scholarly journals Alternatively spliced isoforms of WT1 control podocyte-specific gene expression

2015 ◽  
Vol 88 (2) ◽  
pp. 321-331 ◽  
Author(s):  
Jonathan Lefebvre ◽  
Michael Clarkson ◽  
Filippo Massa ◽  
Stephen T. Bradford ◽  
Aurelie Charlet ◽  
...  
Keyword(s):  
Gene Expression ◽  
Specific Gene ◽  
Specific Gene Expression ◽  
Alternatively Spliced

scholarly journals Interaction of Ets-1 and the POU-homeodomain protein GHF-1/Pit-1 reconstitutes pituitary-specific gene expression.

1997 ◽  
Vol 17 (3) ◽  
pp. 1065-1074 ◽  
Author(s):  
A P Bradford ◽  
C Wasylyk ◽  
B Wasylyk ◽  
A Gutierrez-Hartmann
Keyword(s):  
Gene Expression ◽  
Specific Protein ◽  
Protein Domain ◽  
Specific Gene ◽  
Homeodomain Protein ◽  
Specific Expression ◽  
Specific Gene Expression ◽  
Cis Element ◽  
Alternatively Spliced ◽  
Combinatorial Interactions

The pituitary-specific, POU-homeodomain factor GHF-1/Pit-1 is necessary, but not sufficient, for cell-specific expression of prolactin (PRL), growth hormone (GH), and thyrotropin. Combinatorial interactions of GHF-1 with other factors are likely to be required; however, such factors and their mechanisms of action remain to be elucidated. Here we identify Ets-1 as a factor that functionally and physically interacts with GHF-1 to fully reconstitute proximal PRL promoter activity. In contrast, Ets-2 has no effect, and the alternatively spliced GHF-2/Pit-1beta variant fails to synergize with Ets-1. The Ets-1-GHF-1 synergy requires a composite Ets-1-GHF-1 cis element and is dependent on an Ets-1-specific protein domain. Furthermore, the ancestrally related and GHF-1-dependent GH promoter, which lacks this composite element, does not exhibit this response. Finally, Ets-1, but not Ets-2, binds directly to GHF-1 and GHF-2. These data show that a functional interaction of GHF-1 and Ets-1, acting via a composite DNA element, is required to establish lactotroph-specific PRL gene expression, thus providing a molecular mechanism by which GHF-1 can discriminate between the GH and PRL genes. These results underscore the importance of transcription factors that are distinct from, but interact with, homeobox proteins to establish lineage-specific gene expression.

scholarly journals Role of cyclic AMP in the control of cell-specific gene expression

1993 ◽  
Vol 4 (6) ◽  
pp. 204-209 ◽  
Author(s):  
Wolfgang Schmid ◽  
Doris Nitsch ◽  
Michael Boshart ◽  
Günther Schütz
Keyword(s):  
Gene Expression ◽  
Cyclic Amp ◽  
Specific Gene ◽  
Specific Gene Expression
Sign in / Sign up

Export Citation Format

Share Document