scholarly journals Establishment of renal stem/progenitor-like cell line from S3 segment of proximal tubules in adult rat kidney

2005 ◽  
Vol 68 (5) ◽  
pp. 1966
Author(s):  
Shinji Kitamura ◽  
Yasushi Yamasaki ◽  
Hirofumi Makino
Keyword(s):  
Cell Line ◽  
Rat Kidney ◽  
Proximal Tubules ◽  
Adult Rat ◽  
S3 Segment

Establishment of renal stem/progenitor-like cell line from S3 segment of proximal tubules in adult rat kidney

2005 ◽  
Vol 68 (5) ◽  
pp. 1966-1966
Author(s):  
Shinji Kitamura ◽  
Yasushi Yamasaki ◽  
Hirofumi Makino
Keyword(s):  
Cell Line ◽  
Rat Kidney ◽  
Proximal Tubules ◽  
Adult Rat ◽  
S3 Segment

scholarly journals Absence of aquaporin-4 water channels from kidneys of the desert rodent Dipodomys merriami merriami

2001 ◽  
Vol 280 (5) ◽  
pp. F794-F802 ◽  
Author(s):  
Yan Huang ◽  
Randall Tracy ◽  
Glenn E. Walsberg ◽  
Anthony Makkinje ◽  
Pingke Fang ◽  
...  
Keyword(s):  
Plasma Membranes ◽  
Rat Kidney ◽  
Freeze Fracture ◽  
Proximal Tubules ◽  
Aquaporin 4 ◽  
Urinary Concentration ◽  
Dipodomys Merriami ◽  
Kangaroo Rat ◽  
S3 Segment ◽  
Aqp4 Protein

Recently, we found that aquaporin-4 (AQP4) is expressed in the S3 segment of renal proximal tubules of mice but not in rat proximal tubules. Because mice have relatively larger papillae than rats, it was proposed that the renal distribution of AQP4 in various species could be related to their maximum urinary concentrating ability. Therefore, kidneys and other tissues of Merriam's desert kangaroo rat, Dipodomys merriami merriami, which produce extremely concentrated urine (up to 5,000 mosmol/kgH2O), were examined for AQP4 expression and localization. Contrary to our expectation, AQP4 immunostaining was undetectable in any region of the kidney, and the absence of AQP4 protein was confirmed by Western blotting. By freeze fracture electron microscopy, orthogonal arrays of intramembraneous particles (OAPs) were not detectable in plasma membranes of principal cells and proximal tubules. However, AQP4 protein was readily detectable in gastric parietal and brain astroglial cells. Northern blotting failed to detect AQP4 mRNA in kangaroo rat kidneys, whereas both in situ hybridization and RT-PCR experiments did reveal AQP4 mRNA in collecting ducts and proximal tubules of the S3 segment. These results suggest that renal expression of AQP4 in the kangaroo rat kidney is regulated at the transcriptional or translational level, and the absence of AQP4 may be critical for the extreme urinary concentration that occurs in this species.

scholarly journals Quantitative immunoelectron microscopic analysis of the localization and induction of 25-hydroxyvitamin D3 24-hydroxylase in rat kidney.

1995 ◽  
Vol 43 (3) ◽  
pp. 255-262 ◽  
Author(s):  
K Iwata ◽  
A Yamamoto ◽  
S Satoh ◽  
Y Ohyama ◽  
Y Tashiro ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Vitamin D3 ◽  
Particle Density ◽  
Rat Kidney ◽  
Microscopic Analysis ◽  
Proximal Tubules ◽  
Gold Particles ◽  
Immunogold Technique ◽  
S3 Segment ◽  
Ultrathin Sections

25-Hydroxyvitamin D3 24(R)-hydroxylase (24-hydroxylase) is involved in the metabolism and regulation of vitamin D3 and is markedly induced by administration of vitamin D3. We detected this enzyme by electron microscopy and an immunogold technique along nephrons of normal and vitamin D3-administered rats. After the rats were administered vitamin D3, 50,000 IU/day for 1 week, they were perfusion-fixed with a paraformaldehyde solution. The fixed kidneys were then removed and embedded in LR White resin. Ultrathin sections were prepared and labeled by the immunogold technique using a mouse anti-rat 25-hydroxyvitamin D3 24-hydroxylase monoclonal antibody. We counted the number of gold particles bound per micron 2 of the mitochondria (particle density) of the tubule epithelial cells along the nephrons. In normal and vitamin D3-administered rats, gold particles were observed in the mitochondria of epithelial cells along the tubules. In normal rats, gold labeling for 24-hydroxylase was statistically significant (p < 0.05), in the S1-S2 segments, the S3 segment of the proximal tubules, and in the distal convoluted tubules. In the rats administered vitamin D3, the particle density increased significantly (p < 0.05) by about 12-fold in the S1-S2 segments of the proximal tubules, whereas it increased less markedly in other parts of the nephron. The marked induction of the S1-S2 segments of the proximal tubules suggests that these segments play an important role in the regulation of vitamin D3 metabolism.

scholarly journals The neutrophil chemoattractant produced by the rat kidney epithelioid cell line NRK-52E is a protein related to the KC/gro protein

1989 ◽  
Vol 264 (33) ◽  
pp. 19559-19563
Author(s):  
K Watanabe ◽  
K Konishi ◽  
M Fujioka ◽  
S Kinoshita ◽  
H Nakagawa
Keyword(s):  
Cell Line ◽  
Rat Kidney ◽  
Epithelioid Cell

scholarly journals ON THE LOCALIZATION OF SOME PHOSPHATASES IN THREE DIFFERENT SEGMENTS OF THE PROXIMAL TUBULES IN THE RAT KIDNEY

1967 ◽  
Vol 15 (8) ◽  
pp. 456-469 ◽  
Author(s):  
N. O. JACOBSEN ◽  
F. JØRGENSEN ◽  
Å. C. THOMSEN
Keyword(s):  
Adenosine Triphosphate ◽  
Rat Kidney ◽  
Adenosine Monophosphate ◽  
Adenosine Diphosphate ◽  
Proximal Tubules ◽  
Reaction Pattern ◽  
Cytoplasmic Bodies ◽  
Acid And Alkaline Phosphatase ◽  
Convoluted Tubules

The distribution of several phosphatases in three segments of the proximal tubules was studied in frozen sections of glutaraldehyde-fixed rat kidneys. Two segments of the convoluted tubules were identified by in vivo injection of trypan blue. By increasing the concentration of adenosine triphosphate to 3 mM in the Wachstein and Meisel ATPase medium, a clear segmental differentiation in the reaction pattern of the brush border, cytoplasmic bodies and basal infoldings of the proximal tubules was obtained. The specificity of the reaction was investigated by substituting adenosine diphosphate, adenosine monophosphate or β-glycerophosphate for adenosine triphosphate in the incubation medium and by employing cyanide or fluoride as inhibitors. The reaction pattern was also compared with the localization of acid and alkaline phosphatase activities. In addition, the distribution of glucose 6-phosphatase activity was studied which showed differences in the three segments of the proximal tubules.

scholarly journals Erratum: A Novel Model of Surgical Injury in Adult Rat Kidney: A “Pouch Model”

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Natalia O. Litbarg ◽  
Snezana Vujicic ◽  
Suman Setty ◽  
Periannan Sethupathi ◽  
George Dunea ◽  
...  
Keyword(s):  
Rat Kidney ◽  
Adult Rat ◽  
Novel Model ◽  
Surgical Injury

Sepsis induces changes in the expression and distribution of Toll-like receptor 4 in the rat kidney

2006 ◽  
Vol 290 (5) ◽  
pp. F1034-F1043 ◽  
Author(s):  
Tarek M. El-Achkar ◽  
Xiaoping Huang ◽  
Zoya Plotkin ◽  
Ruben M. Sandoval ◽  
Georges J. Rhodes ◽  
...  
Keyword(s):  
Rat Kidney ◽  
Expression Patterns ◽  
Cecal Ligation ◽  
Proximal Tubules ◽  
Microbial Pathogens ◽  
Sprague Dawley ◽  
Renal Vasculature ◽  
Two Photon Microscopy ◽  
Sprague Dawley Rats ◽  
Cellular Signal

Toll-like receptors (TLRs) are now recognized as the major receptors for microbial pathogens on cells of the innate immune system. Recently, TLRs were also identified in many organs including the kidney. However, the cellular distribution and role of these renal TLRs remain largely unknown. In this paper, we investigated the expression of TLR4 in a cecal ligation and puncture (CLP) model of sepsis in Sprague-Dawley rats utilizing fluorescence microscopy. In sham animals, TLR4 was expressed predominantly in Tamm-Horsfall protein (THP)-positive tubules. In CLP animals, TLR4 expression increased markedly in all tubules (proximal and distal), glomeruli, and the renal vasculature. The staining showed a strong apical distribution in all tubules. A moderately less intense cellular signal colocalized partially with the Golgi apparatus. In addition, kidneys from septic rats showed increased expression of CD14 and THP. They each colocalized strongly with TLR4, albeit in different tubular segments. We also imaged the kidneys of live septic animals with two-photon microscopy after fluorescent lipopolysaccharide (LPS) injection. Within 10 min, LPS was seen at the brush border of some proximal tubules. Within 60 min, LPS was fully cytoplasmic in proximal tubules. Conversely, distal tubules showed no LPS uptake. We conclude that TLR4, CD14, and THP have specific renal cellular and tubular expression patterns that are markedly affected by sepsis. Systemic endotoxin can freely access the tubular and cellular sites where these proteins are present. Therefore, locally expressed TLRs and other interacting proteins could potentially modulate the renal response to systemic sepsis.

Primary epithelial cell culture of adult rat kidney, enhancement of cell growth by ammonium acetate

1979 ◽  
Vol 121 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Jules Berman ◽  
Alan Perantoni ◽  
Hester Marie Jackson ◽  
Elizabeth Kingsbury
Keyword(s):  
Cell Culture ◽  
Cell Growth ◽  
Epithelial Cell ◽  
Ammonium Acetate ◽  
Rat Kidney ◽  
Epithelial Cell Culture ◽  
Adult Rat ◽  
Primary Epithelial Cell
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