scholarly journals Cyclin kinase inhibitors are increased during experimental membranous nephropathy: Potential role in limiting glomerular epithelial cell proliferation in vivo

1997 ◽  
Vol 52 (2) ◽  
pp. 404-413 ◽  
Author(s):  
Stuart J. Shankland ◽  
Jürgen Floege ◽  
Susan E. Thomas ◽  
Masaomi Nangaku ◽  
Christian Hugo ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cell ◽  
Membranous Nephropathy ◽  
Potential Role ◽  
Kinase Inhibitors ◽  
Epithelial Cell Proliferation ◽  
Glomerular Epithelial Cell

Proliferation and repair of guinea pig tracheal epithelium after neuropeptide depletion and injury in vivo

1997 ◽  
Vol 273 (6) ◽  
pp. L1235-L1241 ◽  
Author(s):  
John S. Kim ◽  
Valerie S. McKinnis ◽  
Kimberly Adams ◽  
Steven R. White
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cell ◽  
Mucosal Injury ◽  
Nuclear Antigen ◽  
Airway Epithelial ◽  
Epithelial Cell Proliferation ◽  
Injury Site ◽  
And Migration

Neuropeptides stimulate airway epithelial cell proliferation and migration in vitro, but the role of neuropeptides in the repair of the epithelium after injury in vivo is not clear. We studied epithelial proliferation and repair in 83 male Hartley guinea pigs. Animals received capsaicin weekly for 3 wk to deplete airway neuropeptides. One week later, the dorsal aspect of the trachea was injured with a metal stylette. Animals were killed 1 h to 1 wk later, after which epithelial cell proliferation was assessed for the presence of proliferating cell nuclear antigen (PCNA). PCNA labeling was <3% in noninjured animals. PCNA labeling increased substantially in the first 72 h after injury in control animals but was significantly decreased in capsaicin-treated animals within and adjacent to the site of injury. PCNA labeling increased opposite to the injury site in both control and capsaicin animals over the first 72 h. We conclude that neuropeptide depletion significantly attenuates both epithelial cell proliferation and repair in the first 72 h after mechanical injury to the trachea. However, neuropeptide depletion did not slow the ultimate repair of tracheal mucosal injury. Proliferation of epithelial cells in response to injury occurs throughout the airway, even away from the injury site.

The influence of protein fractions from bovine colostrum digested in vivo and in vitro on human intestinal epithelial cell proliferation

2014 ◽  
Vol 81 (1) ◽  
pp. 73-81 ◽  
Author(s):  
Alison J Morgan ◽  
Lisa G Riley ◽  
Paul A Sheehy ◽  
Peter C Wynn
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cell ◽  
Proliferative Activity ◽  
Intestinal Epithelial Cell ◽  
Bovine Colostrum ◽  
Intestinal Epithelial ◽  
Epithelial Cell Proliferation ◽  
Human Intestinal Epithelial Cell

Colostrum consists of a number of biologically active proteins and peptides that influence physiological function and development of a neonate. The present study investigated the biological activity of peptides released from first day bovine colostrum through in vitro and in vivo enzymatic digestion. This was assessed for proliferative activity using a human intestinal epithelial cell line, T84. Digestion of the protein fraction of bovine colostrum in vitro was conducted with the enzymes pepsin, chymosin and trypsin. Pepsin and chymosin digests yielded protein fractions with proliferative activity similar to that observed with undigested colostrum and the positive control foetal calf serum (FCS). In contrast trypsin digestion significantly (P<0·05) decreased colostral proliferative activity when co-cultured with cells when compared with undigested colostrum. The proliferative activity of undigested colostrum protein and abomasal whey protein digesta significantly increased (P<0·05) epithelial cell proliferation in comparison to a synthetic peptide mix. Bovine colostrum protein digested in vivo was collected from different regions of the gastrointestinal tract (GIT) in newborn calves fed either once (n=3 calves) or three times at 12-h intervals (n=3 calves). Digesta collected from the distal duodenum, jejunum and colon of calves fed once, significantly (P<0·05) stimulated cell proliferation in comparison with comparable samples collected from calves fed multiple times. These peptide enriched fractions are likely to yield candidate peptides with potential application for gastrointestinal repair in mammalian species.

scholarly journals Krüppel-like factor 4 is a negative regulator of STAT3-induced glomerular epithelial cell proliferation

JCI Insight ◽  
2018 ◽  
Vol 3 (12) ◽  
Author(s):  
Chelsea C. Estrada ◽  
Praharshasai Paladugu ◽  
Yiqing Guo ◽  
Jesse Pace ◽  
Monica P. Revelo ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cell ◽  
Negative Regulator ◽  
Epithelial Cell Proliferation ◽  
Glomerular Epithelial Cell

scholarly journals Neu Differentiation Factor (NDF), a Dominant Oncogene, Causes Apoptosis In Vitro and In Vivo

1998 ◽  
Vol 188 (8) ◽  
pp. 1535-1539 ◽  
Author(s):  
Stefan Grimm ◽  
Edward J. Weinstein ◽  
Ian M. Krane ◽  
Philip Leder
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cell ◽  
Proliferative Activity ◽  
Epithelial Cell Proliferation ◽  
Potent Inducer ◽  
Proliferative Effect ◽  
Differentiation Factor ◽  
Apoptotic Effect

Neu differentiation factor (NDF, also called neuregulin) is a potent inducer of epithelial cell proliferation and has been shown to induce mammary carcinomas in transgenic mice. Notwithstanding this proliferative effect, we have shown that a novel isoform of NDF can induce apoptosis when overexpressed. Here we report that this property also extends to other NDF isoforms and that the cytoplasmic portion of NDF is largely responsible for the apoptotic effect, whereas the proliferative activity is likely to depend upon the secreted version of NDF. In accordance with these contradictory properties, we find that tumors induced by NDF display extensive apoptosis in vivo. NDF is therefore an oncogene whose deregulation can induce transformation as well as apoptosis.

scholarly journals The cyclin kinase inhibitor p21CIP1/WAF1 limits glomerular epithelial cell proliferation in experimental glomerulonephritis

1999 ◽  
Vol 55 (6) ◽  
pp. 2349-2361 ◽  
Author(s):  
Yoon-Goo Kim ◽  
Charles E. Alpers ◽  
James Brugarolas ◽  
Richard J. Johnson ◽  
William G. Couser ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cell ◽  
Kinase Inhibitor ◽  
Epithelial Cell Proliferation ◽  
Glomerular Epithelial Cell ◽  
Experimental Glomerulonephritis ◽  
Cyclin Kinase Inhibitor
Sign in / Sign up

Export Citation Format

Share Document