scholarly journals Alpha-adrenoceptors and angiotensinogen gene expression in opossum kidney cells

1995 ◽  
Vol 48 (1) ◽  
pp. 139-145 ◽  
Author(s):  
Tian-Tian Wang ◽  
Silvana Lachance ◽  
Aline Delalandre ◽  
Serge Carrière ◽  
John S.D. Chan
Keyword(s):  
Gene Expression ◽  
Kidney Cells ◽  
Opossum Kidney ◽  
Alpha Adrenoceptors ◽  
Angiotensinogen Gene ◽  
Opossum Kidney Cells

scholarly journals β-Adrenoceptors and dexamethasone synergistically stimulate the expression of the angiotensinogen gene in opossum kidney cells

1996 ◽  
Vol 50 (1) ◽  
pp. 94-101 ◽  
Author(s):  
Ming Ming ◽  
Wynnie Chan ◽  
Tian T. Wang ◽  
Kenneth D. Roberts ◽  
Michel Bouvier ◽  
...  
Keyword(s):  
Kidney Cells ◽  
Opossum Kidney ◽  
Angiotensinogen Gene ◽  
Opossum Kidney Cells

scholarly journals Isoproterenol and 8-bromo-cyclic adenosine monophosphate stimulate the expression of the angiotensinogen gene in opossum kidney cells

1994 ◽  
Vol 46 (3) ◽  
pp. 703-710 ◽  
Author(s):  
Tian-Tian Wang ◽  
Ming Chen ◽  
Silvana Lachance ◽  
Aline Delalandre ◽  
Serge Carrière ◽  
...  
Keyword(s):  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Kidney Cells ◽  
Opossum Kidney ◽  
Angiotensinogen Gene ◽  
Opossum Kidney Cells

scholarly journals Effect of glucose on the expression of the angiotensinogen gene in opossum kidney cells

1998 ◽  
Vol 53 (2) ◽  
pp. 312-319 ◽  
Author(s):  
Tian-Tian Wang ◽  
Xiao-Hua Wu ◽  
Shao-Ling Zhang ◽  
John S.D. Chan
Keyword(s):  
Kidney Cells ◽  
Opossum Kidney ◽  
Angiotensinogen Gene ◽  
Opossum Kidney Cells ◽  
Effect Of Glucose

Expression of the angiotensinogen gene is synergistically stimulated by 8-BrcAMP and Dex in opossum kidney cells

1995 ◽  
Vol 268 (1) ◽  
pp. R105-R111 ◽  
Author(s):  
M. Ming ◽  
T. T. Wang ◽  
S. Lachance ◽  
A. Delalandre ◽  
S. Carriere ◽  
...  
Keyword(s):  
Fusion Gene ◽  
Dependent Manner ◽  
Fusion Genes ◽  
Opossum Kidney ◽  
Ok Cells ◽  
Angiotensinogen Gene ◽  
Opossum Kidney Cells ◽  
Dependent Protein ◽  
Flanking Region ◽  
Responsive Element

We transiently transfected fusion genes with the 5'-flanking region of the angiotensinogen gene linked to a bacterial chloramphenicol acetyltransferase (CAT) coding sequence as a reporter into opossum kidney (OK) cells. The addition of 8-bromoadenosine 3',5'-cyclic monophosphate (8-BrcAMP) (10(-3)-10(-7) M) or forskolin (10(-9)-10(-5) M) stimulated the expression of the plasmid pOCAT [angiotensinogen nucleotide (N) -1498/+18] fusion gene in OK cells in a dose-dependent manner. The addition of dexamethasone (Dex) (10(-6) M) further enhanced the stimulatory effect of 8-BrcAMP or forskolin, whereas the addition of (R)-p-adenosine 3',5'-cyclic monophosphorothioate [(Rp)-cAMP[S], an inhibitor of cAMP-dependent protein kinase A, I and II] blocked the stimulatory effect of 8-BrcAMP. Furthermore, the addition of 8-BrcAMP (10(-3) M) or Dex (10(-6) M) or a combination of both stimulated the expression of pOCAT (angiotensinogen N -1138/+18), pOCAT (angiotensinogen N -960/+18), pOCAT (angiotensinogen N -814/+18), and pOCAT (angiotensinogen N -688/+18), but had no effect on the expression of pOCAT (angiotensinogen N -280/+18), pOCAT (angiotensinogen N -198/+18), pOCAT (angiotensinogen N -110/+18), pOCAT (angiotensinogen N -53/+18), and pOCAT (angiotensinogen N -35/+18). To further localize the putative cAMP-responsive element (CRE) in the angiotensinogen gene, we constructed fusion genes by inserting the DNA fragments angiotensinogen N -814 to N -689, angiotensinogen N -814 to N -761, and angiotensinogen N -760 to N -689 of the 5'-flanking region of the angiotensinogen gene upstream of the thymidine kinase (TK) promoter fused to a CAT gene and introduced them into OK cells.(ABSTRACT TRUNCATED AT 250 WORDS)

Evidence for interference of vitamin D with PTH/PTHrP receptor expression in opossum kidney cells

1998 ◽  
Vol 436 (2) ◽  
pp. 289-294 ◽  
Author(s):  
H. Wald ◽  
Michal Dranitzki-Elhalel ◽  
R. Backenroth ◽  
Mordecai M. Popovtzer
Keyword(s):  
Vitamin D ◽  
Receptor Expression ◽  
Kidney Cells ◽  
Opossum Kidney ◽  
Opossum Kidney Cells ◽  
Pthrp Receptor
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