scholarly journals Impact of serum storage conditions on microRNA stability

Leukemia ◽  
2012 ◽  
Vol 26 (11) ◽  
pp. 2414-2416 ◽  
Author(s):  
S Grasedieck ◽  
N Schöler ◽  
M Bommer ◽  
J H Niess ◽  
H Tumani ◽  
...  
Keyword(s):  
Storage Conditions ◽  
Serum Storage

scholarly journals Altered Distribution of Transferrin Isoforms According to Serum Storage Conditions

2005 ◽  
Vol 51 (11) ◽  
pp. 2159-2162 ◽  
Author(s):  
Brice MR Appenzeller ◽  
Robert Wennig
Keyword(s):  
Storage Conditions ◽  
Serum Storage ◽  
Transferrin Isoforms

scholarly journals Effect of pig serum storage conditions on detection of anti-ASFV antibodies by ELISA

2021 ◽  
pp. 216-223
Author(s):  
A. R. Shotin ◽  
I. Yu. Zhukov ◽  
A. S. Pershin ◽  
Ali Mazloum ◽  
I. V. Shevchenko ◽  
...  
Keyword(s):  
Shelf Life ◽  
African Swine Fever ◽  
Storage Conditions ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Published Data ◽  
Storage Duration ◽  
Serological Status ◽  
Serum Storage

One of the measures used to control and prevent African swine fever spread in the Russian Federation involves testing pig and boar sera using inter alia serological tools based on enzyme-linked immunosorbent assay (ELISA) for anti-viral antibody detection. However, there is no unified regulatory document specifying storage conditions for sera used in the ELISA for anti-ASFV antibody detection. There are also lack of published data on the maximum admissible shelf life of the pig sera, and the effect of storage conditions on the serological status of the pig sera as for ASF is understudied. The paper demonstrates results of the experiment aimed at the determination of the effect of storage temperatures and shelf life on the serological status of ASFV seropositive and seronegative pig sera when tested by INgezim PPA Compac (Ingenasa, Spain) ELISA as well as on the possibility of false results. During the experiment and analysis of its results, the new data were obtained, and they indicated from none to non-significant effect of the simulated storage conditions on the serological status of sera used for ASFV detection, while hemolyzed sera demonstrated more significant changes proportional to hemolysis degree and storage duration. Although the results of detection of antibodies against the agents of some diseases cannot be used in case of other pathogens, this study has a substantial applied significance as it allows to specify the dependence of the valid results of ASF serodiagnosis on the storage conditions of the samples.

Evaluation of Microbial Load from Canned Soya Milk Drinks in Malaysia

2016 ◽  
Vol 2 (1) ◽  
pp. 22-25
Author(s):  
Nur Amalina binti Mustafa ◽  
Muhammad Ashraf bin Redzuan ◽  
Muhamad Hazim bin Zuraimi ◽  
Muhamad Shuhaimi bin Shuib ◽  
Shahnaz Majeed ◽  
...  
Keyword(s):  
Culture Media ◽  
Storage Condition ◽  
Storage Conditions ◽  
Nutrient Agar ◽  
Microbial Load ◽  
Blood Agar ◽  
Human Consumption ◽  
Processing Unit ◽  
Soya Milk ◽  
Two Samples

Objective: Owing to the habit of consuming ready food among the citizens of Malaysia a study was conducted to evaluate 20 samples of canned soya milk for the presence of possible microbial content. The samples were collected randomly from shopping malls, restaurants and kiosk in Ipoh Malaysia. Methods: All samples collected across Ipoh, were subjected to test for presence bacteria in nutrient agar, blood agar and macConkey media. The possible microbial load was swapped from surface and soya milk content with a sterile cotton and streaked on nutrient agar, blood agar and macConkey culture media. The streaked petri plates were incubated for 48 hours at 37oC. Results: The study revealed negative microbial growth in all except two samples from the surface and soya milk content collected from a restaurant in nutrient agar and blood agar medium. The presence of microbes was conformed as gram positive staphylococcus sp. through gram staining. The positive growth may be imputed to poor storage condition at the restaurant. Conclusion: It can be computed from the study that the majority of the samples were free from bacterial growth, suggesting strong in house quality control mechanism at the processing unit and exquisite storage conditions in malls and kiosk suggesting that soya milk available in malls and kiosk are fit for human consumption.

FORMULATION AND DEVELOPMENT OF FAST DISSOLVING TABLET OF METOCLOPRAMIDE: AN ANTI-EMETIC DRUG

2019 ◽  
Vol 8 (6) ◽  
Author(s):  
Avilash Carpenter ◽  
M.K. Gupta ◽  
Neetesh Kumar Jain ◽  
Urvashi Sharma ◽  
Rahul Sisodiya
Keyword(s):  
Mechanical Strength ◽  
Standard Procedure ◽  
Flow Property ◽  
Storage Conditions ◽  
Angle Of Repose ◽  
Dissolution Time ◽  
Disintegration Time ◽  
Powder Blend ◽  
Standard Curve ◽  
The Stability

Aim: The main of the study is to formulate and develop orally disintegrating fast dissolving tablet of Metoclopramide hydrochloride. Material & Methods: Before formulation and development of selected drug, the standard curve in buffer was prepared and absorbance at selected maxima was taken. Then two different disintegrating agents were selected and drug was mixed with disintegrating agents in different ratio. Various Preformulation parameters and evaluation of tablet i.e. disintegration time, dissolution time, friability, hardness, thickness were measured by standard procedure. Result & Discussion: The angle of repose for all the batches prepared. The values were found to be in the range of 30.46 to 36.45, which indicates good flow property for the powder blend according to the USP. The bulk density and tapped density for all the batches varied from 0.49 to 0.54 g/mL and 0.66 to 0.73, respectively. Carr’s index values were found to be in the range of 23.33 to 25.88, which is satisfactory for the powders as well as implies that the blends have good compressibility. Hausner ratio values obtained were in the range of 1.22 to 1.36, which shows a passable flow property for the powder blend based on the USP. The results for tablet thickness and height for all batches was found to range from 4.45 to 4.72 mm and 3.67 to 3.69 mm, respectively. Hardness or breaking force of tablets for all batches was found to range from 32.8 to 36.2 N. Tablet formulations must show good mechanical strength with sufficient hardness in order to handle shipping and transportation. Friability values for all the formulations were found to be in the range of 0.22 % to 0.30 %. Conclusion: Orally disintegrating tablets were compressed in order to have sufficient mechanical strength and integrity to withstand handling, shipping and transportation. The formulation was shown to have a rapid disintegration time that complied with the USP (less than one minute). The data obtained from the stability studies indicated that the orally disintegrating mini-tablets of MTH were stable under different environmental storage conditions. Keywords: Formulation & Development, Fast Dissolving Tablet, Metoclopramide, Anti-Emetic Drug, Oral Disintegrating Tablet

METHODOLOGICAL STUDY OF THE RADIOIMMUNOASSAY OF HUMAN THYROTROPHIN

1972 ◽  
Vol 71 (1) ◽  
pp. 24-36 ◽  
Author(s):  
Ariel Gordin ◽  
Pirkko Saarinen
Keyword(s):  
Healthy Subjects ◽  
Binding Capacity ◽  
Paper Electrophoresis ◽  
Storage Conditions ◽  
Methodological Study ◽  
Double Antibody ◽  
The Mean ◽  
Specific Activities ◽  
Initial Binding ◽  
In Pregnancy

ABSTRACT An account is given of a methodological study of the double-antibody radioimmunoassay of human TSH, using highly purified labelled human TSH as tracer. It was shown that conventional paper electrophoresis was not adequate for studying the purity of labelled human TSH. When polyvinylchloride (Pevikon®) electrophoresis was used, four subfractions could still be separated, even though, on paper electrophoresis, the material seemed to be homogeneous. Only two of the four Pevikon fractions were immunoreactive. Purification of labelled human TSH by Pevikon electrophoresis also improved the sensitivity of the assay. Specific activities of about 100 mCi/mg gave the highest initial binding capacity, produced least damage to the labelled hormone and showed the best stability of the tracer without influencing the sensitivity of the method. In different storage conditions, labelled human TSH was found to be most stable at −20°C and diluted 1/100. Only in pregnancy did the addition of HCG seem necessary. The mean TSH value in healthy subjects was 3.6 ± 1.4 μU/ml (mean±sd) with a range from 1.6 μU/ml to 8.8 μU/ml.

Safeguarding National Heritage through Conservation of Oral History Collections at Bulawayo National Archives, Zimbabwe

2019 ◽  
Vol 6 (2) ◽  
Author(s):  
Vimbayi Natalie Nhenga-Mugarisanwa ◽  
Peterson Dewah
Keyword(s):  
Oral History ◽  
Minority Groups ◽  
Case Study Research ◽  
Storage Conditions ◽  
Annual Reports ◽  
Conservation Unit ◽  
National Heritage ◽  
National Archives ◽  
Digital Formats ◽  
Depth Interviews

 Oral history collections are vital assets that represent national heritage. While transcribed collections are kept in a proper archival repository at the Bulawayo National Archives, the audio-visual collections are kept unprocessed and unprotected in unsuitable housing such as cardboard boxes within the Principal Archivist’s office. Storage conditions are not conducive and therefore, unbearable. The study, therefore, explored issues relating to how national heritage contained in oral history collections can be protected through conservation at the institution. In this regard, the study opted to employ the qualitative research methodology, using the case study research design. The informants were purposively sampled, while data were collected through questionnaires, in-depth interviews, and document analysis. Questionnaires were administered to the chosen respondents through hand delivery. Researchers conducted in-depth interviews that lasted between 20–30 minutes. The Director’s Annual Reports from 1990 to 2016 were analysed for the study. The findings of the study indicated that the Bulawayo National Archives captures and stores oral history in paper, cassettes, magnetic tape, and digital formats, and according to various subject areas; which include chieftaincy, minority groups, land question, religion and liberation wars. However, the institution does not have a conservation unit nor an Oral Historian in charge of the collections. In this regard, we recommend that the institution sets up a conservation unit to protect oral history collections, and facilitate the restoration of the already damaged and deteriorated oral history collections. 

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