scholarly journals Microsecond scale vibrational spectroscopic imaging by multiplex stimulated Raman scattering microscopy

2015 ◽  
Vol 4 (3) ◽  
pp. e265-e265 ◽  
Author(s):  
Chien-Sheng Liao ◽  
Mikhail N Slipchenko ◽  
Ping Wang ◽  
Junjie Li ◽  
Seung-Young Lee ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Spectroscopic Imaging ◽  
Stimulated Raman

scholarly journals Spectrometer-free vibrational imaging by retrieving stimulated Raman signal from highly scattered photons

2015 ◽  
Vol 1 (9) ◽  
pp. e1500738 ◽  
Author(s):  
Chien-Sheng Liao ◽  
Pu Wang ◽  
Ping Wang ◽  
Junjie Li ◽  
Hyeon Jeong Lee ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Collection Efficiency ◽  
Mouse Skin ◽  
Spectroscopic Imaging ◽  
Acquisition Time ◽  
Raman Signal ◽  
Stimulated Raman ◽  
Photon Collection

In vivo vibrational spectroscopic imaging is inhibited by relatively slow spectral acquisition on the second scale and low photon collection efficiency for a highly scattering system. Recently developed multiplex coherent anti-Stokes Raman scattering and stimulated Raman scattering techniques have improved the spectral acquisition time down to microsecond scale. These methods using a spectrometer setting are not suitable for turbid systems in which nearly all photons are scattered. We demonstrate vibrational imaging by spatial frequency multiplexing of incident photons and single photodiode detection of a stimulated Raman spectrum within 60 μs. Compared to the spectrometer setting, our method improved the photon collection efficiency by two orders of magnitude for highly scattering specimens. We demonstrated in vivo imaging of vitamin E distribution on mouse skin and in situ imaging of human breast cancerous tissues. The reported work opens new opportunities for spectroscopic imaging in a surgical room and for development of deep-tissue Raman spectroscopy toward molecular level diagnosis.

In Vivo and in Situ Spectroscopic Imaging by a Handheld Stimulated Raman Scattering Microscope

ACS Photonics ◽  
2017 ◽  
Vol 5 (3) ◽  
pp. 947-954 ◽  
Author(s):  
Chien-Sheng Liao ◽  
Pu Wang ◽  
Chih Yu Huang ◽  
Peng Lin ◽  
Gregory Eakins ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Spectroscopic Imaging ◽  
Stimulated Raman

Ultra-violet Stimulated Raman Scattering of SrWO4 Crystal

2009 ◽  
Vol 24 (3) ◽  
pp. 563-566 ◽  
Author(s):  
Zheng-Ping WANG ◽  
Da-Wei HU ◽  
Huai-Jin ZHANG ◽  
Xin-Guang XU ◽  
Ji-Yang WANG ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Ultra Violet ◽  
Stimulated Raman

Bulk Laser Damage Threshold and Stimulated Raman Scattering in KTiOPO4 Crystal.

1996 ◽  
Vol 24 (8) ◽  
pp. 906-909
Author(s):  
Akio MIYAMOTO ◽  
Hidetsugu YOSHIDA ◽  
Yusuke MORI ◽  
Takatomo SASAKI ◽  
Sadao NAKAI
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Damage Threshold ◽  
Laser Damage Threshold ◽  
Laser Damage ◽  
Stimulated Raman

scholarly journals Utilizing Stimulated Raman Scattering Microscopy To Study Intracellular Distribution of Label-Free Ponatinib in Live Cells

2019 ◽  
Vol 63 (5) ◽  
pp. 2028-2034 ◽  
Author(s):  
Kristel Sepp ◽  
Martin Lee ◽  
Marie T. J. Bluntzer ◽  
G. Vignir Helgason ◽  
Alison N. Hulme ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Intracellular Distribution ◽  
Live Cells ◽  
Label Free ◽  
Stimulated Raman
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