 244-250 ◽  
Author(s):  
Jeonghun Nam ◽  
Bumseok Namgung ◽  
Chwee Teck Lim ◽  
Jung-Eun Bae ◽  
Hwa Liang Leo ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Viscoelastic Fluid ◽  
Particle Focusing

High-throughput concentration of rare malignant tumor cells from large-volume effusions by multistage inertial microfluidics

Lab on a Chip ◽  
2022 ◽  
Author(s):  
Nan Xiang ◽  
Zhonghua Ni
Keyword(s):  
Tumor Cells ◽  
High Throughput ◽  
Malignant Tumor ◽  
Large Volume ◽  
Concentration Factor ◽  
Pleural Effusions ◽  
Inertial Microfluidics ◽  
Low Concentration ◽  
Malignant Pleural Effusions ◽  
On Chip

On-chip concentration of rare malignant tumor cells (MTCs) in malignant pleural effusions (MPEs) with a large volume is challenging. Previous microfluidic concentrators suffer from a low concentration factor (CF) and...

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