Cell Culture Models and Animal Models of Viral Hepatitis. Part II: Hepatitis C

Lab Animal ◽  
2005 ◽  
Vol 34 (2) ◽  
pp. 39-47 ◽  
Author(s):  
Chandan Guha ◽  
Sung-W Lee ◽  
Namita Roy Chowdhury ◽  
Jayanta Roy Chowdhury
Keyword(s):  
Cell Culture ◽  
Hepatitis C ◽  
Animal Models ◽  
Viral Hepatitis ◽  
Culture Models ◽  
Cell Culture Models

scholarly journals Ribavirin potentiates interferon action by augmenting interferon-stimulated gene induction in hepatitis C virus cell culture models

Hepatology ◽  
2010 ◽  
Vol 53 (1) ◽  
pp. 32-41 ◽  
Author(s):  
Emmanuel Thomas ◽  
Jordan J. Feld ◽  
Qisheng Li ◽  
Zongyi Hu ◽  
Michael W. Fried ◽  
...  
Keyword(s):  
Cell Culture ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Gene Induction ◽  
Culture Models ◽  
Cell Culture Models

scholarly journals Factors to consider when interrogating 3D culture models with plate readers or automated microscopes

2021 ◽  
Author(s):  
Terry Riss ◽  
O. Joseph Trask
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
3D Culture ◽  
Fluorescent Imaging ◽  
Culture Models ◽  
Assay Methods ◽  
Diffusion Rates ◽  
Cell Culture Models ◽  
Dimensional Cell ◽  
Cells Cultured

AbstractAlong with the increased use of more physiologically relevant three-dimensional cell culture models comes the responsibility of researchers to validate new assay methods that measure events in structures that are physically larger and more complex compared to monolayers of cells. It should not be assumed that assays designed using monolayers of cells will work for cells cultured as larger three-dimensional masses. The size and barriers for penetration of molecules through the layers of cells result in a different microenvironment for the cells in the outer layer compared to the center of three-dimensional structures. Diffusion rates for nutrients and oxygen may limit metabolic activity which is often measured as a marker for cell viability. For assays that lyse cells, the penetration of reagents to achieve uniform cell lysis must be considered. For live cell fluorescent imaging assays, the diffusion of fluorescent probes and penetration of photons of light for probe excitation and fluorescent emission must be considered. This review will provide an overview of factors to consider when implementing assays to interrogate three dimensional cell culture models.

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