scholarly journals Application of cold flush preservation to in vitro microperfusion studies of kidney tubules

1985 ◽  
Vol 28 (6) ◽  
pp. 982-984 ◽  
Author(s):  
Susan C. Pirie ◽  
David J. Potts
Keyword(s):  
Kidney Tubules ◽  
In Vitro Microperfusion

Chloride flux in rabbit kidney tubules in vitro

1967 ◽  
Vol 213 (5) ◽  
pp. 1249-1253 ◽  
Author(s):  
M Abramow ◽  
MB Burg ◽  
J Orloff
Keyword(s):  
Rabbit Kidney ◽  
Kidney Tubules ◽  
Chloride Flux

scholarly journals Bioengineered Cystinotic Kidney Tubules Recapitulate a Nephropathic Phenotype

Cells ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 177
Author(s):  
Elena Sendino Garví ◽  
Rosalinde Masereeuw ◽  
Manoe J. Janssen
Keyword(s):  
Cell Culture ◽  
Proximal Tubule ◽  
Vesicle Trafficking ◽  
Severe Disease ◽  
In Vitro Models ◽  
Nephropathic Cystinosis ◽  
Kidney Tubules ◽  
Renal Fanconi Syndrome ◽  
Dimensional Cell

Nephropathic cystinosis is a rare and severe disease caused by disruptions in the CTNS gene. Cystinosis is characterized by lysosomal cystine accumulation, vesicle trafficking impairment, oxidative stress, and apoptosis. Additionally, cystinotic patients exhibit weakening and leakage of the proximal tubular segment of the nephrons, leading to renal Fanconi syndrome and kidney failure early in life. Current in vitro cystinotic models cannot recapitulate all clinical features of the disease which limits their translational value. Therefore, the development of novel, complex in vitro models that better mimic the disease and exhibit characteristics not compatible with 2-dimensional cell culture is of crucial importance for novel therapies development. In this study, we developed a 3-dimensional bioengineered model of nephropathic cystinosis by culturing conditionally immortalized proximal tubule epithelial cells (ciPTECs) on hollow fiber membranes (HFM). Cystinotic kidney tubules showed lysosomal cystine accumulation, increased autophagy and vesicle trafficking deterioration, the impairment of several metabolic pathways, and the disruption of the epithelial monolayer tightness as compared to control kidney tubules. In particular, the loss of monolayer organization and leakage could be mimicked with the use of the cystinotic kidney tubules, which has not been possible before, using the standard 2-dimensional cell culture. Overall, bioengineered cystinotic kidney tubules recapitulate better the nephropathic phenotype at a molecular, structural, and functional proximal tubule level compared to 2-dimensional cell cultures.

Separation of avian kidney tubules and glomeruli for in vitro culture

1982 ◽  
Vol 7 (3) ◽  
pp. 97-101
Author(s):  
Charles F. Reich ◽  
Robert A. Bonar
Keyword(s):  
In Vitro Culture ◽  
Kidney Tubules ◽  
Avian Kidney

Glucocorticoid stimulation of Na-K-ATPase in superfused distal segments of kidney tubules in vitro

1982 ◽  
Vol 243 (5) ◽  
pp. F463-F470 ◽  
Author(s):  
B. M. Rayson ◽  
I. S. Edelman
Keyword(s):  
Atpase Activity ◽  
Time Course ◽  
Rat Kidney ◽  
Maximal Effect ◽  
Kidney Tubules ◽  
Response Data ◽  
The One ◽  
Induced Changes

The ability of glucocorticoids to regulate Na-K-ATPase activity directly was assessed in separated rat kidney tubules derived from the distal nephron. These tubules were superfused under sterile conditions and maintained in a viable condition for at least 24 h in a newly devised apparatus. Viability was assessed by measuring O2 consumption, protein/DNA ratios, and Na-K-ATPase and Mg-ATPase activities. At a concentration of 10(-8) M, dexamethasone elicited a 27% increase in tubular Na-K-ATPase activity in 6 h and a 32% increase in 24 h. In a separate series, assayed at 24 h, the maximal effect was obtained at a dexamethasone concentration of less than 10(-8) M, and by inspection half-maximal stimulation was obtained at approximately 10(-9) M. At a concentration of 10(-7) M, 17 beta-estradiol, testosterone, progesterone, and deoxycorticosterone acetate had no significant effect on tubular Na-K-ATPase activity. These results as well as the time-course and dose-response data imply that the response is mediated by the glucocorticoid receptor pathway. Since the magnitude of response in vitro was similar to the one obtained after injection of dexamethasone in vivo, much if not all of the action appears to be direct and independent of glucocorticoid-induced changes in the filtered Na+ load.

Prenatal glucocorticoids stimulate neonatal juxtamedullary proximal convoluted tubule acidification

1991 ◽  
Vol 261 (5) ◽  
pp. F746-F752 ◽  
Author(s):  
M. Baum ◽  
R. Quigley
Keyword(s):  
Glucose Transport ◽  
Intracellular Ph ◽  
Proximal Convoluted Tubule ◽  
Proton Flux ◽  
Control Group ◽  
Urine Ph ◽  
Dexamethasone Group ◽  
Volume Absorption ◽  
In Vitro Microperfusion

The rate of neonatal proximal convoluted tubule (PCT) HCO3 absorption is lower than that of adult animals. The present in vitro microperfusion study examined whether prenatal dexamethasone (60 micrograms/kg daily to the doe for 3 days before delivery) would accelerate the maturation of neonatal juxtamedullary PCT acidification. Control neonates studied within 48 h of birth had a urine pH of 7.06 +/- 0.15 and a urine HCO3 concentration of 34.3 +/- 7.0 meq/l. Animals receiving dexamethasone had a urine pH of 6.47 +/- 0.11 and a urine HCO3 concentration of 10.1 +/- 4.0 meq/l, both of which were significantly lower than control (P less than 0.01). In juxtamedullary PCTs perfused in vitro, volume absorption was 0.27 +/- 0.03 nl.mm-1.min-1 in controls and 0.39 +/- 0.02 nl.mm-1.min-1 in dexamethasone-treated animals (P less than 0.05). HCO3 absorption was stimulated in the dexamethasone group (52.6 +/- 4.6 vs. 34.1 +/- 6.3 pmol.mm-1.min-1, P less than 0.05); however, glucose transport was not significantly affected (24.8 +/- 1.3 in dexamethasone vs. 21.5 +/- 3.5 pmol.mm-1.min-1 in controls). Intracellular pH was measured using 2',7'-bis(carboxyethyl)-5(6)-carboxyflourescin to examine whether prenatal dexamethasone stimulated the apical Na(+)-H+ antiporter and the basolateral Na(HCO3)3 symporter. Apical Na(+)-H+ antiporter proton flux was 108.5 +/- 14.2 pmol.mm-1.min-1 in the control group and 250.7 +/- 31.3 pmol.mm-1.min-1 in the dexamethasone group (P less than 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Generation of easily accessible human kidney tubules on two-dimensional surfaces in vitro

2010 ◽  
Vol 15 (6) ◽  
pp. 1287-1298 ◽  
Author(s):  
Huishi Zhang ◽  
Samantha Fong-Ting Lau ◽  
Ber Fong Heng ◽  
Pei Yun Teo ◽  
P. K. D. Thilini Alahakoon ◽  
...  
Keyword(s):  
Human Kidney ◽  
Two Dimensional ◽  
Kidney Tubules

Effect of vanadate on the renal accumulation of p-aminohippurate in the rabbit kidney tubules in vitro

1981 ◽  
Vol 30 (15) ◽  
pp. 2141-2146 ◽  
Author(s):  
M.Iqbal Sheikh ◽  
Jan Maxild ◽  
Jesper V. Møller
Keyword(s):  
Rabbit Kidney ◽  
Kidney Tubules

Excretion of phenol red by the Necturus kidney

1979 ◽  
Vol 236 (5) ◽  
pp. F442-F447 ◽  
Author(s):  
G. A. Tanner ◽  
P. K. Carmines ◽  
W. B. Kinter
Keyword(s):  
Ringer Solution ◽  
Phenol Red ◽  
Kidney Tubules ◽  
Clearance Ratio ◽  
Frog Kidney ◽  
Kidney Slices ◽  
Necturus Maculosus ◽  
Thin Slices ◽  
Secretory System

Phenol red (phenolsulfonphthalein, PSP) is thought to be secreted by proximal kidney tubules in all vertebrates. The present study examined PSP transport by the kidney of the salamander, Necturus maculosus. In Necturus kidneys perfused with oxygenated Ringer solution, the PSP/creatinine clearance ratio was unity. Perfusion with 1 mM octanoate converted net p-aminohippurate (PAH) reabsorption to net secretion, but had no effect on PSP. In seven urethan-anesthetized Necturi, the PSP/inulin clearance ratio averaged 0.85 +/- 0.21 (SD), not significantly different from unity. Thin slices from Necturus kidneys incubated in vitro for 2 h failed to accumulate PSP; slice-to-medium (S/M) concentration ratios averaged 0.8 +/- 0.2 (n = 6). With frog kidney slices, (S/M)PSP was 9.6 +/- 1.4 (n = 6). Necturus kidney slices accumulated PAH ((S/M)PAH = 4.1 +/- 0.7) (n = 6), but uptake was not inhibited by 1 mM PSP. We conclude that Necturus kidney tubules transport PAH, but do not transport PSP. These results are consistent with the hypothesis that the organic acid secretory system in most animals involves several carriers.

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