scholarly journals Through-needle all-optical ultrasound imaging in vivo: a preclinical swine study

2017 ◽  
Vol 6 (12) ◽  
pp. e17103-e17103 ◽  
Author(s):  
Malcolm C Finlay ◽  
Charles A Mosse ◽  
Richard J Colchester ◽  
Sacha Noimark ◽  
Edward Z Zhang ◽  
...  
Keyword(s):  
Ultrasound Imaging ◽  
All Optical

scholarly journals Molecular imaging of orthotopic prostate cancer with nanobubble ultrasound contrast agents targeted to PSMA

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yu Wang ◽  
Al Christopher De Leon ◽  
Reshani Perera ◽  
Eric Abenojar ◽  
Ramamurthy Gopalakrishnan ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Ultrasound Imaging ◽  
Prostate Gland ◽  
Ultrasound Contrast Agent ◽  
Tumor Model ◽  
Focal Therapy ◽  
Ultrasound Contrast Agents ◽  
Time Intensity Curve ◽  
Ultrasound Contrast

AbstractUltrasound imaging is routinely used to guide prostate biopsies, yet delineation of tumors within the prostate gland is extremely challenging, even with microbubble (MB) contrast. A more effective ultrasound protocol is needed that can effectively localize malignancies for targeted biopsy or aid in patient selection and treatment planning for organ-sparing focal therapy. This study focused on evaluating the application of a novel nanobubble ultrasound contrast agent targeted to the prostate specific membrane antigen (PSMA-targeted NBs) in ultrasound imaging of prostate cancer (PCa) in vivo using a clinically relevant orthotopic tumor model in nude mice. Our results demonstrated that PSMA-targeted NBs had increased extravasation and retention in PSMA-expressing orthotopic mouse tumors. These processes are reflected in significantly different time intensity curve (TIC) and several kinetic parameters for targeted versus non-targeted NBs or LUMASON MBs. These, may in turn, lead to improved image-based detection and diagnosis of PCa in the future.

scholarly journals A Tumbling Magnetic Microrobot System for Biomedical Applications

Micromachines ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 861
Author(s):  
Elizabeth E. Niedert ◽  
Chenghao Bi ◽  
Georges Adam ◽  
Elly Lambert ◽  
Luis Solorio ◽  
...  
Keyword(s):  
Ultrasound Imaging ◽  
Biomedical Applications ◽  
Imaging System ◽  
Ex Vivo ◽  
Negative Control ◽  
Circular Path ◽  
Rotational Axis ◽  
Significant Difference

A microrobot system comprising an untethered tumbling magnetic microrobot, a two-degree-of-freedom rotating permanent magnet, and an ultrasound imaging system has been developed for in vitro and in vivo biomedical applications. The microrobot tumbles end-over-end in a net forward motion due to applied magnetic torque from the rotating magnet. By turning the rotational axis of the magnet, two-dimensional directional control is possible and the microrobot was steered along various trajectories, including a circular path and P-shaped path. The microrobot is capable of moving over the unstructured terrain within a murine colon in in vitro, in situ, and in vivo conditions, as well as a porcine colon in ex vivo conditions. High-frequency ultrasound imaging allows for real-time determination of the microrobot’s position while it is optically occluded by animal tissue. When coated with a fluorescein payload, the microrobot was shown to release the majority of the payload over a 1-h time period in phosphate-buffered saline. Cytotoxicity tests demonstrated that the microrobot’s constituent materials, SU-8 and polydimethylsiloxane (PDMS), did not show a statistically significant difference in toxicity to murine fibroblasts from the negative control, even when the materials were doped with magnetic neodymium microparticles. The microrobot system’s capabilities make it promising for targeted drug delivery and other in vivo biomedical applications.

In Vivo Ultrasound Imaging Of Pancreatic Islets

2011 ◽  
Vol 165 (2) ◽  
pp. 207-208
Author(s):  
J.L. Paredes ◽  
J. Wang ◽  
F. Villanueva ◽  
G.K. Gittes
Keyword(s):  
Pancreatic Islets ◽  
Ultrasound Imaging

scholarly journals All-optical electrophysiology with improved genetically encoded voltage indicators reveals interneuron network dynamics in vivo

2021 ◽  
Author(s):  
He Tian ◽  
Hunter C. Davis ◽  
J. David Wong-Campos ◽  
Linlin Z. Fan ◽  
Benjamin Gmeiner ◽  
...  
Keyword(s):  
Gap Junction ◽  
Signal To Noise Ratio ◽  
Voltage Imaging ◽  
All Optical ◽  
Precision Voltage ◽  
Coupling Strengths ◽  
Junction Coupling ◽  
Multiple Cells ◽  
Voltage Indicator

All-optical electrophysiology can be a powerful tool for studying neural dynamics in vivo, as it offers the ability to image and perturb membrane voltage in multiple cells simultaneously. The "Optopatch" constructs combine a red-shifted archaerhodopsin (Arch)-derived genetically encoded voltage indicator (GEVI) with a blue-shifted channelrhodopsin actuator (ChR). We used a video-based pooled screen to evolve Arch-derived GEVIs with improved signal-to-noise ratio (QuasAr6a) and kinetics (QuasAr6b). By combining optogenetic stimulation of individual cells with high-precision voltage imaging in neighboring cells, we mapped inhibitory and gap junction-mediated connections, in vivo. Optogenetic activation of a single NDNF-expressing neuron in visual cortex Layer 1 significantly suppressed the spike rate in some neighboring NDNF interneurons. Hippocampal PV cells showed near-synchronous spikes across multiple cells at a frequency significantly above what one would expect from independent spiking, suggesting that collective inhibitory spikes may play an important signaling role in vivo. By stimulating individual cells and recording from neighbors, we quantified gap junction coupling strengths. Together, these results demonstrate powerful new tools for all-optical microcircuit dissection in live mice.

In Vivo Analysis of Intramuscular Gene Transfer in Human Subjects Studied by On-line Ultrasound Imaging

2001 ◽  
Vol 12 (12) ◽  
pp. 1543-1549 ◽  
Author(s):  
Guenter Rauh ◽  
Ann Pieczek ◽  
William Irwin ◽  
Robert Schainfeld ◽  
Jeffrey M. Isner
Keyword(s):  
Gene Transfer ◽  
Ultrasound Imaging ◽  
Human Subjects ◽  
On Line ◽  
In Vivo Analysis

High-Resolution Ultrasound Imaging of Human Skin In Vivo by Using Three-Dimensional Ultrasound Microscopy

2012 ◽  
Vol 38 (10) ◽  
pp. 1833-1838 ◽  
Author(s):  
Kazutoshi Kumagai ◽  
Hideyuki Koike ◽  
Ryo Nagaoka ◽  
Shingo Sakai ◽  
Kazuto Kobayashi ◽  
...  
Keyword(s):  
High Resolution ◽  
Human Skin ◽  
Ultrasound Imaging ◽  
Three Dimensional ◽  
Ultrasound Microscopy
Sign in / Sign up

Export Citation Format

Share Document