scholarly journals Effect of lactate-buffered peritoneal dialysis fluids on human peritoneal mesothelial cell interleukin-6 and prostaglandin synthesis

1995 ◽  
Vol 47 (1) ◽  
pp. 282-293 ◽  
Author(s):  
Janusz Witowski ◽  
Nicholas Topley ◽  
Achim Jörres ◽  
Tomasz Liberek ◽  
Gerald A. Coles ◽  
...  
Keyword(s):  
Peritoneal Dialysis ◽  
Interleukin 6 ◽  
Mesothelial Cell ◽  
Prostaglandin Synthesis ◽  
Peritoneal Mesothelial Cell ◽  
Dialysis Fluids ◽  
Human Peritoneal Mesothelial Cell

scholarly journals Effect of Glucose Degradation Products on Human Peritoneal Mesothelial Cell Function

2000 ◽  
Vol 11 (4) ◽  
pp. 729-739 ◽  
Author(s):  
JANUSZ WITOWSKI ◽  
KATARZYNA KORYBALSKA ◽  
JUSTYNA WISNIEWSKA ◽  
ANDRZEJ BREBOROWICZ ◽  
GERHARD M. GAHL ◽  
...  
Keyword(s):  
Cell Function ◽  
Degradation Products ◽  
Mesothelial Cell ◽  
L929 Cells ◽  
Glucose Degradation Products ◽  
Transformed Cell Lines ◽  
The Impact ◽  
Peritoneal Mesothelial Cell ◽  
Dialysis Fluids ◽  
Human Peritoneal Mesothelial Cell

Abstract. Bioincompatibility of conventional glucose-based peritoneal dialysis fluids (PDF) has been partially attributed to the presence of glucose degradation products (GDP) generated during heat sterilization of PDF. Most previous studies on GDP toxicity were performed on animal and/or transformed cell lines, and the impact of GDP on peritoneal cells remains obscure. The short-term effects of six identified GDP on human peritoneal mesothelial cell (HPMC) functions were examined in comparison to murine L929 fibroblasts. Exposure of HPMC to acetaldehyde, formaldehyde, glyoxal, methylglyoxal, furaldehyde, but not to 5-hydroxymethyl-furfural, resulted in dose-dependent inhibition of cell growth, viability, and interleukin-1 β (IL-1 β)-stimulated IL-6 release; for several GDP, this suppression was significantly greater compared with L929 cells. Although the addition of GDP to culture medium at concentrations found in PDF had no major impact on HPMC function, the exposure of HPMC to filter-sterilized PDF led to a significantly smaller suppression of HPMC proliferation compared to that induced by heat-sterilized PDF. The growth inhibition mediated by filter-sterilized PDF could be increased after the addition of clinically relevant doses of GDP. These effects were equally evident in L929 cells. In conclusion, GDP reveal a significant cytotoxic potential toward HPMC that may be underestimated in test systems using L929 cells. GDP-related toxicity appears to be particularly evident in experimental systems using proliferating cells and the milieu of dialysis fluids. Thus, these observations may bear biologic relevance in vivo where HPMC are repeatedly exposed to GDP-containing PDF for extended periods of time.

scholarly journals Human peritoneal mesothelial cell prostaglandin synthesis: Induction of cyclooxygenase mRNA by peritoneal macrophage-derived cytokines

1994 ◽  
Vol 46 (3) ◽  
pp. 900-909 ◽  
Author(s):  
Nicholas Topley ◽  
Meryl M. Petersen ◽  
Ruth Mackenzie ◽  
Andreas Neubauer ◽  
Eleni Stylianou ◽  
...  
Keyword(s):  
Peritoneal Macrophage ◽  
Mesothelial Cell ◽  
Prostaglandin Synthesis ◽  
Peritoneal Mesothelial Cell ◽  
Human Peritoneal Mesothelial Cell

Mesothelial Toxicity of Peritoneal Dialysis Fluids is Related Primarily to Glucose Degradation Products, Not to Glucose Per Se

2003 ◽  
Vol 23 (4) ◽  
pp. 381-390
Author(s):  
Janusz Witowski ◽  
Thorsten O. Bender ◽  
Justyna Wisniewska–Elnur ◽  
Krzysztof Ksiazek ◽  
Jutta Passlick–Deetjen ◽  
...  
Keyword(s):  
Peritoneal Dialysis ◽  
Degradation Products ◽  
Mesothelial Cell ◽  
Key Factors ◽  
Glucose Degradation Products ◽  
Per Se ◽  
High Concentrations ◽  
And Function ◽  
Peritoneal Mesothelial Cell ◽  
Dialysis Fluids

♦ Objectives High concentrations of glucose and/or formation of glucose degradation products (GDPs) during heat sterilization of peritoneal dialysis fluids (PDFs) are believed to be key factors in the limited biocompatibility of PDFs. We have previously shown that several identified GDPs can specifically impair human peritoneal mesothelial cell (HPMC) function. In the present study we aimed at differentiating the respective roles of glucose and GDPs in the toxicity of PDF to mesothelial cells. ♦ Methods HPMCs were acutely pre-exposed to or incubated chronically in the presence of pH-neutral PDF sterilized by either heat (H-PDF) or filtration (F-PDF). In addition, HPMCs were treated with commercially available H-PDF manufactured either conventionally, that is, in single-chamber containers, or using novel dual-chamber bags that help to substantially decrease GDP formation. Functional assessment of HPMCs included viability, release of interleukin (IL)-6, and proliferation. ♦ Results Viability and release of IL-6 from HPMCs pretreated with H-PDF (pH 7.3) for 1 to 4 hours were significantly reduced compared to cells exposed to corresponding F-PDF. Incubation in medium mixed (1:1) with H-PDF considerably impaired growth of HPMCs, and over a period of 10 days gradually decreased both the viability of HPMCs and their ability to generate IL-6. These effects were either absent from or significantly less in HPMCs exposed to F-PDF. Similar differences were observed when commercial GDP-containing H-PDFs were compared with newly designed H-PDFs free of GDPs. ♦ Conclusions Impaired viability and function of HPMCs exposed to glucose-containing pH-neutral PDF is related predominantly to the presence of GDP and, to a significantly lesser extent, to the presence of glucose per se. Prevention of GDP formation during auto-claving markedly improves the biocompatibility of H-PDF with HPMCs.

scholarly journals Pentoxifylline inhibits human peritoneal mesothelial cell growth and collagen synthesis: Effects on TGF-β

2000 ◽  
Vol 57 (6) ◽  
pp. 2626-2633 ◽  
Author(s):  
Cheng-Chung Fang ◽  
Chung-Jen Yen ◽  
Yung-Ming Chen ◽  
Ren-Shi Shyu ◽  
Tun-Jun Tsai ◽  
...  
Keyword(s):  
Cell Growth ◽  
Collagen Synthesis ◽  
Mesothelial Cell ◽  
Peritoneal Mesothelial Cell ◽  
Human Peritoneal Mesothelial Cell

Feasibility of Mesothelial Transplantation during Experimental Peritoneal Dialysis and Peritonitis

2007 ◽  
Vol 30 (6) ◽  
pp. 513-519 ◽  
Author(s):  
L.H.P. Hekking ◽  
J. Van Den Born
Keyword(s):  
Peritoneal Dialysis ◽  
Cell Transplantation ◽  
Cell Layer ◽  
Adhesion Formation ◽  
Mesothelial Cell ◽  
Continuous Exposure ◽  
Peritoneal Membrane ◽  
Trouble Shooting ◽  
Transplantation Experiments ◽  
Dialysis Fluids

The mesothelial cell layer lining the peritoneum orchestrates peritoneal homeostasis. Continuous exposure to peritoneal dialysis fluids and episodes of peritonitis may damage the monolayer irreversibly, eventually leading to adhesion formation and fibrosis/sclerosis of the peritoneum. Autologous mesothelial cell transplantation is thought to be one of the options to reduce dysfunction of the peritoneal membrane. In this article we will review the mesothelial cell transplantation experiments performed in the field of peritoneal dialysis and peritonitis. In addition we will focus on the trouble shooting using cultured autologous mesothelial cells for transplantation.

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