scholarly journals Alternatively activated macrophages as therapeutic agents for kidney disease: in vivo stability is a key factor

2014 ◽  
Vol 85 (4) ◽  
pp. 730-733 ◽  
Author(s):  
Senthilkumar Alagesan ◽  
Matthew D. Griffin
Keyword(s):  
Kidney Disease ◽  
Therapeutic Agents ◽  
Activated Macrophages ◽  
Alternatively Activated Macrophages ◽  
Key Factor ◽  
Alternatively Activated

scholarly journals FIZZ1 and Ym as Tools to Discriminate between Differentially Activated Macrophages

2002 ◽  
Vol 9 (3) ◽  
pp. 151-159 ◽  
Author(s):  
Geert Raes ◽  
Wim Noël ◽  
Alain Beschin ◽  
Lea Brys ◽  
Patrick de Baetselier ◽  
...  
Keyword(s):  
Mouse Strains ◽  
Molecular Characteristics ◽  
Activated Macrophages ◽  
Alternatively Activated Macrophages ◽  
Different Populations ◽  
Classically Activated Macrophages ◽  
Macrophage Populations ◽  
Alternatively Activated

Although it is well-established that macrophages can occur in distinct activation states, the molecular characteristics of differentially activated macrophages, and particularly those of alternatively activated macrophages (aaMφ), are still poorly unraveled. Recently, we demonstrated that the expression of FIZZ1 and Ym is induced in aaMφ as compared with classically activated macrophages (caMφ), elicitedin vitroor developedin vivoduring infection withTrypanosoma brucei brucei. In the present study, we analyzed the expression of FIZZ1 and Ym in caMφ and aaMφ elicited duringTrypanosoma congolenseinfection and show that the use of FIZZ1 and Ym for the identification of aaMφ is not limited toT. b. bruceiinfection and is independent of the organ sources from which macrophages are obtained. We also demonstrate that FIZZ1 can be used to discriminate between different populations of aaMφ. Furthermore, we studied the effects of various stimuli, and combinations thereof, on the expression of FIZZ1 and Ym in macrophages from different mouse strains and demonstrate that regulation of the expression of FIZZ1 and Ym in macrophages is not dependent on the mouse strain. Finally, we show that these genes can be used to monitor the macrophage activation status without the need to obtain pure macrophage populations.

scholarly journals Interdependence between Chromogranin-A, Alternatively Activated Macrophages, Tight Junction Proteins and the Epithelial Functions. A Human and In-Vivo/In-Vitro Descriptive Study

2020 ◽  
Vol 21 (21) ◽  
pp. 7976
Author(s):  
Nour Eissa ◽  
Hayam Hussein ◽  
Diane M. Tshikudi ◽  
Geoffrey N. Hendy ◽  
Charles N. Bernstein ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Mrna Expression ◽  
Chromogranin A ◽  
Intestinal Inflammation ◽  
Collagen Deposition ◽  
Activated Macrophages ◽  
Alternatively Activated Macrophages ◽  
Alternatively Activated

Background: Ulcerative colitis (UC) is characterized by altered chromogranin-A (CHGA), alternatively activated macrophages (M2) and intestinal epithelial cells (IECs). We previously demonstrated that CHGA is implicated in colitis progression by regulating the macrophages. Here, we investigated the interplay between CHGA, M2, tight junctions (TJ) and IECs in an inflammatory environment. Methods: Correlations between CHGA mRNA expression of and TJ proteins mRNA expressions of (Occludin [OCLN], zonula occludens-1 [ZO1], Claudin-1 [CLDN1]), epithelial associated cytokines (interleukin [IL]-8, IL-18), and collagen (COL1A2) were determined in human colonic mucosal biopsies isolated from active UC and healthy patients. Acute UC-like colitis (5% dextran sulphate sodium [DSS], five days) was induced in Chga-C57BL/6-deficient (Chga−/−) and wild type (Chga+/+) mice. Col1a2 TJ proteins, Il-18 mRNA expression and collagen deposition were determined in whole colonic sections. Naïve Chga−/− and Chga+/+ peritoneal macrophages were isolated and exposed six hours to IL-4/IL-13 (20 ng/mL) to promote M2 and generate M2-conditioned supernatant. Caco-2 epithelial cells were cultured in the presence of Chga−/− and Chga+/+ non- or M2-conditioned supernatant for 24 h then exposed to 5% DSS for 24 h, and their functional properties were assessed. Results: In humans, CHGA mRNA correlated positively with COL1A2, IL-8 and IL-18, and negatively with TJ proteins mRNA markers. In the experimental model, the deletion of Chga reduced IL-18 mRNA and its release, COL1A2 mRNA and colonic collagen deposition, and maintained colonic TJ proteins. Chga−/− M2-conditioned supernatant protected caco-2 cells from DSS and oxidative stress injuries by improving caco-2 cells functions (proliferation, viability, wound healing) and by decreasing the release of IL-8 and IL-18 and by maintaining the levels of TJ proteins, and when compared with Chga+/+ M2-conditioned supernatant. Conclusions: CHGA contributes to the development of intestinal inflammation through the regulation of M2 and epithelial cells. Targeting CHGA may lead to novel biomarkers and therapeutic strategies in UC.

Early removal of alternatively activated macrophages leads to Taenia crassiceps cysticercosis clearance in vivo

2010 ◽  
Vol 40 (6) ◽  
pp. 731-742 ◽  
Author(s):  
José L. Reyes ◽  
César A. Terrazas ◽  
Javier Alonso-Trujillo ◽  
Nico van Rooijen ◽  
Abhay R. Satoskar ◽  
...  
Keyword(s):  
Activated Macrophages ◽  
Taenia Crassiceps ◽  
Alternatively Activated Macrophages ◽  
Early Removal ◽  
Alternatively Activated

scholarly journals Alternatively Activated Macrophages Are the Primary Retinoic Acid-Producing Cells in Human Decidua

2020 ◽  
Vol 27 (1) ◽  
pp. 334-341 ◽  
Author(s):  
Augustine Rajakumar ◽  
Maureen A. Kane ◽  
Jianshi Yu ◽  
Jace W. Jones ◽  
Hongyan Qu ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Activated Macrophages ◽  
Alternatively Activated Macrophages ◽  
Human Decidua ◽  
Alternatively Activated

Allergic airway disease is unaffected by the absence of IL-4Rα–dependent alternatively activated macrophages

2012 ◽  
Vol 130 (3) ◽  
pp. 743-750.e8 ◽  
Author(s):  
Natalie E. Nieuwenhuizen ◽  
Frank Kirstein ◽  
Jaisubash Jayakumar ◽  
Babele Emedi ◽  
Ramona Hurdayal ◽  
...  
Keyword(s):  
Airway Disease ◽  
Allergic Airway Disease ◽  
Activated Macrophages ◽  
Alternatively Activated Macrophages ◽  
Alternatively Activated
Sign in / Sign up

Export Citation Format

Share Document