scholarly journals Vasopressin-dependent coupling between sodium transport and water flow in a mouse cortical collecting duct cell line

2011 ◽  
Vol 79 (8) ◽  
pp. 843-852 ◽  
Author(s):  
Hans-Peter Gaeggeler ◽  
Yann Guillod ◽  
Dominique Loffing-Cueni ◽  
Johannes Loffing ◽  
Bernard C. Rossier
Keyword(s):  
Cell Line ◽  
Water Flow ◽  
Sodium Transport ◽  
Collecting Duct ◽  
Duct Cell ◽  
Cortical Collecting Duct

Vasopressin Regulates Water Flow in a Rat Cortical Collecting Duct Cell Line Not Containing Known Aquaporins

2001 ◽  
Vol 179 (1) ◽  
pp. 63-70 ◽  
Author(s):  
C. Capurro ◽  
V. Rivarola ◽  
A. Kierbel ◽  
B. Escoubet ◽  
N. Farman ◽  
...  
Keyword(s):  
Cell Line ◽  
Water Flow ◽  
Collecting Duct ◽  
Duct Cell ◽  
Cortical Collecting Duct

scholarly journals Activation of the Amiloride-Sensitive Epithelial Sodium Channel by the Serine Protease mCAP1 Expressed in a Mouse Cortical Collecting Duct Cell Line

2000 ◽  
Vol 11 (5) ◽  
pp. 828-834
Author(s):  
GRÉGOIRE VUAGNIAUX ◽  
VÉRONIQUE VALLET ◽  
NICOLE FOWLER JAEGER ◽  
CORINNE PFISTER ◽  
MARCELLE BENS ◽  
...  
Keyword(s):  
Sodium Channel ◽  
Cell Line ◽  
Serine Protease ◽  
Sodium Transport ◽  
Serine Proteases ◽  
Collecting Duct ◽  
Short Circuit ◽  
Short Circuit Current ◽  
Duct Cell ◽  
Cortical Collecting Duct

Abstract. This study examines whether serine proteases can activate the amiloride-sensitive sodium channel (ENaC) in mammalian kidney epithelial cells. The transepithelial sodium transport assessed by amiloride-sensitive short-circuit current appears to be sensitive to aprotinin, a protease inhibitor in a mouse cortical collecting duct cell line (mpkCCDc14). This result indicated that serine proteases may be implicated in the regulation of ENaC-mediated sodium transport. Using degenerated oligonucleotides to a previously isolated serine protease from Xenopus, xCAP1 (channel activating protease), a novel full-length serine protease (mCAP1), has been isolated and characterized. RNA analysis showed a broad pattern of expression in tissues (kidney, lung, colon, and salivary glands) expressing ENaC. Reverse transcription-PCR experiments also showed that mCAP1 was abundantly expressed in proximal tubule cells and was also expressed in intact and cultured collecting duct cells. Coexpression of the Xenopus, rat, or human α-, β-, and γ-ENaC subunits in Xenopus oocytes also showed that mCAP1 induces a significant increase in ENaC-mediated current accompanied by a decrease of channel molecules at the cell surface. It is proposed that this novel mouse channel activating protease may act as a regulator of ENaC within the kidney.

scholarly journals Effects of chemical chaperones on partially retarded NaCl cotransporter mutants associated with Gitelman's syndrome in a mouse cortical collecting duct cell line

2004 ◽  
Vol 19 (5) ◽  
pp. 1069-1076 ◽  
Author(s):  
J. C. de Jong ◽  
P. H. G. M. Willems ◽  
M. Goossens ◽  
A. Vandewalle ◽  
L. P. W. J. van den Heuvel ◽  
...  
Keyword(s):  
Cell Line ◽  
Collecting Duct ◽  
Duct Cell ◽  
Cortical Collecting Duct ◽  
Chemical Chaperones ◽  
Gitelman's Syndrome ◽  
Nacl Cotransporter

scholarly journals Transcriptome of a mouse kidney cortical collecting duct cell line: Effects of aldosterone and vasopressin

2001 ◽  
Vol 98 (5) ◽  
pp. 2712-2716 ◽  
Author(s):  
M. Robert-Nicoud ◽  
M. Flahaut ◽  
J.-M. Elalouf ◽  
M. Nicod ◽  
M. Salinas ◽  
...  
Keyword(s):  
Cell Line ◽  
Collecting Duct ◽  
Duct Cell ◽  
Mouse Kidney ◽  
Cortical Collecting Duct

The isolated C-terminus of polycystin-1 promotes increased ATP-stimulated chloride secretion in a collecting duct cell line

2003 ◽  
Vol 104 (3) ◽  
pp. 217-221 ◽  
Author(s):  
Kimberly M. HOOPER ◽  
Robert J. UNWIN ◽  
Michael SUTTERS
Keyword(s):  
Fusion Protein ◽  
Cell Line ◽  
Collecting Duct ◽  
Basolateral Membrane ◽  
Duct Cell ◽  
Chloride Secretion ◽  
Cortical Collecting Duct ◽  
Membrane Expression ◽  
Fusion Protein Expression ◽  
C Terminus

Cyst expansion in autosomal dominant polycystic kidney disease (ADPKD) requires accumulation of fluid into the cyst lumen, which is probably driven by aberrant chloride secretion by the cyst lining epithelium. Extracellular ATP is a potent stimulus for chloride secretion in many epithelial systems, and provides a plausible mechanism for secretion in ADPKD. Therefore the link between polycystin-1 and ATP-stimulated chloride secretion was investigated in the M1 cortical collecting duct cell line. M1 cells were stably transfected with a glucocorticoid-inducible cytoplasmic C-terminal polycystin-1 construct fused to a membrane expression cassette. Induction of fusion protein expression was associated with augmentation of ATP-stimulated transepithelial chloride secretion. After nystatin-induced permeabilization of the basolateral membrane, it was determined that expression of the polycystin fusion protein modulated an ATP-responsive apical chloride conductance. It is concluded that up-regulation of ATP-stimulated chloride secretion might play a significant role in cyst expansion in ADPKD.

scholarly journals Corticosteroid-Dependent Sodium Transport in a Novel Immortalized Mouse Collecting Duct Principal Cell Line

1999 ◽  
Vol 10 (5) ◽  
pp. 923-934
Author(s):  
MARCELLE BENS ◽  
VÉRONIQUE VALLET ◽  
FRANÇOISE CLUZEAUD ◽  
LAURENT PASCUAL-LETALLEC ◽  
AXEL KAHN ◽  
...  
Keyword(s):  
Cell Line ◽  
Sodium Transport ◽  
Collecting Duct ◽  
Short Circuit ◽  
T Antigen ◽  
Sodium Balance ◽  
Sodium Reabsorption ◽  
Cortical Collecting Duct ◽  
Sv40 Large T Antigen ◽  
Corticosteroid Hormones

Abstract. The final control of sodium balance takes place in the cortical collecting duct (CCD) of the nephron, where corticosteroid hormones regulate sodium reabsorption by acting through mineralocorticoid (MR) and/or glucocorticoid (GR) receptors. A clone of principal CCD cells (mpkCCDc14) has been established that is derived from a transgenic mouse (SV40 large T antigen under the control of the SV40 enhancer/L-type pyruvate kinase promoter). Cells grown on filters form polarized monolayers with high electrical transepithelial resistance (RT approximately 4700 Ω × cm2) and potential difference (PD approximately -50 mV) and have an amiloride-sensitive electrogenic sodium transport, as assessed by the short-circuit current method (Isc approximately 11 μA/cm2). Reverse transcription-PCR experiments using rat MR primers, [3H]aldosterone, and [3H]dexamethasone binding and competition studies indicated that the mpkCCDc14 cells exhibit specific MR and GR. Aldosterone increased Isc in a dose- (10-10 to 10-6 M) and time-dependent (2 to 72 h) manner, whereas corticosterone only transiently increased Isc (2 to 6 h). Consistent with the expression of 11β-hydroxysteroid dehydrogenase type 2, which metabolizes glucocorticoids to inactive 11-dehydroderivates, carbenoxolone potentiated the corticosterone-stimulated Isc. Aldosterone (5 × 10-7 M)-induced Isc (fourfold) was associated with a three- to fivefold increase in α-ENaC mRNA (but not in those for β- or γ-ENaC) and three- to 10-fold increases in α-ENaC protein synthesis. In conclusion, this new immortalized mammalian CCD clonal cell line has retained a high level of epithelial differentiation and sodium transport stimulated by aldosterone and therefore represents a useful mammalian cell system for identifying the genes controlled by aldosterone.

scholarly journals Receptors for natriuretic peptides in a human cortical collecting duct cell line

1997 ◽  
Vol 51 (1) ◽  
pp. 281-287 ◽  
Author(s):  
Valerie Millul ◽  
Nicole Ardaillou ◽  
Sandrine Placier ◽  
Béatrice Baudouin ◽  
Pierre M. Ronco
Keyword(s):  
Cell Line ◽  
Natriuretic Peptides ◽  
Collecting Duct ◽  
Duct Cell ◽  
Cortical Collecting Duct

scholarly journals Characteristics of a rat cortical collecting duct cell line that maintains high transepithelial resistance

1996 ◽  
Vol 50 (2) ◽  
pp. 367-376 ◽  
Author(s):  
Marcel Blot-Chabaud ◽  
Monique Laplace ◽  
Françoise Cluzeaud ◽  
Claudia Capurro ◽  
Roland Cassingéna ◽  
...  
Keyword(s):  
Cell Line ◽  
Collecting Duct ◽  
Duct Cell ◽  
Transepithelial Resistance ◽  
Cortical Collecting Duct
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