scholarly journals Eicosanoid production by isolated glomeruli of rats with unilateral ureteral obstruction

1990 ◽  
Vol 37 (6) ◽  
pp. 1528-1535 ◽  
Author(s):  
Hiroyuki Yanagisawa ◽  
Jeremiah Morrissey ◽  
Aubrey R. Morrison ◽  
Saulo Klahr
Keyword(s):  
Ureteral Obstruction ◽  
Unilateral Ureteral Obstruction ◽  
Isolated Glomeruli ◽  
Eicosanoid Production

Mechanism of enhanced eicosanoid production by isolated glomeruli from rats with bilateral ureteral obstruction

1991 ◽  
Vol 261 (2) ◽  
pp. F248-F255 ◽  
Author(s):  
H. Yanagisawa ◽  
J. Morrissey ◽  
S. Klahr
Keyword(s):  
Ureteral Obstruction ◽  
Ace Inhibitor ◽  
Converting Enzyme ◽  
Isolated Glomeruli ◽  
Eicosanoid Production ◽  
Thromboxane Synthase Inhibitor ◽  
Pg E2 ◽  
Synthase Inhibitor

Isolated glomeruli from rats with bilateral ureteral obstruction (BUO) of 24-h duration produced significantly greater amounts of prostaglandin (PG) E2 and 6-keto-PGF1 alpha in vitro than glomeruli from sham-operated control (SOC) rats. This increase was abolished by the angiotensin-converting enzyme (ACE) inhibitor, enalaprilat, given in vivo. To elucidate the mechanisms responsible for enhanced eicosanoid production by glomeruli from rats with BUO, we measured the activities of phospholipase (PL) A2 and C and cyclooxygenase in glomeruli isolated from SOC and BUO rats. L-alpha-Phosphatidylcholine (PC)-specific and L-alpha-phosphatidylethanolamine (PE)-specific PLA2 activities were significantly greater in glomerular membranes from rats with BUO than from SOC rats. Likewise, both the activity and amount of cyclooxygenase were significantly greater in glomerular membranes of rats with BUO. Cyclooxygenase and the PE-specific PLA2 in glomerular membranes of rats with BUO remained at the levels seen in SOC rats when animals were treated in vivo before BUO with the ACE inhibitor, enalaprilat, and the thromboxane synthase inhibitor, OKY-046. Thus inhibition of vasoconstrictor formation leads to subsequent inhibition of vasodilator formation. In contrast to PE-specific PLA2, PC-specific PLA2 activities were further increased in glomerular membranes from both SOC and BUO rats pretreated with the two drugs.s The activity of phosphatidylinositol 4,5-bisphosphate-specific phospholipase C (PIP2 PLC) was significantly decreased in glomeruli from rats with BUO compared with SOC rats. We conclude that the increased synthesis of vasodilatory eicosanoids by glomeruli from rats with BUO may be mediated by enhanced activities of PE-specific PLA2 and cyclooxygenase, which are apparently stimulated by the vasoconstrictors angiotensin and thromboxane.

Role of ANG II in eicosanoid production by isolated glomeruli from rats with bilateral ureteral obstruction

1990 ◽  
Vol 258 (1) ◽  
pp. F85-F93 ◽  
Author(s):  
H. Yanagisawa ◽  
J. Morrissey ◽  
A. R. Morrison ◽  
M. L. Purkerson ◽  
S. Klahr
Keyword(s):  
Ureteral Obstruction ◽  
Ang Ii ◽  
Isolated Glomeruli ◽  
Angiotensin I Converting Enzyme ◽  
Eicosanoid Production ◽  
Prostanoid Production ◽  
Pg E2

The production of prostaglandin (PG) E2, 6-keto-PGF1 alpha, and thromboxane B2 (TxB2) under basal conditions and after exposure to angiotensin II (ANG II) or arginine vasopressin (AVP) was examined in vitro in isolated glomeruli. The glomeruli were obtained from control rats and rats with bilateral ureteral obstruction (BUO) of 24-h duration that were pretreated or not with an inhibitor of the angiotensin I converting enzyme (ACE). Basal prostanoid production was greater in isolated glomeruli from BUO rats than in controls. Administration of an ACE inhibitor, enalaprilat, given in vivo returned basal prostanoid production by isolated glomeruli of BUO rats to levels seen in glomeruli of control rats. The prostanoid production in response to addition of ANG II or AVP in vitro was blunted in glomeruli from BUO rats, but the response was restored to “normal” after blockade of ANG II synthesis in vivo in BUO rats. Blockade of ANG II synthesis in vivo did not affect prostanoid synthesis by isolated glomeruli of control rats. The prostanoid generation in response to addition of both ANG II and arachidonic acid in vitro compared with ANG II addition alone was not significantly stimulated in glomeruli from BUO rats. In contrast, it was significantly stimulated in glomeruli of control rats. The results indicate that endogenous ANG II has an important role in the increased synthesis of prostanoids found in isolated glomeruli of rats with BUO and that the in vitro prostanoid production in response to ANG II and AVP can be restored to normal when the synthesis of ANG II is inhibited in vivo.(ABSTRACT TRUNCATED AT 250 WORDS)

Evaluation of preoperative ultrasonographic parameters to predict renal recovery in long-term survivors after treatment of feline ureteral obstructions: 2012–2019

2021 ◽  
pp. 1098612X2110236
Author(s):  
Elisa P McEntee ◽  
Allyson C Berent ◽  
Chick Weisse ◽  
Alexandre Le Roux ◽  
Kenneth Lamb
Keyword(s):  
Serum Creatinine ◽  
Ultrasound Imaging ◽  
Ureteral Obstruction ◽  
Unilateral Ureteral Obstruction ◽  
Biochemical Data ◽  
Preoperative Imaging ◽  
Long Term Outcome ◽  
Preoperative Ultrasound ◽  
Imaging Characteristics

Objectives The aim of this study was to determine whether preoperative ultrasound imaging characteristic(s) in cats suffering from unilateral benign ureteral obstructions are predictive of outcome after successful renal decompression with a subcutaneous ureteral bypass (SUB) device. Methods This was a retrospective study of 37 cats with unilateral, benign ureteral obstruction. Preoperative imaging characteristics (including renal pelvis diameter, parenchymal thickness [transverse plane], renal length and pelvic size:overall renal size) and biochemical data were evaluated for all cats diagnosed with a unilateral ureteral obstruction treated with a SUB device. Any patient with bilateral obstructions or documented bacteriuria/infection in the data collection period was excluded. All patients were followed between 3 and 6 months postoperatively to obtain postoperative biochemical data. Long-term outcome was defined as serum creatinine concentration at 3–6 months postoperatively. Results No preoperative imaging characteristics or biochemical findings were found to be significantly associated with long-term serum creatinine concentrations. The length of the kidney was found to be associated with change in blood urea nitrogen and creatinine with decompression but not with long-term renal values. Conclusions and relevance In this study, long-term renal function based on preoperative ultrasound imaging findings could not be predicted in cats with unilateral ureteral obstruction, regardless of the severity of the biochemical parameters, renal pelvic dilation (large or small pelvis), kidney size or thickness of renal parenchyma assessed.

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